Although standard tube agglutination (STA) test is the most preferredmethod for the serodiagnosis of brucellosis, it may yield false negative results becauseof its incompetence in detecting the blocking (non-agglutinating) antibodies. Blockingantibodies can be detected by Coombs’ test (anti-human globulin test), which is generally not performed in laboratories routinely since it is a time consuming andlaborious method. Recently more practical Brucellacapt (immunocapture-agglutination)test is being used for the detection of total antibodies without any interference of thepresence of blocking ones. The aim of this study was the evaluation of the value ofBrucellacapt test in the serodiagnosis of brucellosis. Twenty-five brusellosis patientswhose blood cultures yielded Brucella melitensis growth, and 31 healthy subjects ascontrols, were included to the study. Serum samples obtained from all of the 56 subjectshave been searched by STA, Coombs’ and Brucellacapt methods, and threshold titrewas accepted as 1/160. As a result, 40% (10/25) of culture positive patients werefound positive (≥1/160) by STA, whereas 92% (23/25) were positive (≥1/160) byboth Coombs’ and Brucellacapt tests. No positivity was detected in the control groupby any one of those three tests. The agreement between Coombs and Brucellacaptmethods was found as 100%, while the sensitivity and specificity of Brucellacapt testwere estimated as 92% (23/25) and 100% (31/31), respectively, when the culture wasaccepted as reference method. Although our data have pointed out the high performanceof Brucellacapt test in brusellosis serodiagnosis, confirmation with further data carriedon extensive and comprehensive study groups, are needed.