Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols

Karakaya, Sibel; El, Sedef Nehir

Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols

Sibel KARAKAYA, (Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, İzmir, Türkiye)

Sedef Nehir EL (Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, İzmir, Türkiye)

Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi

7 3

Yıl: 2006 Cilt: 23 Sayı: 1 Sayfa Aralığı: 1 - 8 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols

Öz:

Son yıllarda fenolik bileşikler ve gıdaların antioksidan aktiviteleri üzerine yapılan çalışmaların sayısındaki artış, polifenollerce zengin gıdaların tüketimi ile dejeneratif hastalıklar arasında negatif ilişkinin saptanmasına bağlanmaktadır. Bu ilişkinin in vivo koşullarda sağlanıp sağlanamayacağını açıklığa kavuşturmak için fenolik bileşiklerin biyoyararlılığına ait çalışmalara gereksinim olduğu belirtilmektedir. Bu çalışmanın amacı Akdeniz Bölgesinde popüler olarak tüketilen adaçayı, ıhlamur, ısırgan otu, kurutulmuş ısırgan out ve siyah çayın toplam fenolik madde konsantrasyonları ile antioksidan aktivitelerini saptamak ve standart fenolik bileşikler (kateşin, ferulik asit, kuersetin) ile Troloksun antioksidan aktiviteleriyle karşılaştırmaktır. Çalışmanın bir diğer amacı ise siyah çaydaki fenolik bileşiklerin in vitro biyoyararlılığının belirlenmesidir. Siyah çay ve siyah çay dializatının ABTS radikal oksidasyonu üzerine inhibisyonları sırasıyla % 99.43 ve % 42 olarak saptanmıştır. Adaçayı, ıhlamur, ısırgan otu ve kurutulmuş ısırgan otu ile elde edilen çaylar ise ABTS radikal oksidasyonunu sırsıyla %39.61, % 96.70, %70.80 ve %95.50 düzeylerinde inhibe etmişlerdir. Siyah çay dializatının toplam fenolik madde içeriğindeki azalma % 96.48 olmasına rağmen toplam antioksidan aktivitedeki azalma % 57.76 olarak saptanmıştır. On uM kateşin ve 10 uM kuersetin ABTS radikal oksidasyonunu %100 inhibe etmiş, oysa Troloks ve ferulik asit için aynı inhibisyon 20 |j,M konsantrasyonda saptanmıştır. Bir fincan çayın toplam antioksidan aktivitesi 10fiM kuersetin, IOuM kateşin, 20 uM Troloks ve 20 uM ferulik aside eşdeğer bulunmuştur. Siyah çay dializatının toplam antioksidan aktivitesi ise 5 uM Troloks ve 5 uM ferulik asitten biraz düşük olmuştur.

Anahtar Kelime: Urtica dioica sağlık biyoyararlanım ıhlamur adaçayı bitki çayları fenoller ısırgan otu Urtica urens antioksidanlar çay

Konular: Ziraat Mühendisliği

Batı bitkisel çayların toplam fenolik madde içerikleri, antioksidan aktiviteleri ve siyah çay polifenollerinin in vitro biyoyararlılığı

Öz:

In recent years, studies on phenolic compounds and antioxidant activities of foods have increased due to the inverse relationship between degenerative diseases and consumption of polyphenol rich foods. To clarify the controversy related to if antioxidant activity can be supplied in vivo, the necessity for data on bioavailability of phenolic compounds are considered. The aim of the study was to determine total phenols and antioxidant activities of sage, linden flower, fresh nettle, dried nettle leaves and black tea infusions which are popular beverages in the Mediterranean region and also to evaluate antioxidant activities of standard phenolic compounds (catechin, ferulic acid, quercetin) and Trolox for comparison. Moreover, another goal was to determine in vitro bioavailability of black tea. Both black tea infusion and black tea dialysate inhibited ABTS radical cation oxidation by 99.43% and 42% respectively. Infusions of sage, linden flower, fresh nettle, dried nettle leaves inhibited ABTS radical cation oxidation by 39.61%, 96.70%, 70.80% and 95.50% respectively. Although total phenols of black tea dialysate decreased by 96.48%, total antioxidant activity decreased by 57.76%. Ten uM catechin and lOuM quercetin showed 100% inhibition on ABTS radical cation oxidation. However, Trolox and ferulic acid showed 100% inhibition at 20 uM concentrations. Total antioxidant activity of a cup of black tea was found to be equal to 10 uM of quercetin, 10 uM of catechin, 20 uM of Trolox and 20 uM of ferulic acid. Total antioxidant activity of black tea dialysate was slightly higher than both 5 jxM of Trolox and 5 uM of ferulic acid.

Anahtar Kelime: phenols nettle Urtica urens antioxidants tea Urtica dioica health bioavailability linden sage herbal teas

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Karakaya S, El S (2006). Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. , 1 - 8.
Chicago	Karakaya Sibel,El Sedef Nehir Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. (2006): 1 - 8.
MLA	Karakaya Sibel,El Sedef Nehir Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. , 2006, ss.1 - 8.
AMA	Karakaya S,El S Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. . 2006; 1 - 8.
Vancouver	Karakaya S,El S Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. . 2006; 1 - 8.
IEEE	Karakaya S,El S "Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols." , ss.1 - 8, 2006.
ISNAD	Karakaya, Sibel - El, Sedef Nehir. "Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols". (2006), 1-8.

APA	Karakaya S, El S (2006). Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, 23(1), 1 - 8.
Chicago	Karakaya Sibel,El Sedef Nehir Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi 23, no.1 (2006): 1 - 8.
MLA	Karakaya Sibel,El Sedef Nehir Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, vol.23, no.1, 2006, ss.1 - 8.
AMA	Karakaya S,El S Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi. 2006; 23(1): 1 - 8.
Vancouver	Karakaya S,El S Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi. 2006; 23(1): 1 - 8.
IEEE	Karakaya S,El S "Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols." Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, 23, ss.1 - 8, 2006.
ISNAD	Karakaya, Sibel - El, Sedef Nehir. "Total phenols and antioxidant activities of some herbal teas and In vitro biovailability of black tea polypenols". Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi 23/1 (2006), 1-8.