Isolation of DNA for sequence analysis from herbarium material of some lichen specimens

Emine Sümer ARAS, (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)
Demet CANSARAN (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)
Turkish Journal of Botany
4 0
Yıl: 2006 Cilt: 30 Sayı: 6 Sayfa Aralığı: 449 - 453 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation of DNA for sequence analysis from herbarium material of some lichen specimens

Öz:
Bazı likenlerin herbaryum örnekleri için geliştirilmiş bir DNA izolasyon yöntemi belirlenmiştir. Bu yöntemle izole edilmiş DNA, DNA dizi analizinin PCR reaksiyonları için uygundur. Bu çalışmada tanımlanmış olan hekzadesiltrimetilamonyum bromür (CTAB)'e dayalı bu protokol başta hız ve güvenilirlik olmak üzere birçok avantaj sağlamaktadır. Buna ek olarak liken türlerinin talluslarından elde edilen DNA'yı belirlemek için farklı DNA ekstraksiyon yöntemleri kullanılmıştır. Denenen metodlar hekzadesiltrimetilamonyum bromür (CTAB)'e ve sodyum dodesil sülfat (SDS)'a bağlı birer yöntemi kapsamaktadır. Bu prosedürler PCR analizi için uygun saflıkta DNA sağlamalarına karşın, geliştirilen protokol daha yüksek kalitede DNA sağlamaktadır. Bu protokol ile herbaryum materyalinden ortalama 25 mg/g DNA elde edilmesi mümkün olmuştur.
Anahtar Kelime: izolasyon teknikleri likenler herbaryumlar DNA DNA dizi analizi tallus izolasyon bitkiler

Konular: Biyoloji

Bazı likenlere ait herbaryum örneklerinde dizi analizi için DNA izolasyonu

Öz:
An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrimethylammonium bromide (CTAB) based protocol defined in this study provides a number of advantages, mainly speed and reliability. In addition, different DNA extraction protocols were examined to determine the yield of DNA from the thailus of lichen specimens. The methods examined include a CTAB based protocol and a sodium dodecyl sulphate (SDS) based protocol. Although these procedures yielded DNA of suitable purity for PCR analysis, the improved protocol yielded DNA of higher quality. The recovery of DNA with an average yield of 25 mg/g of herbarium material was possible with this procedure.
Anahtar Kelime: thallus isolation plants isolation techniques lichens herbaria DNA DNA sequencing

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Aras S, CANSARAN D (2006). Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. , 449 - 453.
Chicago Aras Sumer,CANSARAN Demet Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. (2006): 449 - 453.
MLA Aras Sumer,CANSARAN Demet Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. , 2006, ss.449 - 453.
AMA Aras S,CANSARAN D Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. . 2006; 449 - 453.
Vancouver Aras S,CANSARAN D Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. . 2006; 449 - 453.
IEEE Aras S,CANSARAN D "Isolation of DNA for sequence analysis from herbarium material of some lichen specimens." , ss.449 - 453, 2006.
ISNAD Aras, Sumer - CANSARAN, Demet. "Isolation of DNA for sequence analysis from herbarium material of some lichen specimens". (2006), 449-453.
APA Aras S, CANSARAN D (2006). Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. Turkish Journal of Botany, 30(6), 449 - 453.
Chicago Aras Sumer,CANSARAN Demet Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. Turkish Journal of Botany 30, no.6 (2006): 449 - 453.
MLA Aras Sumer,CANSARAN Demet Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. Turkish Journal of Botany, vol.30, no.6, 2006, ss.449 - 453.
AMA Aras S,CANSARAN D Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. Turkish Journal of Botany. 2006; 30(6): 449 - 453.
Vancouver Aras S,CANSARAN D Isolation of DNA for sequence analysis from herbarium material of some lichen specimens. Turkish Journal of Botany. 2006; 30(6): 449 - 453.
IEEE Aras S,CANSARAN D "Isolation of DNA for sequence analysis from herbarium material of some lichen specimens." Turkish Journal of Botany, 30, ss.449 - 453, 2006.
ISNAD Aras, Sumer - CANSARAN, Demet. "Isolation of DNA for sequence analysis from herbarium material of some lichen specimens". Turkish Journal of Botany 30/6 (2006), 449-453.
Sayfa Oluşturulma Tarihi
22-04-2024 12:34

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