Virulence properties of Escherichia coli isolated from clinical bovine mastitis

GÜLER, Leyla; GÜNDÜZ, Kadri

Virulence properties of Escherichia coli isolated from clinical bovine mastitis

Leyla GÜLER, (Veteriner Kontrol ve Araştırma Enstitüsü Müdürlüğü, Konya, Türkiye)

Kadri GÜNDÜZ (Veteriner Kontrol ve Araştırma Enstitüsü Müdürlüğü, Konya, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2007 Cilt: 31 Sayı: 5 Sayfa Aralığı: 361 - 365 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Virulence properties of Escherichia coli isolated from clinical bovine mastitis

Öz:

Bu çalışmada ineklerin klinik mastitis vakalarından izole edilen E. coli suşlarında bazı virülens faktör genlerinin araştırılması amaçlandı. Her biri farklı sürülere ait, 2000-2005 yılları arasında izole edilen toplam 100 E. coli suşu incelendi. Shiga toxin 1 ve 2 (stx1 ve stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), F5 (K99), F41 ve F17 fimbria’larını kodlayan genlerin tespiti amacıyla iki ayrı multipleks PCR uygulandı. PCR testleri sonucu 9 suş F17 fimbria geni ve sadece 1 suş eaeA geni pozitif bulundu. Sonuçlar klinik inek mastitislerinden izole edilen E. coli suşlarında araştırılan potansiyel virülens faktörlerinin hiç birinin yaygın olarak bulunmadığını gösterdi. İneklerde mastitis vakalarından izole edilen E. coli suşları arasında PCR yöntemi ile insanlarda hastalık oluşturma ihtimaline sahip Shiga toksin üreten E. coli (STEC) tespit edilmedi.

Anahtar Kelime: sığır virülans meme yangısı enterotoksin bakteriyel toksinler Escherichia coli sığır meme yangısı genler

Konular: Ziraat Mühendisliği

Klinik inek mastitislerinden izole edilen Escherichia coli suşlarının virülens özellikleri

Öz:

The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis. A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined. Two multiplex polymerase chain reaction (PCR) assays were used to detect the presence of the genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), and F5 (K99), F41, and F17 fimbriae. In the PCR assays, 9 isolates were positive for the genes of F17 fimbriae and only 1 isolate was positive for the eaeA gene. The results demonstrated that none of the potential virulence factors investigated was commonly observed in E. coli isolates from bovine clinical mastitis. Shiga toxin-producing E. coli (STEC), which is potentially pathogenic for humans, was not detected by PCR among the isolates from the cases of bovine mastitis.

Anahtar Kelime: enterotoxins bacterial toxins Escherichia coli bovine mastitis genes cattle virulence mastitis

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÜLER L, GÜNDÜZ K (2007). Virulence properties of Escherichia coli isolated from clinical bovine mastitis. , 361 - 365.
Chicago	GÜLER Leyla,GÜNDÜZ Kadri Virulence properties of Escherichia coli isolated from clinical bovine mastitis. (2007): 361 - 365.
MLA	GÜLER Leyla,GÜNDÜZ Kadri Virulence properties of Escherichia coli isolated from clinical bovine mastitis. , 2007, ss.361 - 365.
AMA	GÜLER L,GÜNDÜZ K Virulence properties of Escherichia coli isolated from clinical bovine mastitis. . 2007; 361 - 365.
Vancouver	GÜLER L,GÜNDÜZ K Virulence properties of Escherichia coli isolated from clinical bovine mastitis. . 2007; 361 - 365.
IEEE	GÜLER L,GÜNDÜZ K "Virulence properties of Escherichia coli isolated from clinical bovine mastitis." , ss.361 - 365, 2007.
ISNAD	GÜLER, Leyla - GÜNDÜZ, Kadri. "Virulence properties of Escherichia coli isolated from clinical bovine mastitis". (2007), 361-365.

APA	GÜLER L, GÜNDÜZ K (2007). Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turkish Journal of Veterinary and Animal Sciences, 31(5), 361 - 365.
Chicago	GÜLER Leyla,GÜNDÜZ Kadri Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turkish Journal of Veterinary and Animal Sciences 31, no.5 (2007): 361 - 365.
MLA	GÜLER Leyla,GÜNDÜZ Kadri Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turkish Journal of Veterinary and Animal Sciences, vol.31, no.5, 2007, ss.361 - 365.
AMA	GÜLER L,GÜNDÜZ K Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(5): 361 - 365.
Vancouver	GÜLER L,GÜNDÜZ K Virulence properties of Escherichia coli isolated from clinical bovine mastitis. Turkish Journal of Veterinary and Animal Sciences. 2007; 31(5): 361 - 365.
IEEE	GÜLER L,GÜNDÜZ K "Virulence properties of Escherichia coli isolated from clinical bovine mastitis." Turkish Journal of Veterinary and Animal Sciences, 31, ss.361 - 365, 2007.
ISNAD	GÜLER, Leyla - GÜNDÜZ, Kadri. "Virulence properties of Escherichia coli isolated from clinical bovine mastitis". Turkish Journal of Veterinary and Animal Sciences 31/5 (2007), 361-365.