Morphological studies on ovarian mast cells in the cow

ŞİMŞEK, Nejdet; Ergün, Emel; ERGUN, LEVENT; ÖZEN, Asuman

Morphological studies on ovarian mast cells in the cow

Asuman ÖZEN, (Ankara Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Levent ERGÜN, (Ankara Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Emel ERGÜN, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Histoloji Embriyoloji Bilim Dalı, Kırıkkale, Türkiye)

Nejdet ŞİMŞEK (Atatürk Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2007 Cilt: 31 Sayı: 2 Sayfa Aralığı: 131 - 136 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Morphological studies on ovarian mast cells in the cow

Öz:

Özet: Bu araştırma, östrual ve luteal fazlarda inek ovaryumunun farklı bölgelerindeki mast hücrelerinin boyanma özelliklerini, ışık ve elektron mikroskobik görünümlerini ve sayısal dağılımlarını belirlemek amacıyla yapıldı. Çalışmada 7'şer adet östrual ve luteal fazlardaki inek ovaryumu materyal olarak kullanıldı. Ovaryumda korpus luteum çevresinden, graff follikül çevresinden ve medulladan olmak üzere üç bölgeden parça alındı. Her üç bölgeden alınan parafin kesitlerinde toluidin blue ile metakromazi gösteren, alcian blue / safranin O (Ab/SO) kombine boyamasında Ab(+) / SO(-) boyanma özelliği gösteren mast hücrelerine rastlandı. Elektron mikroskobik incelemelerde mast hücrelerinin homojen yoğun ve ince tanecikli olmak üzere iki tip granül içerdiği gözlendi. Ovaryumun her üç bölgesinde de östrual fazda, luteal faza göre mm2>deki ortalama mast hücresi sayısının arttığı görüldü (P < 0,001). Estrual dönemde graaff follikülü çevresinde mast hücresi sayısında belirgin artış olurken, corpus luteum çevresinde az sayıda mast hücresine rastlandı. Ovaryumun üç bölgesi karşılaştırıldığında her iki fazda da mast hücre yoğunluğunun medullada olduğu gözlendi.

Anahtar Kelime: sarı cisim sığır elektron mikroskopisi kızgınlık dönemi kızgınlık yumurtalıklar inek yumurtalık follikülleri mast hücreleri Grafiyan folikülleri morfoloji

Konular: Ziraat Mühendisliği

İnekte ovarial mast hücreleri üzerine morfolojik çalışmalar

Öz:

Abstract: This study was conducted on cows in the estrual and luteal phases of the cycle to determine the staining properties, light and electron microscopic appearance and numerical distribution of mast cells from various areas of the ovary. The material for the study comprised ovarian specimen taken from 14 cows, 7 in the luteal phase and 7 in the estrual phase of the sexual cycle. Specimens were taken from 3 different areas of the ovaries, including those surrounding the corpus luteum, the graafian follicle and the medulla. From all 3 areas, mast cells were demonstrated as metachromatic staining after treatment of paraffin-embedded sections with toluidine blue and as Ab(+)/SO(-) by the combined alcian blue/safranine O (Ab/SO) stain. In the electron microscopic studies, the mast cells were observed to have 2 types of granules, namely homogeneously dense granules and tiny particulate granules. All 3 regions of the ovary were observed to have higher average mast cell counts per mm2 during the estrual phase than during the luteal phase (P < 0.001). In the estrual phase, while mast cell counts demonstrated a marked increase in the periphery of the graafian follicle, a low number of mast cells was determined in the periphery of the corpus luteum. In comparison, the medulla was demonstrated to have a much higher concentration of mast cells in the medulla than the other 2 regions of the ovarium.

Anahtar Kelime: ovarian follicles mast cells Graafian follicles morphology corpus luteum cattle electron microscopy oestrous cycle oestrus ovaries cows

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZEN A, ERGUN L, Ergün E, ŞİMŞEK N (2007). Morphological studies on ovarian mast cells in the cow. , 131 - 136.
Chicago	ÖZEN Asuman,ERGUN LEVENT,Ergün Emel,ŞİMŞEK Nejdet Morphological studies on ovarian mast cells in the cow. (2007): 131 - 136.
MLA	ÖZEN Asuman,ERGUN LEVENT,Ergün Emel,ŞİMŞEK Nejdet Morphological studies on ovarian mast cells in the cow. , 2007, ss.131 - 136.
AMA	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N Morphological studies on ovarian mast cells in the cow. . 2007; 131 - 136.
Vancouver	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N Morphological studies on ovarian mast cells in the cow. . 2007; 131 - 136.
IEEE	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N "Morphological studies on ovarian mast cells in the cow." , ss.131 - 136, 2007.
ISNAD	ÖZEN, Asuman vd. "Morphological studies on ovarian mast cells in the cow". (2007), 131-136.

APA	ÖZEN A, ERGUN L, Ergün E, ŞİMŞEK N (2007). Morphological studies on ovarian mast cells in the cow. Turkish Journal of Veterinary and Animal Sciences, 31(2), 131 - 136.
Chicago	ÖZEN Asuman,ERGUN LEVENT,Ergün Emel,ŞİMŞEK Nejdet Morphological studies on ovarian mast cells in the cow. Turkish Journal of Veterinary and Animal Sciences 31, no.2 (2007): 131 - 136.
MLA	ÖZEN Asuman,ERGUN LEVENT,Ergün Emel,ŞİMŞEK Nejdet Morphological studies on ovarian mast cells in the cow. Turkish Journal of Veterinary and Animal Sciences, vol.31, no.2, 2007, ss.131 - 136.
AMA	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N Morphological studies on ovarian mast cells in the cow. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(2): 131 - 136.
Vancouver	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N Morphological studies on ovarian mast cells in the cow. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(2): 131 - 136.
IEEE	ÖZEN A,ERGUN L,Ergün E,ŞİMŞEK N "Morphological studies on ovarian mast cells in the cow." Turkish Journal of Veterinary and Animal Sciences, 31, ss.131 - 136, 2007.
ISNAD	ÖZEN, Asuman vd. "Morphological studies on ovarian mast cells in the cow". Turkish Journal of Veterinary and Animal Sciences 31/2 (2007), 131-136.