The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids

YILDIZ, Levent; KAYA, Ekrem; CEYLAN, ALPER; KARA, Nurettin; GÜVEN, Hakan

The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids

Ekrem KAYA, (Uludağ Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Bursa, Türkiye)

Alper CEYLAN, (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Samsun, Türkiye)

Nurettin KARA, (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Moleküler Biyoloji Anabilim Dalı, Samsun, Türkiye)

Hakan GÜVEN, (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Samsun, Türkiye)

Levent YILDIZ (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Samsun, Türkiye)

Turkish Journal of Gastroenterology

3 0

Yıl: 2007 Cilt: 18 Sayı: 2 Sayfa Aralığı: 89 - 94 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids

Öz:

Amaç: ‹nşamatuvar barsak hastalığı gibi hastalıklı kolon mukozası na kısa zincirli yağ asidi ve glutaminin etkileri tam olarak aydınlatılamamıştır. Bu çalışmanın amacı deneysel kolitte L-glutamin ve kısa zincirli yağ asidi kokteyli lavmanı ve oral uygulamalarının kolon mukozası iyileşmesine etkilerini incelemektir. Gereç ve yöntem: Çalışmada sıçanlarda etanol içinde trinitrobenzensülfonik asid lavmanı ile kolit oluşturuldu. Gruplar; Serum fizyolojik lavmanı (Kolit grubu, n: 12), L-glutamin lavmanı grubu (n: 12), kısa zincirli yağ asidi lavmanı grubu (n: 12), oral l-glutamin grubu (n: 11) ve oral kısa zincirli yağ asidi grubu (n: 11) şeklinde idi. Lavman solusyonu kolit oluşturulduktan sonra on gün süre ile günde iki kere uygulandı. Sham grubunda kolit oluşturulmadan sadece serum fizyolojik lavmanı uygulandı (n: 12). Onuncu gün sakrifiye edilen sı- çanlarda kolon mukozasındaki kript derinliği ve mukozal DNA içeriği ölçüldü. Bulgular: Kript derinliği her iki glutamin grubu ve kısa zincirli yağ asidi lavmanı gruplarında sham ve kolit gruplarından fazla bulundu (p < 0.05). Mukozal DNA içeriği kolit ve her iki glutamin gruplarında kısa zincirli yağ asidi gruplarına göre daha fazla idi (p < 0.05). Oral glutamin grubu mukozal DNA içeriği de kısa zincirli yağ asidi lavmanı grubundan daha fazla idi (p < 0.05). Sonuç: Sıçanlarda deneysel kolitte L-glutamin mukozal iyileşme ve regenerasyonunu hızlandı rabilmektedir. Kısa zincirli yağ asidi kokteyli ise bu çalışmada glutamin kadar etkili bulunmamıştır.

Anahtar Kelime: İlaç uygulama, oral Hastalık modelleri, hayvan Glutamin Yenilenme DNA Kolit Asitler, asiklik Bağırsak mukozası Yara iyileşmesi Sıçan, Wistar

Konular: Cerrahi

Deneysel kolitte L-glutamin’in mukozal iyileşmeye etkisi kısa-zincirli yağ asitlerinden üstündür

Öz:

Background/aims: The effects of short-chain fatty acids and glutamine on diseased colonic mucosa, such as in inflammatory bowel disease, are not well described. The aim of this study was to investigate the role of L-glutamine and short-chain fatty acids, both via enema and oral administration, on mucosal healing in experimental colitis. Methods: Colitis was induced with trinitrobenzenesulphonic acid in ethanol enema in rats. Saline enema (Colitis group, n: 12), L-glutamine enema (n: 12), short-chain fatty acids enema (n: 12), oral L-glutamine (n:11) and oral short-chain fatty acids (n.11) were applied twice daily for 10 days after induction of colitis. The sham group (n: 12) received only saline enema. Rats were sacrificed at the tenth day. Crypt depth and DNA content were measured in colonic mucosa. Results: Crypt depth was significantly greater in both glutamine groups and short-chain fatty acids enema group than in sham and colitis groups (p<0.05). The mucosal DNA contents of the colitis and glutamine enema groups were significantly greater than both short-chain fatty acids groups (p<0.05). DNA content in the oral glutamine group was significantly greater than in the short-chain fatty acids enema group (p<0.05). Conclusions: L-glutamine enema can accelerate mucosal healing and regeneration in experimentally induced colitis in rats. When compared to glutamine in this study, shortchain fatty acids showed no beneficial effect on colitis.

Anahtar Kelime: Colitis Acids, Acyclic Intestinal Mucosa Wound Healing Rats, Wistar Administration, Oral Disease Models, Animal Glutamine Regeneration DNA

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KAYA E, CEYLAN A, KARA N, GÜVEN H, YILDIZ L (2007). The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. , 89 - 94.
Chicago	KAYA Ekrem,CEYLAN ALPER,KARA Nurettin,GÜVEN Hakan,YILDIZ Levent The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. (2007): 89 - 94.
MLA	KAYA Ekrem,CEYLAN ALPER,KARA Nurettin,GÜVEN Hakan,YILDIZ Levent The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. , 2007, ss.89 - 94.
AMA	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. . 2007; 89 - 94.
Vancouver	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. . 2007; 89 - 94.
IEEE	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L "The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids." , ss.89 - 94, 2007.
ISNAD	KAYA, Ekrem vd. "The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids". (2007), 89-94.

APA	KAYA E, CEYLAN A, KARA N, GÜVEN H, YILDIZ L (2007). The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turkish Journal of Gastroenterology, 18(2), 89 - 94.
Chicago	KAYA Ekrem,CEYLAN ALPER,KARA Nurettin,GÜVEN Hakan,YILDIZ Levent The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turkish Journal of Gastroenterology 18, no.2 (2007): 89 - 94.
MLA	KAYA Ekrem,CEYLAN ALPER,KARA Nurettin,GÜVEN Hakan,YILDIZ Levent The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turkish Journal of Gastroenterology, vol.18, no.2, 2007, ss.89 - 94.
AMA	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turkish Journal of Gastroenterology. 2007; 18(2): 89 - 94.
Vancouver	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids. Turkish Journal of Gastroenterology. 2007; 18(2): 89 - 94.
IEEE	KAYA E,CEYLAN A,KARA N,GÜVEN H,YILDIZ L "The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids." Turkish Journal of Gastroenterology, 18, ss.89 - 94, 2007.
ISNAD	KAYA, Ekrem vd. "The effect of L-glutamine on mucosal healing in experimental colitis is superior to short-chain fatty acids". Turkish Journal of Gastroenterology 18/2 (2007), 89-94.