Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts

Beyatlı, Yavuz; Aslim, Belma

Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts

Belma ASLIM, (Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Ankara Türkiye)

Yavuz BEYATLI (Girilmedi)

Turkish Journal of Veterinary and Animal Sciences

0 0

Yıl: 2004 Cilt: 28 Sayı: 2 Sayfa Aralığı: 257 - 263 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts

Öz:

Türkiye'deki farklı köy ve kasabalardan toplanan yoğurt örneklerinden 34 sus Streptococcus thermophilus olarak teşhis edilmiştir. 34 S. thermophilus susunun antibiyotik dirençlilikleri ve plasmid içerikleri incelenmiştir. Sekiz antibiyotiğe (vankomisin, tetrasiklin, kloramfenikol, eritromisin, sefaletin, sulbaktam ampisilin, gentamisin, ve penisilin G) suşların dirençliliği disk difüzyon metoduna göre incelenmiştir. Bir çok S. thermophilus susunun gentamisin (% 79) ve penisilin G'ye (% 64) dirençlilik gösterdiği belirlenirken, suşların % 88 oranında tetrasikline, % 94 oranında da kloramfenikole duyarlılık gösterdiği tespit edilmiştir. Yapılan plazmid DNA analizlerinde 7 susun plazmid DNA içermediği belirlenmiştir. Plazmid DNA'sı belirlenen suşlarda plazmid DNA sayısının l ve 5 arasında, molekül ağırlıklarının ise 1,88-19,89 kb arasında olduğu tespit edilmiştir. S. thermophilus'un bazı suşlarında antibiyotik dirençiilik ve plazmid içeriği arasında bir etkileşimin olduğu gözlenmiştir. Bir veya hiç plazmide sahip olmayan bazı suşların bir çok antibiyotiğe duyarlı oldukları belirlenmiştir. Beş plazmid DNA'ya sahip 3 susun da bir çok antibiyotiğe dirençli olduğu tespit edilmiştir. Bunun yanısıra bazı suşlarda plazmid DNA içerikleri ile antibiyotik dirençlilikleri arasında korelasyon belirlenememiştir.

Anahtar Kelime: Streptococcus ilaç direnci soy farklılıkları gentamisin Streptococcus thermophilus vankomisin plazmitler gıda bulaşıklılığı tetrasiklin DNA antibiyotikler ampisilin sefalotin eritromisin kloramfenikol

Konular: Ziraat Mühendisliği

Türk yoğurtlarından izole edilen Streptoccus thermophilus suşlarının plazmid DNA içerikleri ve antibiyotik dirençliliği

Öz:

Thirty-four Streptococcus thermophiius strains were isolated from yogurt samples obtained from various villages in different regions of Turkey. These strains were examined for their antibiotic resistance patterns and plasmid carriage. The susceptibility of the isolates to 8 antibiotics (vancomycin, tetracycline, chloramphenicol, erythromycin, cephalothin, ampicillin-sulbactam, gentamicin and penicillin G) was determined by the disk diffusion method. Most strains of S. thermophilus were resistant to gentamicin (79%) and penicillin G (64%) and susceptible to chloramphenicol (94%) and tetracycline (88%). In plasmid DNA analyses, it was determined that 7 strains did not contain any plasmid DNA, while other strains included plasmid DNA ranging from 1 to 5. The molecular weights of these plasmids were found to be in various sizes from 1.88 to 19.89 kb. An interaction was observed between the resistance to antibiotics and the occurrence of plasmids in some S. thermophilus strains; for instance, some strains carrying only one or no plasmid were observed to be susceptible to most antibiotics. The resistance to most antibiotics was determined in 3 strains (B55.S, B56.S, B58.S) containing 5 plasmid DNAs. However, no correlation was observed between the resistance to antibiotics and the occurrence of plasmids in some strains.

Anahtar Kelime: antibiotics ampicillin cefalotin erythromycin chloramphenicol Streptococcus drug resistance strain differences gentamicin Streptococcus thermophilus vancomycin plasmids food contamination tetracycline DNA

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Somkuti, G.A., Steinberg, D.H.: Distribution and analysis of plasmids of Streptococcus thermophilus. J. lnd. Microbiol., 1986; 1, 157-163.
2. Kosikowski, F.; Cheese and Fermented Milks. 2nd ed.; Edwards Broth. Inc. Ann. Arbor: Michigan, 1977.
3. Lewis, J.E.: Cheese Starters: Developments and application of the Lewis System. Elsever Applied Science Publishers: London. 1987.
4. Larsen, R.F., Anon, M.C.: Interaction of antibiotics and water activity on Streptococcus thermophilus and Lactobacillus bulgaricus. J. Food Sci., 1989; 54: 922-923.
5. Herman, R.E., Me Kay, L.L.: Isolation and partial characterization of piasmid DNA from Streptococcus thermophilus. Appl. Environ. Microbiol., 1985; 50: 1103-1106.
6. Me Kay, L.L.: Functional properties of plasmids in lactic streptococci. Anton. Van. Leeuw. J. Microbiol. 1983; 43, 259-274. 7. Miteva, V., Stefanova, T.Z., Takova, T.Z, Grigorova, R.: Isolation and characterisation of plasmids from different strains of Lactobacillus bulgaricus and Streptococcus thermophilus. Acta Microbiol. Bulg., 1991; 27: 3-8.
8. Lykova, E.A.: The antibacterial resistance of the strains making up the components of probiotic preparations. Zh. Microbial. Epidemiol, lmmunobiol., 2000; 2: 63-66.
9. Buchanan, R.E., Gibbons, N.E.: Bergey's Manual of Determinative Bacteriology, Williams and Wilkins Co., Baltimore, 1974.
10. Holdeman, L.V., Cato, E.P., Moore, W.E.C.: Anaerobe Laboratory Manual, 4th ed. Virginia Polytechnic Institute and State University, Blacksbur'g, Virginia, 1975.
11. O'Sullivan, D.J., Klaenhammer, T.R.: Rapid mini prep isolation of high quantity piasmid DNA from Lactococcus and Lactobacillus spp. Appl. Environ. Microbiol., 1993; 59: 2730-2733.
12. Somkuti, G.A., Steinberg, D.H.: General method for piasmid DNA isolation from thermophilic lactic acid bacteria. J. Biotech., 1986; 3: 323-332.
13. Sambrook, J., Fidsch, E.F., Maniatis, T.: Molecular Cloning. 2nd ed., 1989.
14. Murray, P.R., Baron, E.. Pfaller, M.A, Tenover, F.C., Yolken, R.H.: Manual of Clinical Microbiology (Antimicrobial susceptibility tests) 6th ed. ASM, Washington, DC, 1995.
15. Performance Standard for Antimicrobial Disk Susceptibility Tests; Approved Standard-Seventh Edition, January 2000, M2-A7, Vol. 20, No 1, NCCLS.
16. Çetin, E.T., Gürler, N.: Bakterilerin antibiyotiklere duyarlılık deneyinin yapılması. Kükem Derg., 1989; 12: 97-104.
17. Lim, K.S., Huh, C.S., Baek, Y.J.: Studies on the antimicrobial susceptibility of lactic acid bacteria in cultured milk products. Korean J. Dairy Sci., 1995; 17 (In Korean, Abstract in English).
18. Sippel, K., Dulffer-Sceitzer, B., Lammer, C.T.: The characteristic properties of streptococci of serological group. L. Zbl. Vet. Med. B., 1995; 42: 42-50.
19. Charteris, W.P., Kelly, P.M., Morelli, L, Collins, J.K.: Antibiotic susceptibility of potentially probiotic Lactobacillus species. J. Food Prat. 1998; 61: 1636-1643.
20 Lykova, E.A.: Intestinal dysbacteriosis in antibacterial therapy and perspectives in treatment by antibiotic-resistant probiotics. Antibiot Khimioter., 2001; 46: 21-25.
21 Casey, M.G., Jimeno, J.: Lactobacillus delbrübkii subsp. lactis plasmids. Net. Milk Dairy,, 1989; 43: 279-286.
22 Hardy, K.: Aspects of Microbiology: Bacterial Plasmids, Thomas and Nelson Ltd., Hong Kong, 1981.
23. Levy- B.S.: Antibiotics use for^growth promotion in animals. Ecologic and public health consequences. J. Food Protect., 1987; 50: 616-620.
24. Lin, J.H., Savage, D.C.: Genetic transformation of rifampicin resistance in Lactobacillus acidophilus. J. Gen. Microbiol., 1986; 132:2107-2111.
25. Swenson, J.M., Facklam, R.R., Thornsberry, C: Antimicrobial susceptibility of vancomycin resistant Leuconostoc, Pediococcus and Lactobacillus species. Antimicrob. Agents Chemoter., .1990; 34: 543-549.
26. Axelsson, L.T., Ahrne, S.E.I., Andersson, M.C., Stahl, S.R.: Identification and cloning of plasmid-endocoded erythromycin- resistance determinant form Lactobacillus reuteri. Plasmid, 1988; 20: 171-174.
27. Tyurin, M.V., Shenderov, B.A., Grigoryev, A.E., Zhukov, V.G., Navashin, S.M.: Transfer of various markers in Lactobaciiii during plasmid DNA transformation and their joint cultivation. Antibiot. Chemioter., 1990; 35: 16-21.

APA	Aslim B, Beyatlı Y (2004). Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. , 257 - 263.
Chicago	Aslim Belma,Beyatlı Yavuz Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. (2004): 257 - 263.
MLA	Aslim Belma,Beyatlı Yavuz Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. , 2004, ss.257 - 263.
AMA	Aslim B,Beyatlı Y Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. . 2004; 257 - 263.
Vancouver	Aslim B,Beyatlı Y Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. . 2004; 257 - 263.
IEEE	Aslim B,Beyatlı Y "Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts." , ss.257 - 263, 2004.
ISNAD	Aslim, Belma - Beyatlı, Yavuz. "Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts". (2004), 257-263.

APA	Aslim B, Beyatlı Y (2004). Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. Turkish Journal of Veterinary and Animal Sciences, 28(2), 257 - 263.
Chicago	Aslim Belma,Beyatlı Yavuz Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. Turkish Journal of Veterinary and Animal Sciences 28, no.2 (2004): 257 - 263.
MLA	Aslim Belma,Beyatlı Yavuz Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. Turkish Journal of Veterinary and Animal Sciences, vol.28, no.2, 2004, ss.257 - 263.
AMA	Aslim B,Beyatlı Y Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(2): 257 - 263.
Vancouver	Aslim B,Beyatlı Y Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(2): 257 - 263.
IEEE	Aslim B,Beyatlı Y "Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts." Turkish Journal of Veterinary and Animal Sciences, 28, ss.257 - 263, 2004.
ISNAD	Aslim, Belma - Beyatlı, Yavuz. "Antibiotic resistance and plasmid DNA contents of Streptococcus thermophilus strains isolated from Turkish yogurts". Turkish Journal of Veterinary and Animal Sciences 28/2 (2004), 257-263.