Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows

BÜLBÜL, Tuğba; AVCI, Gülcan; BÜLBÜL, Aziz; AYDIN, Ibrahim; ÇELİK, Hacı Ahmet

Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows

Hacı Ahmet ÇELİK, (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Afyon, Türkiye)

Gülcan AVCI, (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Afyon, Türkiye)

İbrahim AYDIN, (Selçuk Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Konya, Türkiye)

Aziz BÜLBÜL, (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Afyon, Türkiye)

Tuğba BÜLBÜL (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Hayvan Besleme ve Beslenme Hastalıkları Anabilim Dalı, Afyon, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

6 1

Yıl: 2009 Cilt: 15 Sayı: 1 Sayfa Aralığı: 87 - 94 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows

Öz:

Çalışmada döl tutmayan ineklerde β-karotenin ovaryum fonksiyonları ve gebelik oranı üzerine etkisi araştırıldı. Toplam 26 baş Holstein inek, kontrol (n=11) ve karoten gruplarına (n=15) ayrıldı. İnekler tam yem (kuru maddede %39.80 nötral deterjan fiber ve %16.20 ham protein) rasyon ile beslendi. Karoten grubuna ilk GnRH enjeksiyonu ile aynı zamanda tek doz (1 mg/kg) β-karoten kas içi uygulandı. Prostaglandin enjeksiyon zamanında serum progesteron (P<0.01), β-karoten (P<0.05) ve vitamin A (P<0.01) düzeyleri ile birlikte en büyük follikül (P<0.05) ve korpus luteum çapı (P<0.01) karoten grubunda daha yüksek belirlendi. İkinci GnRH enjeksiyon zamanında da en büyük follikül çapı (P<0.01) ile serum östradiol (P<0.01), β-karoten (P<0.01), vitamin A (P<0.01) düzeyleri karoten grubunda daha yüksek gözlendi. Tohumlama zamanında serum östradiol (P<0.01) ve β-karoten (P<0.05) düzeyinin karoten grubunda yüksek olduğu tespit edildi. Grupların ilk ve 2.GnRH enjeksiyonları sonrasında ovulasyon oranları benzer bulundu. Gebelik oranı kontrol grubunda %27.2, karoten grubunda %33.3 olacak şekilde belirlendi, ancak farkın istatistiki önemde olmadığı gözlendi. Sonuç olarak, β-karotenin döl tutmayan ineklerde folliküllerin çapını etkileyerek plazma östrojen düzeyini artırdığı, korpus luteum çapında artış oluşturarak plazma progesteron düzeyini yükselttiği, ancak ovulasyon ve gebelik oranına etkisinin olmadığı kanısına varıldı.

Anahtar Kelime: rasyon östradiol yumurtalıklar beta-karoten inek gebelik oranları eş zamanlama folikül GnRH diyet A vitamini retinol Alacalı Sığır ovulasyon

Konular: Veterinerlik

Döl tutmayan ineklerde B-karotenin ovaryum fonksiyonları ve ovsynch başarısına etkisi

Öz:

This study was carried out to determine the effect of β-carotene on ovarian function and pregnancy rates in repeat breeder cows. Twenty-six repeat breeder Holstein cows were randomly assigned into control (n=11) and carotene (n=15) groups. Cows were fed with diet in the form of total mixed ration (39.80% neutral detergent fiber and 16.20% crude protein of dry matter). Treatment cows received an intramuscular injection of β-carotene (1 mg/kg) simultaneously with the first GnRH injection. Largest follicle (P<0.05) and corpus luteum diameters (P<0.01) together with serum progesterone (P<0.01), β-carotene (P<0.05) and vitamin A (P<0.01) levels were all significantly higher in the carotene group on the day of PGF2α injection. At the second GnRH injection, largest follicle diameter (P<0.01), serum estradiol (P<0.01), β-carotene (P<0.01), and vitamin A (P<0.01) levels were again higher in the carotene group. Serum estradiol (P<0.01) and β-carotene (P<0.05) levels were still significantly higher in the carotene group at the time of AI. Ovulation response did not differ between groups after both first and second GnRH injection. The pregnancy rate was 27.2% in the control group, and 33.3% in the carotene group but this difference was not statistically significant. In conclusion, it was suggested that β-carotene did not have any significant effects on ovulation or pregnancy rates in ovsynch-treated repeat breeder cows; however it increased serum estradiol and progesterone levels due to increased follicle size and corpus luteum functionality, respectively.

Anahtar Kelime: vitamin A retinol Holstein-Friesian ovulation ration estradiol ovaries beta-carotene cows pregnancy rate synchronization follicles GnRH diet

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Selvaraju S, Agarwal SK, Karche SD, Srivastava SK, Majumdar AC, Shanker U: Fertility responses and hormonal profiles in repeat breeding cows treated with insulin. Anim Reprod Sci, 73,141–149, 2002.
Peters AR: Embryonic mortality in the cow. Anim Breed Abstr, 64, 587–598, 1996.
Bage R, Gustafsson H, Larsson B, Forsberg M, Rodriguez- Martinez H: Repeat breeding in dairy heifers: follicular dynamics and oestrous cycle characteristics in relation to sexual hormone patterns. Theriogenology, 5, 2257-2269,2002.
Goley RR, Kadu MS: Efficacy of prostaglandin F2, Lutalyse, GnRH analogue, Receptal and hCG, Chorulon in treatment of repeat breeder cows. Indian Vet J, 72, 472–475, 1995.
Weiss WP: Requirements of fat-soluble vitamins for dairy cows: A review. J Dairy Sci, 81, 2493–2501, 1998.
Schweigert FJ, Lutterbach A, Rambeck WA, Zucker H: Vitamin A and β-carotene concentrations in bovine follicular fluid in relationship to follicle size. J Vet Med A, 33, 360, 1986.
Perret BP, Parinaud J, Ribbes H, Moatti JP, Pontonnier G, Chap H, Douste-Blazy L: Lipoprotein and phospholipid distribution in human follicular fluid. Fert Steril, 43, 405–409, 1985.
Schweigert FJ, Zucker H: Concentrations of vitamin A, beta-carotene and vitamin E in individual bovine follicles of different quality. J Reprod Fertil, 82, 575–579, 1988.
Brief S, Chew BP: Effects of vitamin A and β-carotene on reproductive performance in gilts. J Anim Sci, 60,998–1004, 1985.
Aslan S, Handler J, Arbeiter K: Frühgraviditat und embryonale bzw. frühfetale Mortalität bei der Kuh- Gelbkörperdynamik, Progesteron-, Vitamin E-, Vitamin B12-, β-carotin- und Folsäurekonzentrationen im peripheren Blut. Wien Tierarztl Mschr, 85, 141–147 1998.
Arikan S, Rodway RG: Seasonal variation in bovine luteal concentrations of beta-carotene. Turk J Vet Anim Sci, 25, 165-168, 2001.
Haliloglu S, Baspinar N, Serpek B, Erdem H, Bulut Z: Vitamin A and β-carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reprod Dom Anim, 37, 96–99, 2002.
Lotthammer KH: Importance of β-carotene for the fertility of dairy cattle. Feedstuffs, 51, 6, 1979.
Kaçar C, Kamiloğlu NN, Uçar Ö, Arı UÇ, Pancarcı ŞM, Güngör Ö: İneklerde β-karoten + E vitamini uygulamasıyla kombine edilen ovsynch ve cosynch senkronizasyon programlarının gebelik oranı üzerine etkisi. Kafkas Üniv Vet Fak Derg, 14 (1): 45-50, 2008.
National Research Council: Nutrient requirements of dairy cattle. Seventh Revised Edition. National Academic Press, Washington, DC.,USA, 2001.
Suziki J, Katoh N: A simple and cheap methods for measuring serum vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci, 52, 1281-1283, 1990.
Arechiga CF, Vazquez-Flores S, Ortiz O, Hernandez- Ceron J, Porras A, McDowell LR, Hansen PJ: Effect of injection of β-carotene or vitamin E and selenium on fertility of lactating dairy cows. Theriogenology, 50, 65-76, 1998.
Kaneko H, Watanabe G, Taya K, Sasamoto S, Hasegawa Y: Changes in plasma concentration of immunoreactive inhibin, estradiol and FSH associated with follicular waves during the estrous cycle of the cow. J Reprod Dev, 41, 311-320, 1995.
Maenpaa PH, Pirhonen A, Koskinen E: Vitamin A, E and D nutrition in mares and foals during the winter seasons; effects of feeding two different vitamin-mineral concentrate. J Anim Sci, 66, 1424–1429, 1988.
Brown JA, Eberhardt DM, Schrick FN, Roberts MP, Godkin JD: Expression of retinol binding protein and cellular retinol-binding protein in the bovine ovary. Mol Reprod Dev, 64, 261–269, 2003.
Rapaport R, Sklan D, Wolfenson D, Shaham-Albalancy A, Hanukoglu I: Antioxidant capacity is correlated with steroidogenic status of the corpus luteum during the bovine estrous cycle. Biochim Biophys Acta, 1380, 133–140, 1998.
Ahlswede L, Lotthammer KH: Untersuchungen uber eine spezifische, vitamin A-unabhangige wirkung des β-carotins auf die fertilitat des rindes. 5. Mitt. Organunterschungen- Gewichts und Gehaltsbestimmungen. Dtch Tierarztl Wochenschr, 85, 7–12, 1978.
Chew BP, Holpuch DM, O’Fallon JV: Vitamin A and bcarotene in bovine and porcine plasma, liver, corpora lutea, and follicular fluid. J Dairy Sci, 67, 1316–1322, 1984.
Graves-Hoagland RL, Hoagland TA, Woody CO: Relationship of plasma β-carotene and vitamin A to luteal function in postpartum cattle. J Dairy Sci, 72, 1854–1858,1989.
Talavera F, Chew BP: Comparative role of retinol, retinoic acid and β-carotene on progesterone secretion by pig corpus luteum in vitro. J Reprod Fertil, 82, 611–615, 1987.
Pursley JR, Mee MO, Wiltbank MC: Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 44, 915-923, 1995.
Vasconcelos JLM, Silcox RW, Rosa GLM, Pursley JR, Wiltbank MC: Synchronization rate, size of the ovulatory follicle, and pregnancy rate after synchronization on different days of the estrus cycle in lactating dairy cows. Theriogenology, 52, 1067–1078, 1999.
Kasimanickam R, Cornwell JR, Nebel RL: Fertility following fixed-time AI or insemination at observed estrus in ovsynch and heatsynch programs in lactating dairy cows. Theriogenology, 63, 2550-2559, 2005.
Roberts GP: Structural on the gylicoproteins from bovine cervical mucus. Biochem J, 173, 941-947, 1978.
Chan VT, Wolf G: The role of vitamin A in the glycosylation reactions of glycoprotein synthesis in an in vitro system. Biochem J, 247, 53-62, 1987.
Bello NM, Steibel JP, Pursley JR: Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of ovsynch in lactating dairy cows. J Dairy Sci, 89, 3413-3424, 2006.
Bleach E, Glencross CRG, Knight PG: Association between ovarian follicle development and pregnancy rates in dairy cows undergoing spontaneous oestrous cycles. Reproduction, 127, 621–629, 2004.
Burns DS, Jimenez-Krassel F, Ireland J, Knight PG, Ireland JJ: Numbers of antral follicles during follicular waves in cattle: Evidence for high variation among animals, very high repeatability in individuals, and an inverse association with serum-follicle-stimulating hormone concentrations. Biol Reprod, 73, 54–62, 2005.
Sartori R, Haughian JM, Shaver RD, Rosa GJ, Wiltbank MC: Comparison of ovarian function and circulating steroids in estrous cycles of Holstein heifers and lactating cows. J Dairy Sci, 87, 905–920, 2004.
Souza AH, Gumen A, Silva EPB, Cunha AP, Guenther JN, Peto CM, Caraviello DZ, Wiltbank MC: Supplementation with estradiol-17β before the last Gonadotropin-releasing hormone of the Ovsynch protocol in lactating dairy cows. J Dairy Sci, 90, 4623-34, 2007.
Perez CC, Rodriguez I, Espana F, Dorado J, Hidalgo M, Sanz J: Follicular growth patterns in repeat breeder cows. Ved Med Czech, 48, 1-8, 2003.

APA	ÇELİK H, AVCI G, AYDIN I, BÜLBÜL A, BÜLBÜL T (2009). Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. , 87 - 94.
Chicago	ÇELİK Hacı Ahmet,AVCI Gülcan,AYDIN Ibrahim,BÜLBÜL Aziz,BÜLBÜL Tuğba Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. (2009): 87 - 94.
MLA	ÇELİK Hacı Ahmet,AVCI Gülcan,AYDIN Ibrahim,BÜLBÜL Aziz,BÜLBÜL Tuğba Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. , 2009, ss.87 - 94.
AMA	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. . 2009; 87 - 94.
Vancouver	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. . 2009; 87 - 94.
IEEE	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T "Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows." , ss.87 - 94, 2009.
ISNAD	ÇELİK, Hacı Ahmet vd. "Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows". (2009), 87-94.

APA	ÇELİK H, AVCI G, AYDIN I, BÜLBÜL A, BÜLBÜL T (2009). Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15(1), 87 - 94.
Chicago	ÇELİK Hacı Ahmet,AVCI Gülcan,AYDIN Ibrahim,BÜLBÜL Aziz,BÜLBÜL Tuğba Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15, no.1 (2009): 87 - 94.
MLA	ÇELİK Hacı Ahmet,AVCI Gülcan,AYDIN Ibrahim,BÜLBÜL Aziz,BÜLBÜL Tuğba Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.15, no.1, 2009, ss.87 - 94.
AMA	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(1): 87 - 94.
Vancouver	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(1): 87 - 94.
IEEE	ÇELİK H,AVCI G,AYDIN I,BÜLBÜL A,BÜLBÜL T "Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15, ss.87 - 94, 2009.
ISNAD	ÇELİK, Hacı Ahmet vd. "Effect of B - carotene on ovarium functions and ovsynch success in repeat breeder cows". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15/1 (2009), 87-94.