The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification

Yurdun, Türkan; ÖZYARAL, Oğuz; DEMIRPEK, UGUR; ÖZYURT, Mustafa; ARDIÇ, Nurittin; YILDIZ, ŞENOL; HAZNEDAROĞLU, Tunçer; Turan, Kadir

The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification

Mustafa ÖZYURT, (GATA Haydarpaşa Eğitim Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Kliniği, İstanbul, Türkiye)

Nurittin ARDIÇ, (GATA Haydarpaşa Eğitim Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Kliniği, İstanbul, Türkiye)

Kadir TURAN, (Marmara Üniversitesi, Eczacılık Fakültesi, İstanbul, Türkiye)

Şenol YILDIZ, (GATA Haydarpaşa Eğitim Hastanesi, İstanbul, Türkiye)

Oğuz ÖZYARAL, (SCA Stars Crasents Assistance, Medical Counseling, İstanbul, Türkiye)

Uğur DEMİRPEK, (GATA Haydarpaşa Eğitim Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Kliniği, İstanbul, Türkiye)

Tunçer HAZNEDAROĞLU, (GATA Haydarpaşa Eğitim Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Kliniği, İstanbul, Türkiye)

Türkan YURDUN (Marmara Üniversitesi, Eczacılık Fakültesi, İstanbul, Türkiye)

Marmara Medical Journal

0 0

Yıl: 2008 Cilt: 21 Sayı: 1 Sayfa Aralığı: 68 - 72 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification

Öz:

Fusarium türleri bağışıklıkları baskılanmış hastalar için önemli fırsatçı patojenlerdir. Bu çalışmada, diyabetik ayaklı bir hastadan izole edilen patojenin, konvansiyonel ve moleküler tekniklerle tanımlanması yapılarak, Fusarium sporotrichioides olduğu sonucuna varıldı. HPLC analizleri, patojenin T-2 toksinini üretmediği görüldü. Dolayısıyla, laboratuvar tanılarında, Fusarium türleri basit çevresel kontaminantlar olarak düşünülmemelidir.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Diyabetik ayaklı bir hastaya ait yara örneğinden fusarium sporotrichioides izolasyonu ve tanımlanması

Öz:

Fusaria are major opportunist pathogens for immunocompromised patients. In this study, it was concluded as a result of both conventional and molecular identification techniques that the isolate obtained from the patient’s diabetic foot was Fusarium sporotrichioides. T-2 toxin production of the pathogen was investigated using HPLC and no toxin determined. In laboratory diagnosis, it must not be thought that Fusaria are solely an environmental contaminant.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Olgu Sunumu Erişim Türü: Erişime Açık

1) Brown DW, McCormick SP, Alexander NJ, Proctor RH, Desjardins AE. Inactivation of a cytochrome P-450 is a determinant of trichotecene diversity in Fusarium species. Fungal Genet Biol 2002; 36: 224-233.
2) Kenedy MJ, Sigler L. Aspergillus, Fusarium, and other opportunistic moniliaceous fungi. In: Murray P, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds., Manuel of Clinical Microbiology, 6th ed. Washington D C: ASM Press, 1995; 765-790.
3) Cenis JL. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 1992; 20: 2380.
4) Wilson A, Simpson D, Chandler E, Jennings P, Nicholson P. Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae. FEMS Microbiol Lett 2004; 233: 69-76.
5) Jimenez M, Mateo JJ, Mateo R. Determination of typeA trichothecenes by determination of typeA trichothecenes by high-performance liquid chromatography with coumarin-3-carbonyl chloride derivatisation and fluorescence detection. J Chromatogr 2000; A 870: 473-481.
6) Jimenez M, Mateo R. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography. J Chromatogr 1997; 778: 363-372.
7) White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ. (eds.), PCR Protocols, San Diego, Academic Press, 1990; 315-322.
8) Waalwijk C, De Koning JRA, Baayen RP, Gams W. Discordant groupings of Fusarium spp. from the sections Elegans, Liseola and Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia 1996; 88; 361-368.
9) Samson RA, Hoekstra ES Oorschot CA. Introduction to food-borne fungi. 2nd ed. CBS, The Nederland. 1984.
10) Mateo JJ, Mateo R, Jimenez M. Accumulation of type A trichothecenes in maize, wheat and rice by F. sporotrichioides isolates under diverse culture conditions. Int J Food Microbiol 2002; 72: 115-123.
11) Bigley VH, Duarte RF, Gosling RD, Kibbler CC, Seaton S, Potter M. Fusarium dimerum infection in a stem cell transplant recipient treated successfully with voriconazole. Bone Marrow Transpl 2004; 34: 815-817.
12) Moschovi M, Trimis G, Anastasopoulos J, Kanariou M, Raftopoulou A, Tzortzatou-Stathopoulou F. Subacute vertebral osteomyelitis in a child with diabetes mellitus associated with Fusarium. Pediatr Int 2004; 46: 740-742.
13) Hemashettar BM, Siddaramappa B, Padhye AA, Sigler L, Chandler FW. Wite grain mycetoma caused by a cylindrocarpon sp. in India. J Clin Microbiol 2000; 38: 4288-4291.
14) Cocuroccia B, Gaido J, Gubinelle E, Annessi G, Girolomoni G. Localized cutaneous hyalohyphomycosis caused by a Fusarium species infection in a renal transplant patient. J Clin Microbiol 2003; 41: 905-907.

APA	ÖZYURT M, ARDIÇ N, Turan K, YILDIZ Ş, ÖZYARAL O, DEMIRPEK U, HAZNEDAROĞLU T, Yurdun T (2008). The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. , 68 - 72.
Chicago	ÖZYURT Mustafa,ARDIÇ Nurittin,Turan Kadir,YILDIZ ŞENOL,ÖZYARAL Oğuz,DEMIRPEK UGUR,HAZNEDAROĞLU Tunçer,Yurdun Türkan The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. (2008): 68 - 72.
MLA	ÖZYURT Mustafa,ARDIÇ Nurittin,Turan Kadir,YILDIZ ŞENOL,ÖZYARAL Oğuz,DEMIRPEK UGUR,HAZNEDAROĞLU Tunçer,Yurdun Türkan The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. , 2008, ss.68 - 72.
AMA	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. . 2008; 68 - 72.
Vancouver	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. . 2008; 68 - 72.
IEEE	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T "The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification." , ss.68 - 72, 2008.
ISNAD	ÖZYURT, Mustafa vd. "The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification". (2008), 68-72.

APA	ÖZYURT M, ARDIÇ N, Turan K, YILDIZ Ş, ÖZYARAL O, DEMIRPEK U, HAZNEDAROĞLU T, Yurdun T (2008). The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal, 21(1), 68 - 72.
Chicago	ÖZYURT Mustafa,ARDIÇ Nurittin,Turan Kadir,YILDIZ ŞENOL,ÖZYARAL Oğuz,DEMIRPEK UGUR,HAZNEDAROĞLU Tunçer,Yurdun Türkan The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal 21, no.1 (2008): 68 - 72.
MLA	ÖZYURT Mustafa,ARDIÇ Nurittin,Turan Kadir,YILDIZ ŞENOL,ÖZYARAL Oğuz,DEMIRPEK UGUR,HAZNEDAROĞLU Tunçer,Yurdun Türkan The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal, vol.21, no.1, 2008, ss.68 - 72.
AMA	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal. 2008; 21(1): 68 - 72.
Vancouver	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification. Marmara Medical Journal. 2008; 21(1): 68 - 72.
IEEE	ÖZYURT M,ARDIÇ N,Turan K,YILDIZ Ş,ÖZYARAL O,DEMIRPEK U,HAZNEDAROĞLU T,Yurdun T "The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification." Marmara Medical Journal, 21, ss.68 - 72, 2008.
ISNAD	ÖZYURT, Mustafa vd. "The isolation of fusarium sporotrichioides from a diabetic foot wound sample and identification". Marmara Medical Journal 21/1 (2008), 68-72.