The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions

yıldız, mehmet; DELEN, Nafiz; COŞKUNTUNA, ARZU; YILDIZ, FIGEN; Türküsay, Hüseyin; KINAY TEKSÜR, PERVİN

The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions

Figen YILDIZ, (Ege Üniversitesi, Ziraat Fakültesi, İzmir, Türkiye)

Mehmet YILDIZ, (Ege Üniversitesi, Ziraat Fakültesi, İzmir, Türkiye)

Nafiz DELEN, (Ege Üniversitesi, Ziraat Fakültesi, İzmir, Türkiye)

Arzu COŞKUNTUNA, (Namık Kemal Üniversitesi, Ziraat Fakültesi, Tekirdağ, Türkiye)

PERVİN KINAY TEKSÜR, (Ege Üniversitesi, Ziraat Fakültesi, İzmir, Türkiye)

Hüseyin TÜRKÜSAY (Ege Üniversitesi, Ziraat Fakültesi, İzmir, Türkiye)

Turkish Journal of Agriculture and Forestry

2 0

Yıl: 2007 Cilt: 31 Sayı: 5 Sayfa Aralığı: 319 - 325 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions

Öz:

Sera bölgelerinden alınan domates yapraklarından izole edilen 163 bakterinin, kurşuni küf hastalığını (Botrytis cinerea) baskılayıcı etkisi bir seri testle ortaya konmuştur. Domates bitkilerinde kurşuni küf hastalığına karşı bu antagonist bakterilerinden özellikle dört adedi hastalığı baskılamada etkili olmuş ve bunlar Pseudomonas fluorescens olarak tanılanmıştır. Domates seralarında kullanılan fungisitlerin bu bakterileri etkilemedikleri testlerle saptanmıştır. Bunun sonucunda, 4 bakterinin sera koşullarında, fenhexamidin düşük dozuyla, hastalığa etkisi araştırılmıştır. Bu bakterilerden bir tanesi (Pf163) serada yapılan testlerde kurşuni küf hastalığını %78 oranında azaltmıştır. Kontrollerle karşılaştırıldığında, Pseudomonas fluorescens’in diğer izolatlarının fenhexamidin düşük dozu (12.5 ml a.i. hl-1) ile birlikte hastalığı sırasıyla %74.17, %70.52 ve %65.74 oranında baskıladığı ortaya konmuştur. Bakteriyel antagonistlerin serada yetiştirilen bitkilerdeki hastalığı engellemede ve fungisitlerle birlikte kullanımlarında potansiyel bir etkileri olduğu ortaya konmuştur.

Anahtar Kelime:

Konular: Orman Mühendisliği

Sera domates yetiştiriciliğinde görülen kurşuni küf hastalığı üzerinde kimyasal ve biyolojik uygulamaların etkileri

Öz:

A total of 163 bacterial strains isolated from tomato leaves were evaluated for their ability to suppress gray mold (Botrytis cinerea Pers. ex Fr.) on tomatoes, and 4 strains consistently reduced the incidence of B. cinerea. These antagonist strains were identified as Pseudomonas fluorescens. The bacterial strains with in vitro resistance to fungicides were combined with low-dose fenhexamid in vivo. One strain of P. fluorescens (Pf163) reduced the disease incidence by an average of 78% on tomato plants inoculated with B. cinerea in greenhouse trials. In comparison to the control treatment, other P. fluorescens strains applied with low-dose fenhexamid reduced the incidence of decay by 74.17%, 70.52%, and 65.74%, respectively. The antagonists significantly reduced gray mold decay when combined with low-dose (12.5 ml a.i. hl-1) fenhexamid more than the control did. It was shown that antagonistic bacterial strains inhibited B. cinerea and that they have a potential use in combination with the fungicide in greenhouse experiments.

Anahtar Kelime:

Konular: Orman Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Blakeman, J.P. and N.J. Fokkema. 1982. Potential for biological control of plant diseases on the phylloplane. Annu. Rev. Phytopathol. 20: 167-192.
Buchanan, R.E. and N.E. Gibbons. 1974. Bergey’s Manual of Determinative Bacteriology. Eight Edition. Waverly Press, Baltimore, USA.
Card, S., M. Jaspers, M. Walters and A. Stewart. 2002. Evaluation of microorganisms for biocontrol of grey mould on lettuce. N.Z. Plant Prot. 55: 197-201.
Delen, N., M. Yıldız and H. Maraite. 1984. Benzimidazole and Dithiocarbamate resistance of B. cinerea on greenhouse crops in Turkey. Med. Fac. Landbauw. Rijk. Gent. pp. 153-161.
Delen, N. and T. Özbek. 1992. Effectiveness of some fungicides and fungicide combinations on B. cinerea isolates. In: Recent Advances in Botrytis Research. (Eds.: K. Verhoeff, N.E. Malathrakis and B. Williamson). Pudoc Scientific Publisher, Wageningen, pp. 217-222.
Delen, N. and T. Özbek. 1994. Some major fungal and bacterial disease of solanaceous vegetables in greenhouses and characterization of their control methods in Turkiye. Acta Hort. 336: 307-315.
Delen, N. and N.Tosun. 1996. Reduced sensitivity in B. cinerea to Thiram and Mancozeb. In: Proceedings of XI International Botrytis Symposium, Program and Book of Abstracts, pp. 31.
Delen, N., N. Tosun, Ö. Yılmaz and Z. Yıldız. 2000. Variation in the sensitivities of B. cinerea isolates to some fungicides with nonspecific mode of action. In: Proceedings of XII International Botrytis Symposium. Reihms, France, pp. 64.
Dik, A., G. Goning and J. Kohl. 1999. Evaluation of microbial antagonists for biological control of B. cinerea stem infection in cucumber and tomato. Eur. J. Plant Pathol. 105: 115-122.
Dubos, B. 1992. Biological Control of Botrytis State-of-the-art, In: Recent Advances in Botrytis Research (Eds: K. Verhoeff,, N.E. Malathrakis and B. Williamson). Pudoc Scientific Publisher, Wageningen, pp.169-178.
Elad, Y., D, Shtienberg and A. Niv. 1994. Trichoderma harzianum T39 integrated with fungicides: Improved biocontrol of grey mould. Brighton Crop Protection Conference, Pests and Diseases. pp. 1109-1114.
Elad., Y., J. Köhl and N.J. Fokkema. 1994. Control of infection and sporulation of B. cinerea on bean and tomato by saprophytic bacteria and fungi. Eur. J. Plant Pathol. 100: 315-336.
Elad, Y. 2000. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Prot. 19: 709-714.
Elad, Y. and S. Freeman. 2002. Biological control of fungal pathogens. In: The Mycota, A comprehensive treats on fungi as experimental systems for basic and applied research. Vol. VI. Agricultural Applications (Ed.: F. Kempken), Springer, Heidelberg, Germany, pp. 93-109.
Gould, A.B., D.Y. Kobayashi and M.S. Bergen. 1996. Identification of bacteria for biological control of B. cinerea on petunia using a petal disk assay. Plant Dis. 80: 1029-1033.
Haenssler, G., A. Study and D. Stuebler. 2000. Uptake, sensitivity profile and biochemical mode of action of the new Botryticide fenhexamid. In: Proceedings of XII International Botrytis Symposium. Reihms, France, pp 33.
Jarvis, W.R. 1977. Botryotinia and Botrytis species: Taxonomy, Physiology and Pathology. Monogr. 15, Research Branch, Canada Department of Agriculture, Research Station, Harroe, Ottawa, Canada.
Malathrakis, N., E. Marlellou and B. Seddon. 1996. Integrated pest management of Botrytis. In: Proceedings of XI International Botrytis Symposium, Wageningen, The Netherlands, pp.95.
Maraite, H., G. Gilles, S.Meunier, J. Weyns and E.Bal. 1980. Resistance of B. cinerea Pers. to Dicarboximide fungicides in strawberry fields. Parasitica 36: 90-01.
Nicot, P., N. Morrison and V. Decognet. 1996. Protection of pruning wounds on greenhouse tomatoes by saprophytic microorganisms against infection by B. cinerea. In: Proceedings of XI International Botrytis Symposium, Wageningen, The Netherlands, pp. 70.
Redmond, J.C., J.J. Marois and J.D. Macdonald. 1987. Biological control of B.cinerea with epiphytic microorganisms. Plant Dis. 71:799-802.
Shtienberg, D., Y. Elad, Y. Nitzani and B. Kirshner. 1996. Botman-A decision support system for the management of Botrytis in greenhouse vegetables. In: Proceedings of XI International Botrytis Symposium. Wageningen, The Netherlands, pp. 96.
Shtienberg, D. and Y. Elad. 1997. Incorporation of weather forecasting in integrated, biological-chemical management of Botrytis cinerea. Phytopathology 87: 332-340.
Swadling, I.R. and P. Jeffries. 1998. Antagonistic properties of two bacterial biocontrol agents of grey mould disease. Biocontrol Sci. Techn. 8: 439-448.
Study, A., R. Ponzen and K. Stengel. 1999. Fenhexamid-sensitivity of B. cinerea: Determination of baseline sensitivity and assessment of risk of resistance. Phlanzenschutz Nachrichten, Bayer. 52: 146-157.
Utkhede, R., C. Bogdanoff and J. Mcnenin. 2001. Effects of biological and chemical treatments on Botrytis stem cancer and fruit yield of tomato under greenhouse conditions. Can. J. Plant Pathol. 23:253-259.
Yıldız, M. and N. Delen. 1985. Beobachtungen uber die fungizideanwendungen in gemüseangebauten gewaechshaeusern. In: Proceedings of IV Turkish Phytopathology Congress, Izmir.pp. 103.
Yıldız, F. 2000. Studies on the Biological Control of Grey Mould Disease (Botrytis cinerea Pers.) of the Greenhouse Grown Tomatoes. J. Turk. Phytopath. 29: 95-103.

APA	YILDIZ F, yıldız m, DELEN N, COŞKUNTUNA A, KINAY TEKSÜR P, Türküsay H (2007). The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. , 319 - 325.
Chicago	YILDIZ FIGEN,yıldız mehmet,DELEN Nafiz,COŞKUNTUNA ARZU,KINAY TEKSÜR PERVİN,Türküsay Hüseyin The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. (2007): 319 - 325.
MLA	YILDIZ FIGEN,yıldız mehmet,DELEN Nafiz,COŞKUNTUNA ARZU,KINAY TEKSÜR PERVİN,Türküsay Hüseyin The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. , 2007, ss.319 - 325.
AMA	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. . 2007; 319 - 325.
Vancouver	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. . 2007; 319 - 325.
IEEE	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H "The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions." , ss.319 - 325, 2007.
ISNAD	YILDIZ, FIGEN vd. "The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions". (2007), 319-325.

APA	YILDIZ F, yıldız m, DELEN N, COŞKUNTUNA A, KINAY TEKSÜR P, Türküsay H (2007). The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. Turkish Journal of Agriculture and Forestry, 31(5), 319 - 325.
Chicago	YILDIZ FIGEN,yıldız mehmet,DELEN Nafiz,COŞKUNTUNA ARZU,KINAY TEKSÜR PERVİN,Türküsay Hüseyin The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. Turkish Journal of Agriculture and Forestry 31, no.5 (2007): 319 - 325.
MLA	YILDIZ FIGEN,yıldız mehmet,DELEN Nafiz,COŞKUNTUNA ARZU,KINAY TEKSÜR PERVİN,Türküsay Hüseyin The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. Turkish Journal of Agriculture and Forestry, vol.31, no.5, 2007, ss.319 - 325.
AMA	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. Turkish Journal of Agriculture and Forestry. 2007; 31(5): 319 - 325.
Vancouver	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions. Turkish Journal of Agriculture and Forestry. 2007; 31(5): 319 - 325.
IEEE	YILDIZ F,yıldız m,DELEN N,COŞKUNTUNA A,KINAY TEKSÜR P,Türküsay H "The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions." Turkish Journal of Agriculture and Forestry, 31, ss.319 - 325, 2007.
ISNAD	YILDIZ, FIGEN vd. "The effects of biological and chemical treatment on gray mold disease in tomatoes grown under greehouse conditions". Turkish Journal of Agriculture and Forestry 31/5 (2007), 319-325.