TY  - JOUR
TI  - Medically important Candida spp. identification: an era beyond traditional methods
AB  - Background/aim: Candida infections are gaining more attention for the last few decades so diagnostic tools are very important for early
diagnosis. Conventional identification of yeasts is time-consuming, molecular methods are more complicated and relatively expensive
gold-standard methods. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) was put into
the market due to its speed and high accuracy. The aim of this study was to evaluate the performance of corn meal tween-80 agar
(CMTA), CHROMagar Candida medium, and MALDI-TOF MS and to compare the obtained results with DNA sequencing.
Materials and methods: The CHROMagar Candida medium, CMTA, and MALDI-TOF MS Biotyper System were used to test 416
isolates. The isolates with discrepant results by at least one of the three methods were subjected to sequence analysis.
Results: The identification results of the 351 (%84.4) were compatible with all three methods. When compared to the sequencing results,
the most accurate results were obtained by the MALDI-TOF MS, especially for rare Candida species.
Conclusion: MALDI-TOF MS is found to be the most accurate identification tool for clinically important Candida strains. CMTA
alone should not be used for the final identification of Candida species and the chromogenic medium should always be considered presumptive.
AU  - Yavuz, Yasemin
AU  - Karahan, Zeynep Ceren
AU  - Evren, Ebru
AU  - göçmen, julide sedef
AU  - TEKELI, ALPER
AU  - Hortaç İştar, Elvan
AU  - YAVUZDEMİR, ŞÜKRAN
DO  - 10.55730/1300-0144.5380
PY  - 2022
JO  - Turkish Journal of Medical Sciences
VL  - 52
IS  - 3
SN  - 1300-0144
SP  - 834
EP  - 840
DB  - TRDizin
UR  - http://search/yayin/detay/536644
ER  -