Yıl: 2021 Cilt: 51 Sayı: 1 Sayfa Aralığı: 148 - 158 Metin Dili: İngilizce DOI: 10.3906/sag-2003-42 İndeks Tarihi: 26-07-2022

Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer

Öz:
Background/aim: Colorectal cancer (CRC) is a major public health problem worldwide and in Tunisia due to its increasing rate of incidence. KRAS and NRAS mutations have become a pivotal part of CRC diagnosis, given their association to treatment resistance with antiepidermal growth factor receptor (EGFR) monoclonal antibodies. In this study, we aimed to screen for mutations in KRAS and NRAS genes in Tunisian patients with CRC and explore their correlations with clinicopathological features. Materials and methods: AmoyDx KRAS and NRAS mutation real-time PCR kits were used to screen for mutations in KRAS (exon 2) and NRAS (exons 2, 3, and 4) in 96 CRC tumors. Results: KRAS exon 2 mutations were found in 41.7% (40/96) of the patients. Codon 12’s most abundant mutations were G12D and G12V, followed by G12A, while G13D is the predominant mutation in codon 13. KRAS exon 2 mutations were associated with older patients (P = 0.029), left-sided tumors (P = 0.037), and greater differentiation (P = 0.044). The prevalence rate of NRAS mutations was 7.3%, mostly in exon 2. These mutations were associated with early stages of the disease (P = 0.039) and the absence of lymph node metastasis (P = 0.045). Conclusion: It can be inferred from this study that Tunisian CRC patients have a similar frequency of KRAS and NRAS mutations compared to those observed in other populations. Consequently, screening for KRAS and NRAS mutations is crucial for the orientation of therapies and the selection of appropriate candidates, while also helping to avoid unnecessary toxicity and increased costs for patients.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA OUNISSI D, Weslati M, Boughriba R, HAZGUI M, bouraoui s (2021). Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. , 148 - 158. 10.3906/sag-2003-42
Chicago OUNISSI DONIA,Weslati Marwa,Boughriba Rahma,HAZGUI Meriam,bouraoui saadia Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. (2021): 148 - 158. 10.3906/sag-2003-42
MLA OUNISSI DONIA,Weslati Marwa,Boughriba Rahma,HAZGUI Meriam,bouraoui saadia Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. , 2021, ss.148 - 158. 10.3906/sag-2003-42
AMA OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. . 2021; 148 - 158. 10.3906/sag-2003-42
Vancouver OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. . 2021; 148 - 158. 10.3906/sag-2003-42
IEEE OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s "Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer." , ss.148 - 158, 2021. 10.3906/sag-2003-42
ISNAD OUNISSI, DONIA vd. "Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer". (2021), 148-158. https://doi.org/10.3906/sag-2003-42
APA OUNISSI D, Weslati M, Boughriba R, HAZGUI M, bouraoui s (2021). Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. Turkish Journal of Medical Sciences, 51(1), 148 - 158. 10.3906/sag-2003-42
Chicago OUNISSI DONIA,Weslati Marwa,Boughriba Rahma,HAZGUI Meriam,bouraoui saadia Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. Turkish Journal of Medical Sciences 51, no.1 (2021): 148 - 158. 10.3906/sag-2003-42
MLA OUNISSI DONIA,Weslati Marwa,Boughriba Rahma,HAZGUI Meriam,bouraoui saadia Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. Turkish Journal of Medical Sciences, vol.51, no.1, 2021, ss.148 - 158. 10.3906/sag-2003-42
AMA OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. Turkish Journal of Medical Sciences. 2021; 51(1): 148 - 158. 10.3906/sag-2003-42
Vancouver OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer. Turkish Journal of Medical Sciences. 2021; 51(1): 148 - 158. 10.3906/sag-2003-42
IEEE OUNISSI D,Weslati M,Boughriba R,HAZGUI M,bouraoui s "Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer." Turkish Journal of Medical Sciences, 51, ss.148 - 158, 2021. 10.3906/sag-2003-42
ISNAD OUNISSI, DONIA vd. "Clinicopathological characteristics and mutational profile of KRAS and NRAS in Tunisian patients with sporadic colorectal cancer". Turkish Journal of Medical Sciences 51/1 (2021), 148-158. https://doi.org/10.3906/sag-2003-42