Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)

Kilic, Hamdullah; OZER, Hakan; SÖKMEN, Münevver; Güllüce, Medine; BARIŞ, Özlem; ÖZBEK, Tülin; Sahin, Fikrettin; ÖZKAN, HAKAN

Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)

Özlem BARIŞ, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Erzurum, Türkiye)

Medine GÜLLÜCE, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Erzurum, Türkiye)

Fikrettin ŞAHİN, (Yeditepe Üniversitesi, Mühendislik Mimarlık Fakültesi, İstanbul, Türkiye)

Hakan ÖZER, (Atatürk Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Erzurum, Türkiye)

Hamdullah KILIÇ, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

Hakan ÖZKAN, (Erzurum Hıfzıssıhha Enstitüsü, Erzurum, Türkiye)

Münevver SÖKMEN, (Cumhuriyet Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Sivas, Türkiye)

Tülin ÖZBEK (Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Bölümü, Erzurum, Türkiye)

Turkish Journal of Biology

8 0

Yıl: 2006 Cilt: 30 Sayı: 2 Sayfa Aralığı: 65 - 73 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)

Öz:

Bu çalışmada, Achillea biebersteinii Afan. (Asteraceae)'nin metanol ekstraktı ve uçucu yağının laboratuar ortamında antimikrobiyal ve antioksidan aktiviteleri araştırıldı. Uçucu yağ 8 bakteri, 14 fungus ve C. albicans mayasına karşı antimikrobiyal aktivite gösterdi. Metanol ekstraktının ise antimikrobiyal aktivitesi gözlemlenemedi. Örneklerin antioksidan kapasitesi DPPH (2,2-diphenyl-l-picrylhydrazyl) ve (3-karoten/linoleik asit yöntemleri kullanılarak deıerlendirildi. Her iki yöntemde de ekstrakt, uçucu yaıdan daha iyi antioksidatif kapasite gösterdi. Ekstrakt serbest DPPH radikallerini uçucu yağdan (8900 ug/ml) daha düşük IC50 seviyesi (89.90 ug/ml) ile indirgedi. (3-karoten/linoleik asit yönteminde örnekler, linoleik oksidasyonunu etkili bir şekilde engelleyememiştir; 2 mg/ml'de BHT (%97.0)'ın çok altında kalarak, sırasıyla; sadece %22.7(ekstrakt) ve %16(uçucu yağ)'lık inhibisyon gösterdi. Ekstraktın toplam fenolik öğeleri, gallik asit eşdeıeri 51 ug/ml (% 5.1 w/w) olarak tespit edildi. Uçucu yağın GC-MS analizi %92.24'lük değerlendirme ile 64 bileşen tanılanarak sonuçlandı. Piperiton, kamfor ve 1,8-sineol (ökaliptol) uçucu yağın ana bileşenleri olarak belirlendi.

Anahtar Kelime: uçucu yağlar GC-MS antimikrobiyal özellikler metanol ökaliptol bitki özütü Achillea biebersteinii antioksidanlar kamfor

Konular: Biyoloji

Achillea biebersteinni afan.(Asteraceae)'nin uçucu yağı ve methanol ekstraktının biyolojik aktivitesi

Öz:

The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Man. (Asteraceae). The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. The antioxidative capacity of the samples was evaluated by using DPPH (2,2-diphenyl-l-picrylhydrazyl) and (3-carotene/linoleic acid assays. In both assays, the extract showed better antioxidative capacity than the oil. The extract reduced the stable free radical DPPH with lower ICS0 value (89.90 ug/ml) than the oil (8900 ug/ml). In the p-carotene/linoleic acid assay, the samples were not effectively able to inhibit the linoleic acid oxidation, exhibiting only 22.7% (the extract) and 16% (the essential oil) inhibitions at 2 mg/ml, far below than that of BHT (97.0%). Total phenolic constituent of the extract was 51 ug/mg (5.1%, w/w) as gallic acid equivalent. GC-MS analysis of the essential oil resulted in the identification of 64 components representing 92.24% of the oil. Piperitone, camphor and 1,8-cineole (eucalyptol) were the main constituents.

Anahtar Kelime: Achillea biebersteinii antioxidants camphor essential oils GC-MS antimicrobial properties methanol eucalyptol plant extracts

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BARIŞ Ö, Güllüce M, Sahin F, OZER H, Kilic H, ÖZKAN H, SÖKMEN M, ÖZBEK T (2006). Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). , 65 - 73.
Chicago	BARIŞ Özlem,Güllüce Medine,Sahin Fikrettin,OZER Hakan,Kilic Hamdullah,ÖZKAN HAKAN,SÖKMEN Münevver,ÖZBEK Tülin Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). (2006): 65 - 73.
MLA	BARIŞ Özlem,Güllüce Medine,Sahin Fikrettin,OZER Hakan,Kilic Hamdullah,ÖZKAN HAKAN,SÖKMEN Münevver,ÖZBEK Tülin Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). , 2006, ss.65 - 73.
AMA	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). . 2006; 65 - 73.
Vancouver	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). . 2006; 65 - 73.
IEEE	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T "Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)." , ss.65 - 73, 2006.
ISNAD	BARIŞ, Özlem vd. "Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)". (2006), 65-73.

APA	BARIŞ Ö, Güllüce M, Sahin F, OZER H, Kilic H, ÖZKAN H, SÖKMEN M, ÖZBEK T (2006). Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). Turkish Journal of Biology, 30(2), 65 - 73.
Chicago	BARIŞ Özlem,Güllüce Medine,Sahin Fikrettin,OZER Hakan,Kilic Hamdullah,ÖZKAN HAKAN,SÖKMEN Münevver,ÖZBEK Tülin Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). Turkish Journal of Biology 30, no.2 (2006): 65 - 73.
MLA	BARIŞ Özlem,Güllüce Medine,Sahin Fikrettin,OZER Hakan,Kilic Hamdullah,ÖZKAN HAKAN,SÖKMEN Münevver,ÖZBEK Tülin Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). Turkish Journal of Biology, vol.30, no.2, 2006, ss.65 - 73.
AMA	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). Turkish Journal of Biology. 2006; 30(2): 65 - 73.
Vancouver	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae). Turkish Journal of Biology. 2006; 30(2): 65 - 73.
IEEE	BARIŞ Ö,Güllüce M,Sahin F,OZER H,Kilic H,ÖZKAN H,SÖKMEN M,ÖZBEK T "Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)." Turkish Journal of Biology, 30, ss.65 - 73, 2006.
ISNAD	BARIŞ, Özlem vd. "Biological activities of the essential oil and methanol extract of achillea biebersteinii afan(Asteraceae)". Turkish Journal of Biology 30/2 (2006), 65-73.