Relationship between activation and apoptosis in platelets

ÖZEROL, Elif; OZGEN, ÜNSAL; AMİNCİ, Mehmet

Relationship between activation and apoptosis in platelets

Ünsal ÖZGEN, (İnönü Üniversitesi, Tıp Fakültesi, Pediatrik Hematoloji Anabilim Dalı, Malatya, Türkiye)

Elif ÖZEROL, (İnönü Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Malatya, Türkiye)

Mehmet AMİNCİ (İnönü Üniversitesi, Tıp Fakültesi, Pediatri Anabilim Dalı, Malatya, Türkiye)

Turkish Journal of Hematology

1 0

Yıl: 2007 Cilt: 24 Sayı: 4 Sayfa Aralığı: 171 - 177 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Relationship between activation and apoptosis in platelets

Öz:

Çekirdekli hücrelerde fosfatidilserinin (PS) hücre zarı dış yüzeyine çıkması bir apoptoz belirteci olup trombositlerde apoptozdan ziyade aktivasyonu düşündürmektedir. Diğer taraftan, çekirdekli hücrelerdeki apoptoz ve trombositlerdeki aktivasyon arasında birçok benzerlik bulunmaktadır. Bu çalışmada trombosit aktivasyonu ve apoptozu arasındaki ilişkiyi araştırdık. Sağlıklı 22 vericiden taze olarak alınan kandan izole edilen trombositler calcium ionophore A23187 bulunan ve bulunmayan tüplerde inkube edildi. Trombosit aktivasyon belirteci olarak CD62P ve CD63, apoptozunun belirteci olarak anti-caspase-3 antikoru ve JC-1 katyonik boyası, hücre dış yüzeyindeki PS tespiti için ise Annexin-V kullanıldı. Tüm belirteçler A23187 ile inkubasyon başlangıcında, 20. dakikada ve 5. saatte akım sitometrisi ile çalışıldı. İnkübasyonun 5. saatinde caspase 3 aktivasyonu ile PS ekternalizasyonu, ∆Ψm depolarizasyonu, CD63 arasında ve ayrıca PS eksternalizasyonu ile CD62P arasında anlamlı korelasyon bulundu. Bu bulgular trombosit aktivasyonu ve apoptozu arasında bir ilişkinin olduğunu, trombosit aktivasyonunun apoptoza ilerlediğini düşündürmektedir.

Anahtar Kelime: Kan trombositleri Apoptoz Fosfatidilserinler Trombosit aktivasyonu Anneksin A5

Konular: Hematoloji

Trombosit aktivasyonu ve apoptozu arasındaki ilişki

Öz:

Phosphatidylserine (PS) externalization is a marker for the nucleated cell apoptosis, and refers cellular activation rather than apoptosis in platelets. On the other hand, several similarities exist between platelet activation and apoptosis in nucleated cells. Herewith we investigated the relationship between platelet activation and platelet apoptosis. Platelets isolated from fresh blood of 22 healthy volunteers were incubated with and without calcium ionophore A23187. Platelet activation was evaluated with CD62P and CD63 antibodies, whereas apoptosis with intracellular anti caspase 3-antibody and JC-1 cationic dye. In order to detect PS externalization we used Annexin V by flow cytometry at the beginning, 20th min and 5th hours of the incubation, respectively. There were positive correlations between caspase-3 activation and PS externaization, ∆Ψm depolarization, CD63, and also between PS externaization and CD62P in incubations with A23187 at 5th hours of incubations. These results suggest that there is a relationship between activation and apoptosis in platelets, and platelet activation may progress to platelet apoptos

Anahtar Kelime: Phosphatidylserines Platelet Activation Annexin A5 Blood Platelets Apoptosis

Konular: Hematoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	OZGEN Ü, ÖZEROL E, AMİNCİ M (2007). Relationship between activation and apoptosis in platelets. , 171 - 177.
Chicago	OZGEN ÜNSAL,ÖZEROL Elif,AMİNCİ Mehmet Relationship between activation and apoptosis in platelets. (2007): 171 - 177.
MLA	OZGEN ÜNSAL,ÖZEROL Elif,AMİNCİ Mehmet Relationship between activation and apoptosis in platelets. , 2007, ss.171 - 177.
AMA	OZGEN Ü,ÖZEROL E,AMİNCİ M Relationship between activation and apoptosis in platelets. . 2007; 171 - 177.
Vancouver	OZGEN Ü,ÖZEROL E,AMİNCİ M Relationship between activation and apoptosis in platelets. . 2007; 171 - 177.
IEEE	OZGEN Ü,ÖZEROL E,AMİNCİ M "Relationship between activation and apoptosis in platelets." , ss.171 - 177, 2007.
ISNAD	OZGEN, ÜNSAL vd. "Relationship between activation and apoptosis in platelets". (2007), 171-177.

APA	OZGEN Ü, ÖZEROL E, AMİNCİ M (2007). Relationship between activation and apoptosis in platelets. Turkish Journal of Hematology, 24(4), 171 - 177.
Chicago	OZGEN ÜNSAL,ÖZEROL Elif,AMİNCİ Mehmet Relationship between activation and apoptosis in platelets. Turkish Journal of Hematology 24, no.4 (2007): 171 - 177.
MLA	OZGEN ÜNSAL,ÖZEROL Elif,AMİNCİ Mehmet Relationship between activation and apoptosis in platelets. Turkish Journal of Hematology, vol.24, no.4, 2007, ss.171 - 177.
AMA	OZGEN Ü,ÖZEROL E,AMİNCİ M Relationship between activation and apoptosis in platelets. Turkish Journal of Hematology. 2007; 24(4): 171 - 177.
Vancouver	OZGEN Ü,ÖZEROL E,AMİNCİ M Relationship between activation and apoptosis in platelets. Turkish Journal of Hematology. 2007; 24(4): 171 - 177.
IEEE	OZGEN Ü,ÖZEROL E,AMİNCİ M "Relationship between activation and apoptosis in platelets." Turkish Journal of Hematology, 24, ss.171 - 177, 2007.
ISNAD	OZGEN, ÜNSAL vd. "Relationship between activation and apoptosis in platelets". Turkish Journal of Hematology 24/4 (2007), 171-177.