Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey

KÖROĞLU, Ergün; ÜTÜK, Armağan Erdem; Simsek, Sami; ALTAY, KÜRSAT

Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey

Sami ŞİMŞEK, (Fırat Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Elazığ, Türkiye)

Ergün KÖROĞLU, (Fırat Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Elazığ, Türkiye)

Armağan Erdem ÜTÜK, (Fırat Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Elazığ, Türkiye)

Kürşat ALTAY (Fırat Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Elazığ, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2006 Cilt: 30 Sayı: 4 Sayfa Aralığı: 411 - 415 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey

Öz:

Bu çalışmanın amacı, Türkiye'nin doğusunda eozinofilik ve eozinofilik olmayan sığırlarda fasciolosisin araştırılmasıdır. Bu amaçla, çalışmaya dahil edilen sığırlarda Fasciola hepatica'ya karşı oluşmuş antikor yanıtı indirekt ES-ELISA ile araştırılmıştır. Absolut eozinofil sayımı yapılan 1200 sığırın 164 (% 13,6)'ü eozinofilik olarak bulunmuş ve bu 164 sığırın 121 (% 73,7)'inde indirekt ES-ELISA ile F. hepatica'ya karşı oluşmuş antikor cevabı tespit edilmiştir. Eozinofilik olmayan sığırlarda ise bu oran % 35 olarak belirlenmiştir (P < 0,001). Bu bulgular, özellikle fasciolosis gibi paraziter enfeksiyonların eozinofilinin önemli bir nedeni olduğuna ve sığır fasciolosisinin tanısında indirect ES-ELISA'nın kullanışlı bir test olduğuna işaret etmektedir.

Anahtar Kelime: bağışıksal tanı sığır teşhis eozinofili Fascioliasis Trematoda Türkiye antikor Fasciola hepatica antikor testleri tanı teknikleri ELISA

Konular: Ziraat Mühendisliği

Türkiye' nin doğusunda eozinofilik ve eozinofilik olmayan sığırlarda doğal Fasciola hepatica enfeksiyonunun tanısında indirekt excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA)' in kullanımı

Öz:

The aim of this study was to investigate fasciolosis in a group of eosinophilic and non-eosinophilic cattle in eastern Turkey. All the cattle were examined for antibodies against F. hepatica by modified indirect excretory/secretory enzyme-linked immunosorbent assay (ES-ELISA). Of the 1200 cattle tested, 164 (13.6%) were found to be eosinophilic by absolute eosinophil counts. Out of the 164 eosinophilic cattle, 121 (73.7%) were found to be positive for fasciolosis by indirect ES-ELISA, while the rate was only 35% among the non-eosinophilic group (P < 0.001). Our data indicate that parasitic infections should be considered an important cause of eosinophilia, particularly in fasciolosis, and the indirect ES-ELISA is useful as a screening test to examine anti-F. hepatica antibodies for the diagnosis of fasciolosis in cattle.

Anahtar Kelime: Turkey antibodies Fasciola hepatica antibody testing diagnostic techniques ELISA immunodiagnosis cattle diagnosis eosinophilia fascioliasis Trematoda

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1.Sanchez-Andrade, R., Paz-Silva, A., Suarez, J., Panadero, R., Diez-Banos, P., Morrado, P.: Use of a Sandwich-enzyme-linked immunosorbent assay (SEA) for the diagnosis of natural Fasciola hepatica infection in cattle from Galicia (NW Spain). Vet. Parasitol., 2000; 93: 39-46.
2.Hillyer, G.V.: Immunodiagnosis of human and animal fasciolosis, In: Dalton J.P., Ed. Fasciolosis. CABI Publishing, Oxon, Wallingford, UK, 1998; 435-448.
3.Bürger, H.J.: Helminthen. In: Körthing, W., Ed. Weterinarmedizinische Parasitologie. 4th Edition Verlag Paul Paney, Berlin, 1992; 174.
4.Happich, F.A., Boray, J.C.: Quantitive diagnosis of chronic fasciolosis. 1. Comparative studies on quantitative faecal examinations for chronic Fasciola hepatica infection in sheep. Aust. Vet. J., 1969; 45: 326-328.
5.Marin, M.S.: Epizootiologia de la fasciolosis bovina en Asturias. Identification y expresion de un antigeno unitario. PhD Thesis. Facultad de Biologia, Universitad de Oviedo. 1992.
6.Rothenberg, M.E.: Eosinophilia. N. Engl. J. Med., 1998; 338: 1592-1600.
7.Markel, E.K., John, D.T., Krotoski, W.A.: Immunodiagnostic techniques. In: Markel and Woge's Medical Parasitology (8th ed.). WB Saunders Company. Philadelphia, 1999; 473-480.
8.Lowry, O.H., Rosebrough, N.J., Farr, A.L, Randall, R.J.: Protein measurement with folin phenol reagent. J. Biol. Chem., 1951; 193: 265-275.
9.Swenson, M.J., Reece, W.O.: Dukes' physiology of domestic animals. Cornell University Press, eleventh edition, Ithaca and London. 1993.
10.Ibarra, F., Montenegro, N., Vera, Y., Boulard, C, Quiroz, H., Flores, J., Ochoa, P.: Comparison of three ELISA tests for seroepidemiology of ovine fasciolosis. Vet. Parasitol., 1998; 77: 229-236.
11.Reichel, M.P.: Performance characteristics of an enzyme-linked immunosorbent assay for the detection of liver fluke (Fasciola hepatica) infection in sheep and cattle. Vet. ParasitoH5,2002; 107: 65-72.
12.Şimşek, S., Köroğlu, E., Ütük, A.E., Altay, K.: Eozinofilik sığırlarda hydatidosis seropozitifliği. T. Parazitol. Derg., 2005; 29: 93-96.
13.Fagbemi, B.O., Obarisiagbon, I.O.: Comparative evaluation of the enzyme-linked immunosorbent assay (ELISA) in the diagnosis of natural Fasciola gigantica infection in cattle. Vet. Quart., 1990; 12:35-38.
14.Hillyer, G.V., Soler de Galanes, M.: Initial feasibility studies on the fast-ELISA for the immunodiagnosis of fascioliasis. J. Parasitol., 1991:77:362-365.
15.Gregorovic, V., Jazbec, I., Skusek, F.: Haematological and biochemical profiles of dairy cows in Slovenia. Dtsch. Tierarztl. Wochenschr., 1987; 94: 284-289.
16.Keller, H., Hosli, J., Eckert, J.: The clinical feature's and pathology of subacute fasciolosis in cattle. Tierarztl. Prax., 1998; 6: 187- 202.
17.Furmaga, S., Gundlach, J.I.: Behavior of morphotic blood elements and levels of iron and transferrin in the blood serum of calves experimentally infected with Fasciola hepatica (Trematoda). Acta Parasit. Polonica, 1978; 25: 179-189.
18.John, T.S., Park, B.J., Kang, S.W., Bak, U.B.: A pathological survey of liver lesions in slaughtered native cattle with special reference to fasciolosis. Korean J. Vet. Res., 1976; 16: 35-44.

APA	Simsek S, KÖROĞLU E, ÜTÜK A, ALTAY K (2006). Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. , 411 - 415.
Chicago	Simsek Sami,KÖROĞLU Ergün,ÜTÜK Armağan Erdem,ALTAY KÜRSAT Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. (2006): 411 - 415.
MLA	Simsek Sami,KÖROĞLU Ergün,ÜTÜK Armağan Erdem,ALTAY KÜRSAT Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. , 2006, ss.411 - 415.
AMA	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. . 2006; 411 - 415.
Vancouver	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. . 2006; 411 - 415.
IEEE	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K "Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey." , ss.411 - 415, 2006.
ISNAD	Simsek, Sami vd. "Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey". (2006), 411-415.

APA	Simsek S, KÖROĞLU E, ÜTÜK A, ALTAY K (2006). Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. Turkish Journal of Veterinary and Animal Sciences, 30(4), 411 - 415.
Chicago	Simsek Sami,KÖROĞLU Ergün,ÜTÜK Armağan Erdem,ALTAY KÜRSAT Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. Turkish Journal of Veterinary and Animal Sciences 30, no.4 (2006): 411 - 415.
MLA	Simsek Sami,KÖROĞLU Ergün,ÜTÜK Armağan Erdem,ALTAY KÜRSAT Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.30, no.4, 2006, ss.411 - 415.
AMA	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. Turkish Journal of Veterinary and Animal Sciences. 2006; 30(4): 411 - 415.
Vancouver	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey. Turkish Journal of Veterinary and Animal Sciences. 2006; 30(4): 411 - 415.
IEEE	Simsek S,KÖROĞLU E,ÜTÜK A,ALTAY K "Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey." Turkish Journal of Veterinary and Animal Sciences, 30, ss.411 - 415, 2006.
ISNAD	Simsek, Sami vd. "Use of indirect excretory/secretory Enzyme-Linked Immunosorbent Assay (ES-ELISA) for the diagnosis of natural Fasciola hepatica infection in eosinophilic and non-eosinophilic cattle from Eastern Turkey". Turkish Journal of Veterinary and Animal Sciences 30/4 (2006), 411-415.