Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates

ÖZKAN, Melek; ÖZCENGİZ, Gülay

Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates

Melek ÖZKAN, (Gebze Yüksek Teknoloji Enstitüsü, Gebze, Türkiye)

Gülay ÖZCENGİZ (Orta Doğu Teknik Üniversitesi, Biyoloji Bölümü, Ankara)

Turkish Journal of Biology

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Yıl: 2006 Cilt: 30 Sayı: 1 Sayfa Aralığı: 45 - 50 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates

Öz:

Bu çalışmada üreme ve selüloz kullanım kapasiteleri bakımından bazı farklılıklar gösteren 13 ayrı termofilik, selülolitik, anaerobik, etanol üreticisi bakteri izolatının selülozomlarının CBM kodlayan bölgeleri PCR ile çoğaltılmış ve DNA dizileri saptanmıştır. Amplikonların kıyaslanan bölgelerinin DNA dizi analizleri, katı besiyerinde en yüksek selüloz kullanım kapasitesine sahip 7-9-1 suşunu da içeren 7 izolata ait CBM nükleotid ve amino asit dizilerinin Clostridium thermocellum'a ait CipB ile % 100 benzerliğe sahip olduklarını ortaya çıkarmıştır. Diğer yandan, yine yüksek selüloz kullanım kapasitesine sahip bir izolat olan 7-1-2 'ye ait CBM, 100 amino asitten 66'sında (%66) farklılık göstermiştir. Düşük selüloz kullanım kapasitesine sahip bir izolat olan 7-9-4 suşunda ise 118 amino asitten yalnızca 4'ünün farklı olduğu görülmüştür.

Anahtar Kelime: termofilik bakteriler yıkılma DNA dizi analizi karbonhidratlar selüloz seluloz sindirimi bağlanma bölgeleri Clostridium thermocellum selülolitik mikroorganizmalar polimeraz zincir reaksiyonu

Konular: Biyoloji

Çeşitli selülolitik, termofilik, anaerobik, etanol üreticisi izolatlarda karbonhidrat bağlanma modüllerinin birincil yapıları

Öz:

In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, was found to differ for as much as 66 amino acid residues out of 100 (66%). The isolate 7-9-4 with a relatively low cellulose-degrading capacity also displayed amino acid variation for this protein, but only for 4 out of 118 residues.

Anahtar Kelime: cellulose cellulose digestion binding sites Clostridium thermocellum cellulolytic microorganisms polymerase chain reaction thermophilic bacteria degradation DNA sequencing carbohydrates

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZKAN M, ÖZCENGİZ G (2006). Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. , 45 - 50.
Chicago	ÖZKAN Melek,ÖZCENGİZ Gülay Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. (2006): 45 - 50.
MLA	ÖZKAN Melek,ÖZCENGİZ Gülay Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. , 2006, ss.45 - 50.
AMA	ÖZKAN M,ÖZCENGİZ G Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. . 2006; 45 - 50.
Vancouver	ÖZKAN M,ÖZCENGİZ G Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. . 2006; 45 - 50.
IEEE	ÖZKAN M,ÖZCENGİZ G "Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates." , ss.45 - 50, 2006.
ISNAD	ÖZKAN, Melek - ÖZCENGİZ, Gülay. "Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates". (2006), 45-50.

APA	ÖZKAN M, ÖZCENGİZ G (2006). Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. Turkish Journal of Biology, 30(1), 45 - 50.
Chicago	ÖZKAN Melek,ÖZCENGİZ Gülay Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. Turkish Journal of Biology 30, no.1 (2006): 45 - 50.
MLA	ÖZKAN Melek,ÖZCENGİZ Gülay Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. Turkish Journal of Biology, vol.30, no.1, 2006, ss.45 - 50.
AMA	ÖZKAN M,ÖZCENGİZ G Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. Turkish Journal of Biology. 2006; 30(1): 45 - 50.
Vancouver	ÖZKAN M,ÖZCENGİZ G Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates. Turkish Journal of Biology. 2006; 30(1): 45 - 50.
IEEE	ÖZKAN M,ÖZCENGİZ G "Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates." Turkish Journal of Biology, 30, ss.45 - 50, 2006.
ISNAD	ÖZKAN, Melek - ÖZCENGİZ, Gülay. "Primary structure of the carbohydrate-binding modules in various cellulolytic, thermophilic, Anaerobic, Ethanol-Producing isolates". Turkish Journal of Biology 30/1 (2006), 45-50.