Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi

Mehmet Cengiz BALOĞLU (Kastamonu Üniversitesi, Mühendislik ve Mimarlık Fakültesi, Genetik ve Biyomühendislik Bölümü, Kastamonu, Türkiye)
BEHCET İNAL (adres yazılmamıs)
3 8

Proje Grubu: KBAG Sayfa Sayısı: 1 Proje No: 215Z354 Proje Bitiş Tarihi: 01.04.2018 Metin Dili: Türkçe İndeks Tarihi: 07-12-2018

Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi

Öz:
Buğday, insan beslenmesinde kullanılan dünyada ve ülkemizde ekim ve üretim bakımından ilk sıralarda yer alan, en önemli tahıl ürünlerindendir. Buğdayın atalarından olan Triticum urartu ve Aegilops tauschii ile buğdayın modern kültürlerinden biri olan Triticum aestivum genomlarına ait dizilerin açığa çıkarılması, buğday genetik çalışmaları için bir dönüm noktası oluşturmaktadır. mikroRNA’ların (miRNA) bitkilerde stres koşullarına uyumda, patojenlerle etkileşimde, gelişim, üreme ve metabolizmada rol alan birçok yolakta rol aldığı kanıtlanmıştır. Kuraklık, hem ekmeklik hem de makarnalık un üretiminde kullanılan buğdayın kalitesini ve verimini önemli ölçüde etkilemektedir. Projemizde, farklı ploidi seviyesine sahip ve kuraklığa karşı farklı tolerans özellikleri gösteren Türk buğday çeşitlerinden; buğdayın atası olarak bilinen Siyez (T. monococcum) ile buğdayın modern kültürleri olan ekmeklik buğday olarak bilinen T. aestivum cv. Yüreğir-89 ve makarnalık buğday olarak bilinen T.turgidum durum cv. Kızıltan-91 buğday çeşitlerine ait kök ve yaprak dokularında kuraklık stresi sonucunda miRNA’ ların ve hedef genlerinin tespit edilmesi ve bunların ifade analizlerinin yapılması amaçlanmaktadır. Ayrıca, bu çalışma ile farklı ploidi seviyesine sahip buğday türlerinde kuraklık stresi ile ilişkili, önceden tanımlanmamış yeni miRNA’ ların bulunması hedeflenmektedir. Dolayısıyla, farklı kromozom setlerine sahip olan buğday türlerinde kuraklık stresi sonucu hangi miRNA’ ların görev yaptığı ve ifade seviyelerindeki değişiklikler belirlenerek, miRNA’ ların orijinleri, evrimsel açıdan değerlendirilmiştir. Bununla birlikte, çalışılan buğday çeşitleri kuraklık stresine farklı cevap oluşturduğu bilinen farklı ploidi seviyesine sahip buğday çeşitleri olduğu için, tanımlanmış ve henüz tanımlanmamış yeni miRNA’ ların kuraklığa hassas ve dayanıklı bireylerdeki durumları, bu miRNA’ ların hangi buğday türünden köken aldıkları da ilk kez bu çalışma ile incelenmiştir. Ayrıca, belirlenen miRNA’ ların hedef genleri hem biyoinformatik yöntemeler hem de degradom kütüphaneleri yardımıyla kuraklığa farklı cevap veren buğday türlerinde tespit edilmiş ve bu hedef genlerin Gene Ontology, MapMan yolak analizleri yapılarak gen ifade analizleri Kantitatif Real-Time PCR ile kontrol edilmiştir. Bunlara ilaveten, kök ve yaprak dokularında miRNA ve hedef genlerinin kuraklığa vereceği cevapların incelenmiş olması, bu proje ile ilk defa farklı ploidi ve kuraklık toleransına sahip buğday çeşitlerinde farklı dokularda kuraklık stresinin miRNA seviyesinde analizi yapılmıştır. Daha önceden buğdaya ait miRNA’ lar belirlenmiş olsa da, farklı ploidi seviyelerindeki buğday türlerinde kuraklık stresi altında önceden tanımlanmış miRNA’ ların tespit edilmesi, tanımlanmamış yeni miRNA’ ların bulunması, bunların hedef genlerinin belirlenmesi ve ifade seviyelerinin incelenmesi hakkında literatürde bilgi eksikliği mevcuttur. Bu sebeple yapılan bu çalışma farklı kromozom setlerine sahip, kuraklığa karşı farklı tolerans özellikleri gösteren Türk buğday çeşitlerinde, kuraklık stresi ile ilgili moleküler mekanizmaların aydınlatılması ile ilgili verilerin ortaya çıkmasını sağlamıştır.
Anahtar Kelime: Kantitatif Real-Time PCR Biyoinformatik Hedef Genler miRNA Kuraklık Stresi Ploidi Buğday

Konular: Genetik ve Kalıtım Gıda Bilimi ve Teknolojisi Ziraat, Toprak Bilimi

Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi

Öz:
Wheat, used in the human diet and placed in the first in terms of cultivation and production areas in the world and in our country, is the most important grain crops. Elucidating the sequences of genomes of wheat species including ancestors of Triticum urartu and Aegilops tauschii and modern culture of the wheat Triticum aestivum is a turning point for wheat genetic studies. microRNAs (miRNAs) in adaptation to stress conditions in plants, pathogens interaction, development, reproduction, and metabolism is implicated in proven to be involved in many pathways. Drought considerably affects the quality and yield of flour used in both bread and pasta production. In this project; it is aimed to determine miRNA’s and their target genes and perform their expression analysis after drought stress treatment in different ploidy levels and different drought tolerance characteristics of the Turkish wheat varieties including Siyez known as the ancestor of wheat (T. monococcum), T. aestivum cv. Yüreğir-89 known as the modern culture of the wheat bread and T. turgidum durum cv. Kızıltan-91 known as durum wheat. In addition, this study aims identification of previously unidentified novel miRNA’s associated with drought stress in root and leaf tissues of wheat species with different ploidy level. Therefore, it was determined that; as a result of drought stress which miRNA’s was served and how their expression levels were changed in wheat cultivars having different types of chromosome pairs. miRNA’s origins and phylogenetic distributions was also considered from an evolutionary perspective. In addition, wheat varieties to be studied are known to be the different response to drought stress conditions and different ploidy levels, defined and unidentified novel miRNA 's which was identified in this study have been also investigated in sensitive and resistant individuals. Origin of these miRNA's and which miRNAs arose from wheat cultivars was examined at the first time by this study. Besides, miRNA`s target genes was identified using both bioinformatics methods and degradome libraries in wheat varieties that respond to drought stress differently. Gene Ontology and MapMan pathway analyzes of these target genes was performed and gene expression analysis was controlled by Quantitative Real-Time PCR. Although wheat miRNA’s have been previously determined, there is a lack of knowledge in the literature about detection of defined, unidentified novel miRNA` s and their target genes in wheat varieties with different ploidy levels under drought stress and examination of their expression levels. Therefore, this study allowed the opening of new areas related to identification molecular mechanisms of drought stress in the Turkish wheat varieties showing different drought tolerance characteristics with different pairs of chromosomes.
Anahtar Kelime:

Konular: Genetik ve Kalıtım Gıda Bilimi ve Teknolojisi Ziraat, Toprak Bilimi
Erişim Türü: Erişime Açık
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APA BALOĞLU M, İNAL B (2018). Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. , 0 - 1.
Chicago BALOĞLU Mehmet Cengiz,İNAL BEHCET Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. (2018): 0 - 1.
MLA BALOĞLU Mehmet Cengiz,İNAL BEHCET Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. , 2018, ss.0 - 1.
AMA BALOĞLU M,İNAL B Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. . 2018; 0 - 1.
Vancouver BALOĞLU M,İNAL B Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. . 2018; 0 - 1.
IEEE BALOĞLU M,İNAL B "Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi." , ss.0 - 1, 2018.
ISNAD BALOĞLU, Mehmet Cengiz - İNAL, BEHCET. "Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi". (2018), 0-1.
APA BALOĞLU M, İNAL B (2018). Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. , 0 - 1.
Chicago BALOĞLU Mehmet Cengiz,İNAL BEHCET Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. (2018): 0 - 1.
MLA BALOĞLU Mehmet Cengiz,İNAL BEHCET Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. , 2018, ss.0 - 1.
AMA BALOĞLU M,İNAL B Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. . 2018; 0 - 1.
Vancouver BALOĞLU M,İNAL B Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi. . 2018; 0 - 1.
IEEE BALOĞLU M,İNAL B "Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi." , ss.0 - 1, 2018.
ISNAD BALOĞLU, Mehmet Cengiz - İNAL, BEHCET. "Farklı Ploidi Seviyelerine Sahip Türk Buğday Çeşitlerinde Kuraklık Stresi Altında mikroRNA’ ların Derinleme Dizilenmesi ve Hedef Genlerin Analizi". (2018), 0-1.
Sayfa Oluşturulma Tarihi
24-04-2024 1:31

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