Yıl: 2006 Cilt: 19 Sayı: 2 Sayfa Aralığı: 229 - 234 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

A direct RP-HPLC determination of phenolic compounds in Turkish red wine

Öz:
Dört farklı Türk üzüm çeşidinden yapılan kırmızı şaraplar, fenolik içeriklerini belirlemek amacıyla analiz edilmişlerdir. Analiz için diode array detectör eşliğinde ters fazlı-yüksek basınçlı sıvı kromotografisi (RP-HPLC) kullanılmıştır. Türkiye’de yetiştirilen Kalecik karası, Öküzgözü, Boğazkere ve Papazkarası üzüm çeşitlerinden elde edilen kırmızı şaraplar arasındaki karakteristik farkları belirlemek için, sekiz fenolik asit (ferulik, o-kumarik, p-kumarik, kaffeik, siyringik, trans-sinnamik, klorogenik ve gallik asitler) ile beş flavonoid ((+)-kateşin, (-)-epikateşin, kuersetin, vanillin ve rutin) standartı kullanılmıştır. Şarap örneklerinde en fazla bulunan fenolikler, flavonoidlerden (+)-kateşin (17,82–33,59 mg L-1) ve fenolik asitlerden de gallik asit (13,25-16,39 mg L-1) olurken, klorogenik asit hiçbir şarap örneğinde belirlenememiştir. Sonuç olarak, fenolik maddelerin tip ve konsantarsyonlarının farklı çeşitlerden elde edilen şaraplara göre değiştiği belirlenmiştir.
Anahtar Kelime: flavonoids Turkey alcoholic beverages wines HPLC phenolic compounds techniques red wines chemical composition liquid chromatography

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Abril, M., Negueruela, A.I., Pérez, C., Juan, T. and Estopanan, G., 2005. Preliminary Study of Resveratrol Content in Aragón Red and Rosé Wines. Food Chemistry, 92(4): 729-736.
  • Andrade, P. B., Mendes, Falco, G.V., Valentão, P. and Seabra, R. M., 2001. Preliminary Study of Flavonols in Port Wine Grape Varieties Food Chemistry, : 397-399.
  • Bayhan, A., 2004. Determination of some phenolic compounds by using GC-MS methods in wines produced from different kalecik karası clones. Ankara University, p 37, Master Thesis.
  • Briviba, K. and Sies, H., 1994. Nonenzymathic Antioxidant Defence Systems. In: B. Frei (Editor), Natural Antioxidant in Human Health and Disease. Academic Press Inc., New York, USA, pp. 107-128.
  • Caponio, F., Alloggio, V. and Gomes, T., 1999. Phenolic Compounds of Virgin Olive Oil:Influence of Paste Preparation Techniques. Food Chemistry, 64: 203-209.
  • Castellari M., Sartini E., Fabiani A., Arfelli G. and Amati A., 2002. Analysis of Wine Phenolics by High Performance Liquid Chromatography Using a Monolithic Type Column. Journal of Chromatography A, 973 (1-2): 221-227.
  • De Villiers A., Vanhoenacker G., Majek P. and Sandra P., 2004. Determination of Anthocyanins in Wine by Direct Injection Liquid Chromatography-Diode Array Detection-Mass Spectrometry and Classification of Wines Using Discriminant Analysis. Journal of Chromatography A, 1054 (1-2): 195-204.
  • Gambelli, L. and Santaroni, G. P., 2004. Polyphenols Content in Some Italian Red Wines of Different Geographical Origins. Journal of Food Composition and Analysis, 17(5): 613-618.
  • Goldberg, D.M., Karumanchiri, A., Tsang, E., and Soleas, G.J., 1998. Catechin and epicatechin concentrations of red wines: Regional and cultivar-related differences. American Journal of Enology and Viticulture, 49: 23-34.
  • Huang, M., Ho, C. and Lee, C.,1992. Phenolic Compounds in Foods and Their Effects on
  • Health. American Chemical Society, Washington, USA.
  • Husain, S.R., Cillart, J. and Cillart, P., 1987. Hydroxy Radical Scavenging Activity of Flavonoids. Phytochemistry, 26 (9): 2489-2491.
  • Ibern-Gomez M., Andres-Lacueva C., Lamuela- Raventos R.M. and Waterhouse A.L., 2002. Rapid HPLC Analysis of Phenolic Compounds in Red Wines. American Journal of Enology And Viticulture, 53 (3): 218-221.
  • Kallithraka, S., Arvanitoyannis, I., El-Zajouli, A. and Kefalas, P., 2001. The Application of an Improved Method for Trans-Resveratrol to Determine the Origin of Greek Red Wines. Food Chremistry, 75: 355-363.
  • Kılınç, E. and Kalkan, H., 2003. High-performance Liquid Chromatographic Determination of Some Phenolic Acids of Turkish Commercial Wines: An Electrochemical Approach Journal of Wine Research, 14 (1): 17–23.
  • Kolouchova-Hanzlíkova, H., Melzoch, K., Filip, V. and Smidrkal J., 2004. Rapid Method for Resveratrol Determination by HPLC with Electrochemical and UV Detections in Wines. Food Chemistry, 87(1): 151-158.
  • Lopez, M., Martinez, F., Del Valle, C. and Miro, M., 2001. Analyses of Phenolic Consistents of Biological Interest in Red Wines by High Performance Liquid Chromatography. Journal of Chromatography A, 922: 359-363.
  • Lopez-Velez, M., Martinez, F. and Del Valle, C., 2003. The Study of Phenolic Compouınds as Natural Antioxidants in Wine. Critical Reviews in Food Science And Nutrition, 43 (3): 233-244.
  • Oszmianski, J. and Lee, C.Y., 1990. Isolation and HPLC Determination of Phenolic Compounds in Red Grapes. American Journal of Enology and Viticulture, 41 (3): 204-206.
  • Proestos, C., Bakogiannis, A., Psarianos, C., Koutinas, A.A., Kanellaki, M. and Komaitis, M., 2005. High Performance Liquid Chromatography Analyses of Phenolic Substances in Greek. Food Control, 16: 319-323.
  • Revilla, E. and Ryan, J.M., 2000. Analyses of Several Phenolic Compounds with Potential Antioxidant Properties in Grape Extracts and Wines by High Performance Liquid Chromatography- Photodiode Array Detection without Sample Preperation. Journal of Chromatography A, 881: 461-469.
  • Rice-Evans, C. and Packer, L., 1998. Flavonoids in Health And Disease. Marcel Dekker, New York, USA.
  • Rodriguez-Delgado, M.A., Malovana, S., Perez, J.P., Borges, T. and Garcia Montelongo, F.J., 2001. Seperation of Phenolic Compounds by High Performence Liquid Chromatography with Absorbance and Fluoritmetric Detection. Journal of Chromatography A, 912: 249-257.
  • Rosa, M., Raventos, L. and Waterhouse, A.L., 1994. A Direct HPLC Separation of Wine Phenolics. American Journal of Enology and Viticulture, 45(1): 1-5.
  • Rubichaud, J.L. and Noble, A.C., 1990. Astringency and Bitterness of Selected Phenolics in Wine. Journal of the Science of Food and Agriculture, 53: 343-353.
  • Suarez B., Palacios N., Fraga N. and Rodriguez R., 2005. Liquid Chromatographic Method for Quantifying Polyphenols iIn Ciders by Direct Injection. Journal of Chromatography A, 1066 (1-2): 105-110.
  • Tsanova-Savova, S., Dimov, S. and Ribarova, F., 2002. Anthocyanins and Color Variables of Bulgarian Aged Red Wines. Journal of Food Composition and Analysis, 15 (6): 647-654.
  • Villano, D., Fernandez-Pachon, M.S., Troncoso, A.M. and Garcia-Parrilla, M.C., 2005. Comparison of Antioxidant Activity of Wine Phenolic Compounds and Metabolites In Vitro. Analytica Chimica Acta, 538 (1-2): 391-398.
  • Zhou, J., Cui, H., Wan, G., Xu, H., Pang, Y. and Duan, C., 2004. Direct Analysis of Trans- Resveratrol in Red Wine by High Performance Liquid Chromatography with Chemiluminescent Detection .Food Chemistry, 88 (4): 613-620.
APA ÖZKAN G, BAYDAR G (2006). A direct RP-HPLC determination of phenolic compounds in Turkish red wine. , 229 - 234.
Chicago ÖZKAN Gülcan,BAYDAR Göktürk Nilgün A direct RP-HPLC determination of phenolic compounds in Turkish red wine. (2006): 229 - 234.
MLA ÖZKAN Gülcan,BAYDAR Göktürk Nilgün A direct RP-HPLC determination of phenolic compounds in Turkish red wine. , 2006, ss.229 - 234.
AMA ÖZKAN G,BAYDAR G A direct RP-HPLC determination of phenolic compounds in Turkish red wine. . 2006; 229 - 234.
Vancouver ÖZKAN G,BAYDAR G A direct RP-HPLC determination of phenolic compounds in Turkish red wine. . 2006; 229 - 234.
IEEE ÖZKAN G,BAYDAR G "A direct RP-HPLC determination of phenolic compounds in Turkish red wine." , ss.229 - 234, 2006.
ISNAD ÖZKAN, Gülcan - BAYDAR, Göktürk Nilgün. "A direct RP-HPLC determination of phenolic compounds in Turkish red wine". (2006), 229-234.
APA ÖZKAN G, BAYDAR G (2006). A direct RP-HPLC determination of phenolic compounds in Turkish red wine. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 19(2), 229 - 234.
Chicago ÖZKAN Gülcan,BAYDAR Göktürk Nilgün A direct RP-HPLC determination of phenolic compounds in Turkish red wine. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 19, no.2 (2006): 229 - 234.
MLA ÖZKAN Gülcan,BAYDAR Göktürk Nilgün A direct RP-HPLC determination of phenolic compounds in Turkish red wine. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, vol.19, no.2, 2006, ss.229 - 234.
AMA ÖZKAN G,BAYDAR G A direct RP-HPLC determination of phenolic compounds in Turkish red wine. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi. 2006; 19(2): 229 - 234.
Vancouver ÖZKAN G,BAYDAR G A direct RP-HPLC determination of phenolic compounds in Turkish red wine. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi. 2006; 19(2): 229 - 234.
IEEE ÖZKAN G,BAYDAR G "A direct RP-HPLC determination of phenolic compounds in Turkish red wine." Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 19, ss.229 - 234, 2006.
ISNAD ÖZKAN, Gülcan - BAYDAR, Göktürk Nilgün. "A direct RP-HPLC determination of phenolic compounds in Turkish red wine". Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 19/2 (2006), 229-234.