Yıl: 2004 Cilt: 17 Sayı: 3 Sayfa Aralığı: 25 - 33 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods

Öz:
Türkiye'de yetişen ve nikel metalini yaprakta biriktirme özelliği nedeni ile ekolojik değeri olan bazı Alyssum L. (Brassicaceae) türleri arasındaki genetik uzaklıklar RAPD-PCR ve SDS-PAGE yöntemleri ile belirlenmiştir. RAPD-PCR çalışmasında toplam 42 primer denenmiştir. Bunlardan beş primer polimorfik bantlar vermiş ve OPI18 primeri genetik uzaklığın tanımlanması için seçilmiştir. Genetik uzaklıklar hem RAPD-PCR hem de SDS-PAGE yöntemlerinde çalışılan türler için hesaplanmış ve SPSS bilgisayar programı ile dendrogramlar çıkartılmıştır. Alyssum cinsine ait türlerden SDS-PAGE yöntemi ile incelenenlerde türler arası polimorfizm, RAPD-PCR yöntemi ile incelenenlerde ise tür içi polimorfizm varlığı tespit edilmiştir.
Anahtar Kelime: SDS-PAGE Alyssum Brassicaceae polimorfizm genetik uzaklık genetik parametreler rastgele çoğaltılmış polimorfik DNA polimeraz zincir reaksiyonu

Türkiye' nin bazı Alyssum L. (Brassicaceae) türlerinin RAPD-PCR ve SDS- page yöntemleri ile moleküler analizleri

Öz:
RAPD-PCR and SDS-PAGE were used to investigate the pattern of genetic variation among some species of Alyssum L.(Brassicaceae); a nickel hyperaccumulator plant growing in Turkey. Forty two primers were used for the RAPD-PCR analyses. Polymorphic bands were obtained with five primers and OPI18 was selected for identifying the genetic distances. Genetic distances were calculated for all the species studied at both RAPD-PCR and SDS-PAGE methods, dendrograms were obtained with the SPSS computer program. Polymorphisms between Alyssum species and within species were determined by using SDS-PAGE and RAPD-PCR, respectively.
Anahtar Kelime: polymorphism genetic distance genetic parameters random amplified polymorphic DNA polymerase chain reaction SDS-PAGE Alyssum Brassicaceae

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA BABAOĞLU AYDAŞ S, AÇIK L, ÇELEBİ KESKİN A, ADIGÜZEL N (2004). Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. , 25 - 33.
Chicago BABAOĞLU AYDAŞ SENİHA SELCEN,AÇIK Leyla,ÇELEBİ KESKİN AYTEN,ADIGÜZEL Nezaket Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. (2004): 25 - 33.
MLA BABAOĞLU AYDAŞ SENİHA SELCEN,AÇIK Leyla,ÇELEBİ KESKİN AYTEN,ADIGÜZEL Nezaket Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. , 2004, ss.25 - 33.
AMA BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. . 2004; 25 - 33.
Vancouver BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. . 2004; 25 - 33.
IEEE BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N "Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods." , ss.25 - 33, 2004.
ISNAD BABAOĞLU AYDAŞ, SENİHA SELCEN vd. "Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods". (2004), 25-33.
APA BABAOĞLU AYDAŞ S, AÇIK L, ÇELEBİ KESKİN A, ADIGÜZEL N (2004). Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. Gazi Üniversitesi Fen Bilimleri Dergisi, 17(3), 25 - 33.
Chicago BABAOĞLU AYDAŞ SENİHA SELCEN,AÇIK Leyla,ÇELEBİ KESKİN AYTEN,ADIGÜZEL Nezaket Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. Gazi Üniversitesi Fen Bilimleri Dergisi 17, no.3 (2004): 25 - 33.
MLA BABAOĞLU AYDAŞ SENİHA SELCEN,AÇIK Leyla,ÇELEBİ KESKİN AYTEN,ADIGÜZEL Nezaket Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. Gazi Üniversitesi Fen Bilimleri Dergisi, vol.17, no.3, 2004, ss.25 - 33.
AMA BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. Gazi Üniversitesi Fen Bilimleri Dergisi. 2004; 17(3): 25 - 33.
Vancouver BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods. Gazi Üniversitesi Fen Bilimleri Dergisi. 2004; 17(3): 25 - 33.
IEEE BABAOĞLU AYDAŞ S,AÇIK L,ÇELEBİ KESKİN A,ADIGÜZEL N "Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods." Gazi Üniversitesi Fen Bilimleri Dergisi, 17, ss.25 - 33, 2004.
ISNAD BABAOĞLU AYDAŞ, SENİHA SELCEN vd. "Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by RAPD-PCR and SDS-page methods". Gazi Üniversitesi Fen Bilimleri Dergisi 17/3 (2004), 25-33.