Yıl: 2022 Cilt: 23 Sayı: 2 Sayfa Aralığı: 214 - 219 Metin Dili: İngilizce DOI: 10.4274/meandros.galenos.2021.87004 İndeks Tarihi: 18-07-2022

Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray

Öz:
Objective: To investigate the regenerative effects of adipose-derived mesenchymal stem cells (ADMSCs) in immortalized human keratinocyte cells (HaCaT) exposed to ultraviolet B (UVB) rays. Materials and Methods: Epidermal cells were interacted with UVB rays. Then, treatment was applied to ADMSC mediums prepared in different concentrations. Cells that did not receive UVB light were included in the study as a control. Results: With the application of human adipose tissue mesenchymal stem cells to HaCaT cells with different concentrations of media prepared, cell viability increased in both cells that received UVB radiation and those that did not. It has been observed that the said increases are more pronounced, especially in cells that have received UVB rays, reaching the maximum value at 100%, which is the highest stem cell medium concentration. ADMSC medium also had an increasing effect on the migration of UVB (+) cells. In UVB (+) cells, decreased levels of myeloid cell leukemia 1 protein (Mcl-1) and phosphorylated extracellular signal-regulated kinases (p-ERK 1/2) and dose-proportional increases were observed after treatment with stem cell medium. Conclusion: It has been determined that some factors in the ADMSC medium, especially in keratinocytes that have received UVB rays, positively support proliferation and show reparative and rejuvenating functions.
Anahtar Kelime:

UVB Işınına Maruz Kalan Epidermal Hücrelerde İnsan Adipoz Doku Mezenkimal Kök Hücrelerinin Onarıcı ve Canlandırıcı Etkilerinin Araştırılması

Öz:
Amaç: Bu çalışmanın amacı ultraviyole B (UVB) ışınlarının etkisi altında bırakılan immortalize insan keratinosit hücrelerinde (HaCaT), insan adipoz doku mezenkimal kök hücrelerinin (ADMSC) yenileyici etkilerinin araştırılmasıdır. Gereç ve Yöntemler: Epidermal hücreler, UVB ışınları ile etkileştirildi. Daha sonra farklı derişimlerde hazırlanmış olan ADMSC medyumları ile tedavi uygulandı. UVB ışınını almamış olan hücreler, kontrol amacıyla çalışmada yer aldı. Bulgular: ADMSC farklı konsantrasyonlarda hazırlanmış olan ortam medyumlarının HaCaT hücrelerine uygulanmasıyla birlikte hem UVB ışını alan, hem de UVB ışını almamış olan hücrelerde hücre canlılığının arttığı saptanmıştır. Söz konusu artışların özellikle UVB ışını almış olan hücrelerde daha belirgin düzeyde olduğu, en yüksek kök hücre ortam medyumu konsantrasyonu olan %100’lükte maksimum değere ulaştığı görülmüştür. ADMSC medyumu, UVB (+) hücrelerin migrasyonunda da artırıcı yönde etki yapmıştır. UVB (+) hücrelerde, myeloid hücre lösemi 1 proteni (Mcl-1) ile fosforillenmiş ekstraselüler sinyalle düzenlenen kinazların (p-ERK 1/2) azalan düzeylerinde, kök hücre ortam medyumu ile muameleden sonra doz ile doğru orantılı artışlar görülmüştür. Sonuç: Özellikle UVB ışını almış olan keratinositlerde, ADMSC ortam medyumunda bulunan etmenlerin proliferasyonu olumlu yönde desteklediği, onarıcı ve canlandırıcı fonksiyonlar gösterdiği saptanmıştır
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ARSLAN Y, YENİSEY Ç (2022). Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. , 214 - 219. 10.4274/meandros.galenos.2021.87004
Chicago ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. (2022): 214 - 219. 10.4274/meandros.galenos.2021.87004
MLA ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. , 2022, ss.214 - 219. 10.4274/meandros.galenos.2021.87004
AMA ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. . 2022; 214 - 219. 10.4274/meandros.galenos.2021.87004
Vancouver ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. . 2022; 214 - 219. 10.4274/meandros.galenos.2021.87004
IEEE ARSLAN Y,YENİSEY Ç "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray." , ss.214 - 219, 2022. 10.4274/meandros.galenos.2021.87004
ISNAD ARSLAN, Yasemin Tin - YENİSEY, Çiğdem. "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray". (2022), 214-219. https://doi.org/10.4274/meandros.galenos.2021.87004
APA ARSLAN Y, YENİSEY Ç (2022). Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal, 23(2), 214 - 219. 10.4274/meandros.galenos.2021.87004
Chicago ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal 23, no.2 (2022): 214 - 219. 10.4274/meandros.galenos.2021.87004
MLA ARSLAN Yasemin Tin,YENİSEY Çiğdem Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal, vol.23, no.2, 2022, ss.214 - 219. 10.4274/meandros.galenos.2021.87004
AMA ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal. 2022; 23(2): 214 - 219. 10.4274/meandros.galenos.2021.87004
Vancouver ARSLAN Y,YENİSEY Ç Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray. Meandros Medical And Dental Journal. 2022; 23(2): 214 - 219. 10.4274/meandros.galenos.2021.87004
IEEE ARSLAN Y,YENİSEY Ç "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray." Meandros Medical And Dental Journal, 23, ss.214 - 219, 2022. 10.4274/meandros.galenos.2021.87004
ISNAD ARSLAN, Yasemin Tin - YENİSEY, Çiğdem. "Investigation of the Reparative and Regenerative Effects of Human Adipose Tissue Mesenchymal Stem Cells on Epidermal Cells Exposed to UVB Ray". Meandros Medical And Dental Journal 23/2 (2022), 214-219. https://doi.org/10.4274/meandros.galenos.2021.87004