In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme

ÖZDEMİR, Hasan; ÇANKAYA, MURAT; YÖRÜK, Özgür; ŞİŞECİOĞLU, MELDA

In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme

Murat ÇANKAYA, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Kimya Anabilim Dalı, Erzurum, Türkiye)

Melda ŞİŞECİOĞLU, (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Kimya Anabilim Dalı, Erzurum, Türkiye)

Özgür YÖRÜK, (Atatürk Üniversitesi, Tıp Fakültesi, Otolaringoloji Anabilim Dalı, Erzurum, Türkiye)

Hasan ÖZDEMİR (Atatürk Üniversitesi, Fen Edebiyat Fakültesi, Kimya Anabilim Dalı, Erzurum, Türkiye)

Turkish Journal of Medical Sciences

3 0

Yıl: 2006 Cilt: 36 Sayı: 5 Sayfa Aralığı: 301 - 306 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme

Öz:

Amaç: Doğum öncesi ve sonrası insan ve hayvanlarda antibiyotiklerin yaygın kullanılması sebebiyle, emzirme esnasında bu antibiyotiklerin laktoperoksidaz enzimi aktivitesi üzerine etkisi olduğu düşünülebilir. Bu amaçla gentamicine sulfate ve ampiciline sodium ilaçlarının sığır laktoperoksidaz enzimi aktivitesi üzerine etkisi incelendi. Yöntem ve Gereç: Laktoperoksidaz enzimi Amberlite reçinesi, Sephadex G-100 jel filitrasyon ve CM Sephadex iyon değişim kromatografisi kullanılarak yağı alınmış sığır sütünden saflaştırıldı. Saflaştırılmış süttee Rz(A412/A280) saflaşma değeri 0,8 bulundu. Enzimi aktivitesi, ve inhibisyon çalışmalarında substrat olarak pH 6 da 2,21-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) (ABTS) kullanıldı. Bulgular: Saflaştırılmış enzimin SDS-PAGE elektroferezi yapıldığında tek bant bulundu. Gentamicine sulphate ve ampiciline sodium için yüzde aktivite grafikleri çizilerek I 50 değerleri, hesaplandı. Bu garfiklerden gentamicine sulfatın I 50 değeri 3,71mM bulunurken ampiciline sodium I 50 değeri 0,89 mM olarak hesaplandı. Lineweaver-Burk grafikleri çizilerek her iki ilaç için Ki değerleri tespit edilmiş, gentamicine sulfatın Ki değeri 4,36 ± 3.122 mM, ampiciline sodiumun Ki değeri 0.473 ± 0.099 mM olarak hesaplanmıştır. Sonuç: Antibiyotik kullanımı emzirme esnasıda sütte bulunan laktoperoksidaz enziminin aktivitesini düşürebilir. Bu antibiyotikleri emzirme esnasında kullanmak gerekirse yan etkileri en aza indirmek için verilecek miktarları hesaplanarak ayarlanabilir.

Anahtar Kelime: Elektroforez, poliakrilamid jel Anti-bakteriyel ajanlar Laktoperoksidaz Gentamisinler Enzim aktivasyonu Laktasyon Ampisilin

Konular: Cerrahi

Sığır laktoperoksidaz enzimi üzerine bazı antibiyotiklerin etkisi

Öz:

Aim: Owing to the widespread use of antibiotic drugs in both human and animals, we thought it would be important to determine the effect of these on lactoperoxidase (LPO; E.C. 1.11.1.7) activity during lactation. The in vitro effects of gentamicin sulfate and ampicillin sodium drugs on bovine LPO enzyme activity in breastmilk were investigated. Materials and Methods: LPO was purified with Amberlite CG 50 resin, CM Sephadex C-50 ion-exchange chromatography, and Sephadex G-100 gel filtration chromatography from skimmed bovine milk. Rz(A412/A280) value for the purified LPO was found to be 0.8. Inhibition or activation effects of the drugs on LPO enzyme were determined using 2,21-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate at pH = 6.0. Results: The purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). I50 values of gentamicin sulfate and ampicillin sodium drugs exhibiting inhibition effects were obtained by drawing % Activity- [drugs] graphs. The obtained I50 values of gentamicin sulfate and ampicillin sodium were 3.71 mM and 0.89 mM, respectively. Ki constants of the drugs were found by means of Lineweaver-Burk graphs. The obtained Ki constants for gentamicin sulfate and ampicillin sodium were 4.36 ± 3.122 mM and 0.473 ± 0.099 mM, respectively, and they were competitive inhibitors. Conclusions: Use of antibiotics may worsen the LPO activity in milk during lactation. If these antibiotic drugs are required during lactation, their dosage should be carefully determined to decrease the side effects.

Anahtar Kelime: Ampicillin Electrophoresis, Polyacrylamide Gel Anti-Bacterial Agents Lactoperoxidase Gentamicins Enzyme Activation Lactation

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÇANKAYA M, ŞİŞECİOĞLU M, YÖRÜK Ö, ÖZDEMİR H (2006). In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. , 301 - 306.
Chicago	ÇANKAYA MURAT,ŞİŞECİOĞLU MELDA,YÖRÜK Özgür,ÖZDEMİR Hasan In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. (2006): 301 - 306.
MLA	ÇANKAYA MURAT,ŞİŞECİOĞLU MELDA,YÖRÜK Özgür,ÖZDEMİR Hasan In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. , 2006, ss.301 - 306.
AMA	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. . 2006; 301 - 306.
Vancouver	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. . 2006; 301 - 306.
IEEE	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H "In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme." , ss.301 - 306, 2006.
ISNAD	ÇANKAYA, MURAT vd. "In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme". (2006), 301-306.

APA	ÇANKAYA M, ŞİŞECİOĞLU M, YÖRÜK Ö, ÖZDEMİR H (2006). In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. Turkish Journal of Medical Sciences, 36(5), 301 - 306.
Chicago	ÇANKAYA MURAT,ŞİŞECİOĞLU MELDA,YÖRÜK Özgür,ÖZDEMİR Hasan In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. Turkish Journal of Medical Sciences 36, no.5 (2006): 301 - 306.
MLA	ÇANKAYA MURAT,ŞİŞECİOĞLU MELDA,YÖRÜK Özgür,ÖZDEMİR Hasan In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. Turkish Journal of Medical Sciences, vol.36, no.5, 2006, ss.301 - 306.
AMA	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. Turkish Journal of Medical Sciences. 2006; 36(5): 301 - 306.
Vancouver	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme. Turkish Journal of Medical Sciences. 2006; 36(5): 301 - 306.
IEEE	ÇANKAYA M,ŞİŞECİOĞLU M,YÖRÜK Ö,ÖZDEMİR H "In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme." Turkish Journal of Medical Sciences, 36, ss.301 - 306, 2006.
ISNAD	ÇANKAYA, MURAT vd. "In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme". Turkish Journal of Medical Sciences 36/5 (2006), 301-306.