Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey

GÖKCE, HALİL İBRAHİM; GÖKÇE, Gürbüz; GENÇ, Oktay; Sozmen, Mahmut

Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey

HALİL İBRAHİM GÖKCE, (Kafkas Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Kars, Türkiye)

Gürbüz GÖKÇE, (Kafkas Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Kars, Türkiye)

Oktay GENÇ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Kontrol Bölümü, Samsun,Türkiye)

Mahmut SÖZMEN (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

3 0

Yıl: 2007 Cilt: 31 Sayı: 5 Sayfa Aralığı: 355 - 360 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey

Öz:

Bu çalışmada Kars ilinde aniden ölen ve enterotoksemi şüphesi bulunan koyunlarda bu hastalığa neden olan Clostridium perfringens tiplerini ve bu bakterinin üretmiş olduğu toksinlerin belirlenmesi amaçlandı. Çalışmada Kars ilinde enterotoksemi şüphesi ile ölmüş olan çeşitli ırk ve yaştaki 260 adet ve mezbahada kesilen 35 adet sağlıklı koyun kullanıldı. Bu amaçla her bir koyunun ince bağırsak içeriği toplandı ve bu içerikde enzyme-linked immunosorbent assay (ELISA) ve lateks agglutinasyon testi (LAT) ile C. perfringens tipleri tarafından üretilen alfa, beta ve epsilon toksinlerin varlığı araştırıldı. Çalışmada, toplanan 260 bağırsak içeriğinden ELISA yöntemiyle ile 220’sinde (% 84,61) ve LAT ile ise 152’sinde (% 58,46) C. perfringens toksinlerine rastlandı. Bu örneklerin 40’ı (% 15,38) ELISA ve 108’i (% 41,53) LAT ile negatif olarak belirlendi. Yapılan biyo-tiplendirmede ELISA yöntemi ile pozitif olarak belirlenen örneklerin sırasıyla 104’ü (% 47,27), 20’si (% 9,09), 10’u (% 4,54) ve 86’sı (% 39,07) C. perfringens tip A, B, C ve D olarak idenfiye edildi. LAT testi ile bakterinin yapılan biyo-tiplendirilmesinde ise örneklerin 79’u (% 51,97) tip A, 15’i (% 9,86) tip B, 7’si (% 4,60) tip C ve 51’i (% 33,55) tip D olarak idenfiye edildi. Her iki testte de en yüksek pozitişik C. perfringens tip A ve tip D için bulundu. Yapılan bu çalışmadan elde edilen sonuçlara göre ve Kars ilinde 300.000 civarında koyunun bulunması nedeniyle bu ilde enterotoksemiden kaynaklanan ekonomik kaybın epeyce büyük olduğu ortaya çıkmaktadır. Bu nedenle de Kars ilinde bulunan koyunların enterotoksemiye karşı düzenli bir şekilde aşılanması ve aşıların mutlaka C. perfringens tip A ve D’ye karşı bağışıklık sağlayacak şekilde düzenlenmesi önerilmektedir.

Anahtar Kelime: enterotoksemi enterotoksin lateks aglütinasyon testi Clostridium perfringens bağışıklık ELISA

Konular: Ziraat Mühendisliği

Kars ilinde enterotoksemi şüphesi ile ölen koyunlarda Clostridium perfringens’in toksinlerinin belirlenmesi

Öz:

The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Kars province. For this purpose, 260 dead sheep with suspected enterotoxemia and 35 clinically healthy sheep (controls) of different ages and breeds were used. Intestinal contents of each sheep were collected and used to determine alpha, beta, and epsilon toxins of C. perfringens using enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT). Based on ELISA and LAT, 220 (84.61%) and 152 (58.46%) sheep, respectively, were positive for C. perfringens toxins. In contrast, 40 (15.38%) sheep were toxin-negative based on ELISA and 108 (41.53%) were toxin-negative based on LAT. With ELISA, 104 (47.27%), 20 (9.09%), 10 (4.54%), and 86 (39.07%) toxin-positive samples were identified as C. perfringens type A, B, C, and D, respectively, whereas with LAT, 79 (51.97%), 15 (9.86%), 7 (4.60%), and 51 (33.55%) toxin-positive samples were identified as type A, B, C, and D, respectively. C. perfringens type A and type D were the dominant types detected by both tests in sheep with suspected enterotoxemia, in Kars province. It is concluded that enterotoxemia causes considerable economic loss to the sheep industry in Kars province, where 300,000 sheep are present. Therefore, it is recommended that a proper vaccination schedule against enterotoxemia be implemented for flocks in Kars province. These vaccines should provide adequate protective immunity against both C. perfringens type A and type D. Key Words: Sheep, Clostridium perfringens, alpha toxin, beta toxin, epsilon toxin, enterotoxaemia, enzyme-linked immunosorbent assay (ELISA), latex agglutination test (LAT)

Anahtar Kelime: enterotoxins latex agglutination test Clostridium perfringens immunity ELISA enterotoxaemia

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÖKCE H, GÖKÇE G, GENÇ O, Sozmen M (2007). Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. , 355 - 360.
Chicago	GÖKCE HALİL İBRAHİM,GÖKÇE Gürbüz,GENÇ Oktay,Sozmen Mahmut Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. (2007): 355 - 360.
MLA	GÖKCE HALİL İBRAHİM,GÖKÇE Gürbüz,GENÇ Oktay,Sozmen Mahmut Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. , 2007, ss.355 - 360.
AMA	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. . 2007; 355 - 360.
Vancouver	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. . 2007; 355 - 360.
IEEE	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M "Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey." , ss.355 - 360, 2007.
ISNAD	GÖKCE, HALİL İBRAHİM vd. "Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey". (2007), 355-360.

APA	GÖKCE H, GÖKÇE G, GENÇ O, Sozmen M (2007). Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. Turkish Journal of Veterinary and Animal Sciences, 31(5), 355 - 360.
Chicago	GÖKCE HALİL İBRAHİM,GÖKÇE Gürbüz,GENÇ Oktay,Sozmen Mahmut Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. Turkish Journal of Veterinary and Animal Sciences 31, no.5 (2007): 355 - 360.
MLA	GÖKCE HALİL İBRAHİM,GÖKÇE Gürbüz,GENÇ Oktay,Sozmen Mahmut Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.31, no.5, 2007, ss.355 - 360.
AMA	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(5): 355 - 360.
Vancouver	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(5): 355 - 360.
IEEE	GÖKCE H,GÖKÇE G,GENÇ O,Sozmen M "Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey." Turkish Journal of Veterinary and Animal Sciences, 31, ss.355 - 360, 2007.
ISNAD	GÖKCE, HALİL İBRAHİM vd. "Determination of Clostridium perfringens toxin-types in sheep with suspected Enterotoxemia in Kars province, Turkey". Turkish Journal of Veterinary and Animal Sciences 31/5 (2007), 355-360.