Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity

KAHRAMAN, AHMET; Evcik, Fatma Deniz; KÖKEN, Tülay; Serteser, Mustafa

Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity

Mustafa SERTESER, (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Deniz EVCİK, (Kocatepe Üniversitesi, Tıp Fakültesi, Fiziksel Tıp ve Rehabilitasyon Anabilim Dalı, Afyonkarahisar, Türkiye)

Tülay KÖKEN, (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Ahmet KAHRAMAN (Afyon Kocatepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

Romatizma Dergisi

1 0

Yıl: 2006 Cilt: 21 Sayı: 1 Sayfa Aralığı: 18 - 22 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity

Öz:

Amaç: Reaktif Oksijen Ürünleri (ROS), romatoid artrit (RA) gibi akut ve kronik inflamatuvar olaylarda önemli rol oynamaktadırlar. Bu çalışma RA'li hastalardaki ROS ve antioksidan etkinin varlığını araştırmak amacıyla planlandı. Hastalar ve Yöntem: Antioksidan etkinlik total protein sülfidril (SH) düzeyi ile, ROS ise protein karbonil ve malondialdehid (MDA) düzeyleri ile belirlendi. Çalışmaya yaşları 31-68 arasında toplam 29 RA'li hasta alındı. Kontrol grubu aynı cins ve yaşta 20 sağlıklı bireyden oluşturuldu. Hasta ve kontrol grubunun serumlarından total protein SH, protein karbonil ve MDA düzeyleri ölçüldü. Bulgular: RA'li hastalarda total protein SH düzeylerinde kontrol grubuna göre belirgin azalma olduğu gözlendi (396.26±42.33 mmol/L vs 677.21±59.98 mmol/L, p<0.001). Ayrıca sırasıyla protein karbonil ve MDA düzeylerinde de kontrol grubuna göre istatistiksel anlamlı artış olduğu tespit edildi (105.45±14.26 mmol/L vs 93.65±10.49 mmol/L, p<0.05, 14.51±2.28 mmol/L vs 5.56±1.25 mmol/L, p<0.001). Hastalık aktivasyonu olanlarda remisyondakilere göre daha yüksek MDA düzeyleri ve daha düşük protein SH değerleri olduğu görüldü. RA'li hastalarda protein karbonil düzeyleri ve eritrosit sedimentasyon hızı (ESR) ve C-reaktif Protein (CRP) düzeyleri arasında negatif bir korelasyon (r=-0.415, p<0.05, r=-0.687, p<0.001) saptandı. Aktif hastalığı olanlarda herhangi bir ilişki bulunmamasına rağmen remisyondaki hastalarda protein karbonil ve ESR ve CRP arasında negatif bir korelasyon tespit edildi. Sonuç: Sonuç olarak RA'da ROS ‘de artış görülmektedir ve bu biyokimyasal belirteçlerin ölçümü ile inflamasyon düzeyi hakkında bilgi edinilebilinir.

Anahtar Kelime: Malondialdehit Reaktif oksijen türleri Artrit, romatoid Lipid peroksidasyonu

Konular: Romatoloji

Romatoid artrit'li hastaların, serum protein sülfidril, protein karbonil ve lipid peroksidasyon düzeylerindeki değişim: Hastalık aktivasyonu ile ilişkisi

Öz:

Objective: Reactive Oxygen Species (ROS) play a major role in the generation of acute and chronic inflammatory processes one of which is rheumatoid arthritis (RA). Patients and Methods: This study was designed to investigate ROS and antioxidant affects in RA patients. The antioxidant activity was determined by measuring total protein sulfhydryl (SH) levels. ROS was indicated by measuring protein carbonyls and malondialdehyde (MDA) levels. A total of 29 RA patients aged between 31 to 68 years old were recruited. Also 20 control subjects were selected from healthy individuals Total protein SH, protein carbonyls and MDA levels were measured from sera of both groups. Results: Total protein SH levels of RA patients were found to be significantly decreased than those found in control group (396,26±42,33 mmol/L vs 677,21±59,98 mmol/L, p<0,001). Also the levels of protein carbonyls and MDA were found to be statistically increased in RA patients respectively (105,45±14,26 mmol/L vs 93,65±10,49 mmol/L, p<0,05, 14,51±2,28 mmol/L vs 5,56±1,25 mmol/L, p<0,001). Patients active in disease had higher levels of MDA and lower levels of total protein SH levels when compared to those patients in remission. A negative correlation was found between protein carbonyls and erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) (r=-0,415, p<0,05, r=-0,687, p<0,001) in RA patients. No correlation was found in patients active in disease but negative corelation was found between protein carbonyls and ESR and CRP in patients in remission. Conclusion: In conclusion the level of ROS seems to increase and measurement of these markers may give us information about inflammatory process in RA.

Anahtar Kelime: Malondialdehyde Reactive Oxygen Species Arthritis, Rheumatoid Lipid Peroxidation

Konular: Romatoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Serteser M, Evcik F, KÖKEN T, KAHRAMAN A (2006). Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. , 18 - 22.
Chicago	Serteser Mustafa,Evcik Fatma Deniz,KÖKEN Tülay,KAHRAMAN AHMET Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. (2006): 18 - 22.
MLA	Serteser Mustafa,Evcik Fatma Deniz,KÖKEN Tülay,KAHRAMAN AHMET Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. , 2006, ss.18 - 22.
AMA	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. . 2006; 18 - 22.
Vancouver	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. . 2006; 18 - 22.
IEEE	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A "Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity." , ss.18 - 22, 2006.
ISNAD	Serteser, Mustafa vd. "Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity". (2006), 18-22.

APA	Serteser M, Evcik F, KÖKEN T, KAHRAMAN A (2006). Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. Romatizma Dergisi , 21(1), 18 - 22.
Chicago	Serteser Mustafa,Evcik Fatma Deniz,KÖKEN Tülay,KAHRAMAN AHMET Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. Romatizma Dergisi 21, no.1 (2006): 18 - 22.
MLA	Serteser Mustafa,Evcik Fatma Deniz,KÖKEN Tülay,KAHRAMAN AHMET Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. Romatizma Dergisi , vol.21, no.1, 2006, ss.18 - 22.
AMA	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. Romatizma Dergisi . 2006; 21(1): 18 - 22.
Vancouver	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity. Romatizma Dergisi . 2006; 21(1): 18 - 22.
IEEE	Serteser M,Evcik F,KÖKEN T,KAHRAMAN A "Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity." Romatizma Dergisi , 21, ss.18 - 22, 2006.
ISNAD	Serteser, Mustafa vd. "Changes in protein sulfhydryls, protein carbonyls and lipid peroxidation levels in sera of patients with rheumatoid arthritis: Correlation with disease activity". Romatizma Dergisi 21/1 (2006), 18-22.