Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray
Yıl: 2022 Cilt: 23 Sayı: 2 Sayfa Aralığı: 214 - 219 Metin Dili: İngilizce DOI: 10.4274/meandros.galenos.2021.87004 İndeks Tarihi: 18-07-2022
Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray
Öz: Objective: To investigate the regenerative effects of adipose-derived mesenchymal
stem cells (ADMSCs) in immortalized human keratinocyte cells (HaCaT) exposed to
ultraviolet B (UVB) rays.
Materials and Methods: Epidermal cells were interacted with UVB rays. Then,
treatment was applied to ADMSC mediums prepared in different concentrations.
Cells that did not receive UVB light were included in the study as a control.
Results: With the application of human adipose tissue mesenchymal stem cells
to HaCaT cells with different concentrations of media prepared, cell viability
increased in both cells that received UVB radiation and those that did not. It has
been observed that the said increases are more pronounced, especially in cells that
have received UVB rays, reaching the maximum value at 100%, which is the highest
stem cell medium concentration. ADMSC medium also had an increasing effect
on the migration of UVB (+) cells. In UVB (+) cells, decreased levels of myeloid
cell leukemia 1 protein (Mcl-1) and phosphorylated extracellular signal-regulated
kinases (p-ERK 1/2) and dose-proportional increases were observed after treatment
with stem cell medium.
Conclusion: It has been determined that some factors in the ADMSC medium,
especially in keratinocytes that have received UVB rays, positively support
proliferation and show reparative and rejuvenating functions.
Anahtar Kelime: UVB Işınına Maruz Kalan Epidermal Hücrelerde İnsan Adipoz Doku Mezenkimal Kök Hücrelerinin Onarıcı ve Canlandırıcı Etkilerinin Araştırılması
Öz: Amaç: Bu çalışmanın amacı ultraviyole B (UVB) ışınlarının etkisi altında bırakılan
immortalize insan keratinosit hücrelerinde (HaCaT), insan adipoz doku mezenkimal
kök hücrelerinin (ADMSC) yenileyici etkilerinin araştırılmasıdır.
Gereç ve Yöntemler: Epidermal hücreler, UVB ışınları ile etkileştirildi. Daha sonra
farklı derişimlerde hazırlanmış olan ADMSC medyumları ile tedavi uygulandı. UVB
ışınını almamış olan hücreler, kontrol amacıyla çalışmada yer aldı.
Bulgular: ADMSC farklı konsantrasyonlarda hazırlanmış olan ortam medyumlarının
HaCaT hücrelerine uygulanmasıyla birlikte hem UVB ışını alan, hem de UVB ışını
almamış olan hücrelerde hücre canlılığının arttığı saptanmıştır. Söz konusu artışların
özellikle UVB ışını almış olan hücrelerde daha belirgin düzeyde olduğu, en yüksek
kök hücre ortam medyumu konsantrasyonu olan %100’lükte maksimum değere ulaştığı görülmüştür. ADMSC medyumu, UVB (+) hücrelerin migrasyonunda da artırıcı yönde etki yapmıştır. UVB (+) hücrelerde,
myeloid hücre lösemi 1 proteni (Mcl-1) ile fosforillenmiş ekstraselüler sinyalle düzenlenen kinazların (p-ERK 1/2) azalan düzeylerinde,
kök hücre ortam medyumu ile muameleden sonra doz ile doğru orantılı artışlar görülmüştür.
Sonuç: Özellikle UVB ışını almış olan keratinositlerde, ADMSC ortam medyumunda bulunan etmenlerin proliferasyonu olumlu yönde
desteklediği, onarıcı ve canlandırıcı fonksiyonlar gösterdiği saptanmıştır
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA | ARSLAN Y, YENİSEY Ç (2022). Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. , 214 - 219. 10.4274/meandros.galenos.2021.87004 |
Chicago | ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. (2022): 214 - 219. 10.4274/meandros.galenos.2021.87004 |
MLA | ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. , 2022, ss.214 - 219. 10.4274/meandros.galenos.2021.87004 |
AMA | ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. . 2022; 214 - 219. 10.4274/meandros.galenos.2021.87004 |
Vancouver | ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. . 2022; 214 - 219. 10.4274/meandros.galenos.2021.87004 |
IEEE | ARSLAN Y,YENİSEY Ç "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray." , ss.214 - 219, 2022. 10.4274/meandros.galenos.2021.87004 |
ISNAD | ARSLAN, Yasemin Tin - YENİSEY, Çiğdem. "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray". (2022), 214-219. https://doi.org/10.4274/meandros.galenos.2021.87004 |
APA | ARSLAN Y, YENİSEY Ç (2022). Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal, 23(2), 214 - 219. 10.4274/meandros.galenos.2021.87004 |
Chicago | ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal 23, no.2 (2022): 214 - 219. 10.4274/meandros.galenos.2021.87004 |
MLA | ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal, vol.23, no.2, 2022, ss.214 - 219. 10.4274/meandros.galenos.2021.87004 |
AMA | ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal. 2022; 23(2): 214 - 219. 10.4274/meandros.galenos.2021.87004 |
Vancouver | ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal. 2022; 23(2): 214 - 219. 10.4274/meandros.galenos.2021.87004 |
IEEE | ARSLAN Y,YENİSEY Ç "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray." Meandros Medical And Dental Journal, 23, ss.214 - 219, 2022. 10.4274/meandros.galenos.2021.87004 |
ISNAD | ARSLAN, Yasemin Tin - YENİSEY, Çiğdem. "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray". Meandros Medical And Dental Journal 23/2 (2022), 214-219. https://doi.org/10.4274/meandros.galenos.2021.87004 |