TY  - JOUR
TI  - Investigation of the Reparative and
Regenerative Effects of Human Adipose
Tissue Mesenchymal Stem Cells on
Epidermal Cells Exposed to UVB Ray
AB  - Objective: To investigate the regenerative effects of adipose-derived mesenchymal
stem cells (ADMSCs) in immortalized human keratinocyte cells (HaCaT) exposed to
ultraviolet B (UVB) rays.
Materials and Methods: Epidermal cells were interacted with UVB rays. Then,
treatment was applied to ADMSC mediums prepared in different concentrations.
Cells that did not receive UVB light were included in the study as a control.
Results: With the application of human adipose tissue mesenchymal stem cells
to HaCaT cells with different concentrations of media prepared, cell viability
increased in both cells that received UVB radiation and those that did not. It has
been observed that the said increases are more pronounced, especially in cells that
have received UVB rays, reaching the maximum value at 100%, which is the highest
stem cell medium concentration. ADMSC medium also had an increasing effect
on the migration of UVB (+) cells. In UVB (+) cells, decreased levels of myeloid
cell leukemia 1 protein (Mcl-1) and phosphorylated extracellular signal-regulated
kinases (p-ERK 1/2) and dose-proportional increases were observed after treatment
with stem cell medium.
Conclusion: It has been determined that some factors in the ADMSC medium,
especially in keratinocytes that have received UVB rays, positively support
proliferation and show reparative and rejuvenating functions.
AU  - YENİSEY, Çiğdem
AU  - ARSLAN, Yasemin Tin
DO  - 10.4274/meandros.galenos.2021.87004
PY  - 2022
JO  - Meandros Medical And Dental Journal
VL  - 23
IS  - 2
SN  - 2149-9063
SP  - 214
EP  - 219
DB  - TRDizin
UR  - http://search/yayin/detay/534326
ER  -