İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi
Yıl: 2007 Cilt: 18 Sayı: 3 Sayfa Aralığı: 117 - 120 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi
Öz: bileşik olarak, üzüm, fındık, kırmızı şarap ve çeşitli yiyeceklerde çok bulunmaktadır. Resveratrol, antiinflamatör, antioksidan ve antikanser özellikleri vardır. Bu çalışmada İnsan Epidermoid Larinks HEp-2 hücre serisinde, resveratrolün apoptotik etkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: İnsan Epidermoid Larinks kanseri HEp-2 hücre serileri üretildi ve sonra farklı doz ve sürelerde resveratrolün etkisine maruz bırakıldı. Daha sonra apoptotik hücre ölümleri, yapısal değişiklikler DNA fragmantasyon metotları ile belirlendi. Bulgular: Resveratrolün doz ve süreye bağımlı olarak HEp-2 hücre dizilerinde hücre ölümünü uyardığı, morfolojik ve internükleosomal DNA fragmantasyon deneyleri ile gösterildi. Sonuç: Resveratrolün HEp-2 hücre dizilerinde apoptotik etkisi, belirli kanser türlerindeki tedavilerde yardımcı olabileceğini göstermektedir.
Anahtar Kelime: Konular:
The apoptotic effect of resveratrol on human epidermoid larynx carcinoma cell line HEp-2
Öz: EPIDERMOID LARYNX CARCINOMA CELL LINE HEp-2 Purpose: Resveratrol (trans-3,4’,5-trihydroxystilbene) is a naturally occurring polyphenolic compound highly enriched in grapes, nuts, red wine, and a wide variety of food sources. Resveratrol has anti-inflammatory, antioxidant, and anticancer properties. In this study, Human Epidermoid Larynx HEp-2 cell lines were used to examine the apoptotic effect of resveratrol. Materials and Methods: Human Epidermoid Larynx HEp-2 cell lines were cultured and then exposed to different concentrations of resveratrol for various periods. After treatments, apoptotic cell deaths were determined using morphological changes and DNA fragmentation methods. Results: Our data show that resveratrol induces dose- and time-dependent death of HEp-2 cells, as measured morphologically and by internucleosomal DNA fragmentation assay. Conclusion: Our data suggest that the apoptotic effect of resveratrol on HEp-2 cell lines may be useful for the treatment of certain types of cancer.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1- Gusman J, Malonne H, Atassi G. A reappraisal of the potential chemopreventive and chemotherapeutic properties of resveratrol. Carcinogenesis 2001; 22: 1111-1117.
- 2- Dong Z. Molecular mechanism of the chemopreventive effect of resveratrol. Mutat Res 2003; 523-524: 145-150.
- 3- Latruffe N, Delmas D, Jannin D, Malki MC, Passilly-Degrace P, Berlot JP. Molecular analysis on the chemopreventive proporties of resveratrol, a plant polyphenol microcomponent. Int J Mol Med 2002; 10: 755-760.
- 4- Park JW, Choi YJ, Suh SI, Baek WK, Suh MH, Jin IN, Min DS, Woo JH, Chang JS, Passaniti A, Lee YH, Kwon TK. Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity. Carcinogenesis 2001; 22: 1633-1639.
- 5- Bohm I, Schild H. The complex scenario for a silent cell death. Mol Imaging Biol 2003; 5: 2-14.
- 6- Saraste A, Pulkki K. Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 2000; 45: 528-537.
- 7- Niles RM, McFarland M, Weimer MB, Redkar A, Fu YM, Meadows GG. Resveratrol is a potent inducer of apoptosis in human melanoma cells. Cancer Lett 2003; 190: 157-163.
- 8- Roy M, Chakraborty S, Siddiqi M, Bhattacharya RK. Induction of apoptosis in tumor cells by natural phenolic compounds. Asian Pac J Cancer Prev 2002; 3: 61-67.
- 9- Yurtcu E, Ergun MA, Menevse A. Apoptotic effect of gossypol on human lymphocytes. Cell Bio Int 2003; 27: 791-794.
- 10- Jiang H, Zhang L, Kuo J, Kuo K, Gautam SC, Groc L, Rodriguez AI, Koubi D, Hunter TJ, Corcoran GB, Seidman MD, Levine RA. Resveratrol-induced apoptotic death in human U251 glioma cells. Mol Cancer Ther. 2005; 4: 554-561.
- 11- Hsieh TC, Halicka D, Lu X, Kunicki J, Guo J, Darzynkiewicz Z, Wu JM. Effects of resveratrol on the G0-G1 transition and cell cycle progression of mitogenically stimulated human lymphocytes. Biochem Biophys Res Commun 2002; 297: 1311-1317.
- 12- Joe AK, Liu H, Suzui M, Vural EM, Xiao D, Weinstein IB. Resveratrol induces growth inhibition, S-phase arrest, apoptosis and changes in biomarker expression in several human cancer cell lines. Clin Cancer Res 2002; 8: 893-903.
- 13- Pozo-Guisado E, Alvarez-Barrientos A, Mulero-Navarro S, Santiago- Josefat B, Fernandez-Salguero PM. The antiproliferative activity of resveratrol results in apoptosis in MCF-7 but not in MDA-MB-231 human breast cancer cells: cell-specific alteration of the cell cycle. Biochem Pharmacol 2002; 64: 1375-1386.
- 14- Ferry-Dumazet H, Garnier O, Mamani-Matsuda M, Vercauteren J, Belloc F, Billiard C, Dupouy M, Thiolat D, Kolb JP, Marit G, Reiffers J, Mossalayi MD. Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells. Carcinogenesis 2002; 23: 1327-1333.
- 15- Clément MV, Hirpara JH, Chawdhury SH, Pervaiz S. Chemopreventive agent resveratrol, a natural product derived from grapes, triggers CD95 signalling-dependent apoptosis in human tumor cells. Blood 1998; 92: 996-1002.
| APA | TÜRKMEN D, YURTCU E, ERGÜN M, MENEVŞE A (2007). İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. , 117 - 120. |
| Chicago | TÜRKMEN Dilek,YURTCU Erkan,ERGÜN M. Ali,MENEVŞE Adnan İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. (2007): 117 - 120. |
| MLA | TÜRKMEN Dilek,YURTCU Erkan,ERGÜN M. Ali,MENEVŞE Adnan İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. , 2007, ss.117 - 120. |
| AMA | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. . 2007; 117 - 120. |
| Vancouver | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. . 2007; 117 - 120. |
| IEEE | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A "İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi." , ss.117 - 120, 2007. |
| ISNAD | TÜRKMEN, Dilek vd. "İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi". (2007), 117-120. |
| APA | TÜRKMEN D, YURTCU E, ERGÜN M, MENEVŞE A (2007). İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. Gazi Medical Journal, 18(3), 117 - 120. |
| Chicago | TÜRKMEN Dilek,YURTCU Erkan,ERGÜN M. Ali,MENEVŞE Adnan İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. Gazi Medical Journal 18, no.3 (2007): 117 - 120. |
| MLA | TÜRKMEN Dilek,YURTCU Erkan,ERGÜN M. Ali,MENEVŞE Adnan İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. Gazi Medical Journal, vol.18, no.3, 2007, ss.117 - 120. |
| AMA | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. Gazi Medical Journal. 2007; 18(3): 117 - 120. |
| Vancouver | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi. Gazi Medical Journal. 2007; 18(3): 117 - 120. |
| IEEE | TÜRKMEN D,YURTCU E,ERGÜN M,MENEVŞE A "İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi." Gazi Medical Journal, 18, ss.117 - 120, 2007. |
| ISNAD | TÜRKMEN, Dilek vd. "İnsan epidermoid larinks karsinom (HEp-2) hücre serisinde resveratsol'ün apoptotik etkisi". Gazi Medical Journal 18/3 (2007), 117-120. |
