TY  - JOUR
TI  - Antibiotic Resistance of Gram-negative Heterotrophic Bacteria on the Marine Sediments of the Black Sea-Comparison of CLSI and EUCAST on the Environmental Isolates
AB  - Marine sediments are the important reservoirs of antibiotics and resistant genes. Antibiotic resistance becomes a global concern today and bacteria from marine sediments show resistance to multiple microbial agents. This study investigates antibiotic resistance of Gram-negative heterotrophic bacteria from the marine sediment of the Black Sea. The samples were gathered from various sampling locations on the marine environments of the Black Sea between May 2019 and February 2020. The VITEK 2 system was utilized to detect the MIC values against different antibiotics automatically. The MIC values were ranged from 0.12 µg/ml to 80 µg/ml. The highest resistance ratios among all antibiotics were detected as 90% in CLSI and 81% in EUCAST against cefazolin. In general, the resistance ratios were determined as 13.4% in CLSI and 26.1% in EUCAST. All isolates were detected as susceptible to meropenem, cefepime, ceftazidime, colistin, ertapenem, and piperacillin/tazobactam. MAR indexes were ranged between 0 and 0.45. MAR index were detected above or equal the value of 0.2 as 27% in CLSI and 81% in EUCAST among all isolates. This study shows that EUCAST may be better than CLSI in terms of showing the antibiotic exposure among natural isolates and the MAR indexes may be a useful tool for defining the environmental status. The results of this study indicate that antibiotic resistance may be a serious concern in sediments of the Black Sea.
AU  - kalkan, samet
DO  - 10.35229/jaes.1039437
PY  - 2022
JO  - JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES
VL  - 7
IS  - 2
SN  - 2548-0006
SP  - 163
EP  - 170
DB  - TRDizin
UR  - http://search/yayin/detay/533146
ER  -