Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease

BOZDAYI, A. Mithat; KARATAYLI, Özen Senem Ceren; SAPMAZ, Ayşegül; ÖZDEN, Ali; TÖRÜNER, Murat; ÖZKAN, Muhip; ÜLKER, Aysel; Çetinkaya, Hülya; Celik Altunoglu, Yasemin; KILIÇ, Z. Mesut Yalın; SOYKAN, ARİF İRFAN; DAĞLI, Ülkü

Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease

Ayşegül SAPMAZ, (Ankara Üniversitesi, Biyoteknoloji Enstitüsü, Ankara, Türkiye)

Özen Senem Ceren KARATAYLI, (Ankara Üniversitesi, Biyoteknoloji Enstitüsü, Ankara, Türkiye)

Ülkü DAĞLI, (Türkiye Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Gastroenteroloji Kliniği, Ankara, Türkiye)

Z. Mesut Yalın KILIÇ, (Türkiye Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Gastroenteroloji Kliniği, Ankara, Türkiye)

Murat TÖRÜNER, (Ankara Üniversitesi, Tıp Fakültesi, Gastroenteroloji Anabilim Dalı, Ankara, Türkiye)

YASEMİN ÇELİK ALTUNOĞLU, (Ankara Üniversitesi, Biyoteknoloji Enstitüsü, Ankara, Türkiye)

Muhip ÖZKAN, (Ankara Üniversitesi, Ziraat Fakültesi, Genetik ve Biometri Anabilim Dalı, Ankara, Türkiye)

ARİF İRFAN SOYKAN, (Ankara Üniversitesi, Tıp Fakültesi, Gastroenteroloji Anabilim Dalı, Ankara, Türkiye)

Hülya ÇETİNKAYA, (Ankara Üniversitesi, Tıp Fakültesi, Gastroenteroloji Anabilim Dalı, Ankara, Türkiye)

Aysel ÜLKER, (Türkiye Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Gastroenteroloji Kliniği, Ankara, Türkiye)

Ali ÖZDEN, (Ankara Üniversitesi, Tıp Fakültesi, Gastroenteroloji Anabilim Dalı, Ankara, Türkiye)

A. Mithat BOZDAYI, (Ankara Üniversitesi, Biyoteknoloji Enstitüsü, Ankara, Türkiye)

Diğer Yazarlar

Turkish Journal of Gastroenterology

2 1

Yıl: 2008 Cilt: 19 Sayı: 3 Sayfa Aralığı: 168 - 173 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease

Öz:

Amaç: Chron ve ülseratif kolit temel nedeni tam olarak bilinmeyen gastrointestinal sistemin kronik hastalıklarıdır. Ülseratif kolit ve Crohn hastalığı intestinal lümende yaşayan bakterilere karşı hücre aracılı immün cevap ile karakterize edilir. Barsağı n ksenobiyotiklere ve bakteri toksinlerine karşı korunması nda, MDR1 geni tarafından kodlanan P-glikoproteininin fonksiyonunun ve ekspresyon seviyesinin önemli olduğu ileri sürülmektedir. Bu nedenle MDR1 genindeki mutasyonların inflamatuvar barsak hastalığı patogenezi ile ilişkili olduğu düşünülmektedir. Bu çalışmanın amacı MDR1 geninde görülen G2677T/A polimorfizminin Türk inflamatuvar barsak hastaları ve sağlıklı bireylerde incelenmesidir. Yöntem: Çalışmamı zda endoskopik veya histopatolojik olarak tanı almış olan 35 Crohn (14 kadın + 21 erkek), 82 (36 kadın + 46 erkek) ülseratif kolit hastasının ve 70 (39 kadın + 31 erkek) sağlıklı bireyin genotipleri karşılaştırılmıştır. Bu çalışmada aday bölgedeki polimorfizmleri (G2677T ve G2677A) saptamak için polimeraz zincir reaksiyonu-restriksiyon parçacığı uzunluk polimorfizmi yöntemi kullanılmıştır. Bulgular: Bu çalışmada, MDR1 geninin triallelik bir polimorfizm taşıyan 2677 pozisyonundaki allel frekansı hasta ve sağlıklı kontrol grupları arasında anlamlı bir farklılık göstermemiştir. Ayrıca 2677A alleline her iki grupta da rastlanılmamıştır. Sonuç: Bu çalışmada, MDR1 geninde incelenen G2677T/A polimorfizmi Crohn veya ülseratif kolit için risk faktörü olarak bulunmamıştır.

Anahtar Kelime: Polimorfizm, tek nükleotid Ki-kare dağılımı Genotip Polimeraz zincir reaksiyonu Polimorfizm, kesim parçacığı uzunluk Olgu-kontrol çalışmaları Enflamatuvar bağırsak hastalıkları P-glikoprotein

Konular: Cerrahi

MDR1 geni G2677T/A triallelik bölgesi polimorfizminin inflamatuvar barsak hastalığı olan Türk hastalarındaki etkisi

Öz:

Background/aims: Crohn’s disease and ulcerative colitis are both chronic inflammatory disorders of the gastrointestinal tract, the main causes of which remain unknown. Crohn’s disease and ulcerative colitis are characterized by cell-mediated immune response against the luminal bacteria. It is suggested that expression levels and function of P-glycoprotein, encoded by the MDR1 gene, are important for protection of the gut against xenobiotics and bacterial toxins. Therefore, the mutations of the MDR1 gene are thought to be related with the pathogenesis of inflammatory bowel disease. The aim of this study was to investigate the G2677T/A polymorphism in the MDR1 gene in Turkish patients with inflammatory bowel disease and a healthy control group. Methods: In our study, the genotypes of endoscopically or histopathologically diagnosed Crohn’s disease (n: 35; 14 F, 21 M) and ulcerative colitis (n: 82; 36 F, 46 M) patients and of 70 healthy individuals (39 F, 31 M) were compared. In the patient and control groups, polymerase chain reaction- restriction fragment length polymorphism analysis was performed for two polymorphisms (G2677T and G2677A) of the MDR1 gene. Results: In this study, the frequency of alleles at position 2677 of the MDR1 gene, which has a triallelic polymorphism, was not found to be significantly different between the patient and the healthy control groups. Moreover, the 2677A allele was not detected in either the patient group or the healthy control group. Conclusions: In this study, the G2677T/A polymorphism observed in the MDR1 gene was not found to be a risk factor for Crohn’s disease or ulcerative colitis.

Anahtar Kelime: Case-Control Studies Inflammatory Bowel Diseases P-Glycoprotein Polymorphism, Single Nucleotide Chi-Square Distribution Genotype Polymerase Chain Reaction Polymorphism, Restriction Fragment Length

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SAPMAZ A, KARATAYLI Ö, DAĞLI Ü, KILIÇ Z, TÖRÜNER M, Celik Altunoglu Y, ÖZKAN M, SOYKAN A, Çetinkaya H, ÜLKER A, ÖZDEN A, BOZDAYI A (2008). Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. , 168 - 173.
Chicago	SAPMAZ Ayşegül,KARATAYLI Özen Senem Ceren,DAĞLI Ülkü,KILIÇ Z. Mesut Yalın,TÖRÜNER Murat,Celik Altunoglu Yasemin,ÖZKAN Muhip,SOYKAN ARİF İRFAN,Çetinkaya Hülya,ÜLKER Aysel,ÖZDEN Ali,BOZDAYI A. Mithat Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. (2008): 168 - 173.
MLA	SAPMAZ Ayşegül,KARATAYLI Özen Senem Ceren,DAĞLI Ülkü,KILIÇ Z. Mesut Yalın,TÖRÜNER Murat,Celik Altunoglu Yasemin,ÖZKAN Muhip,SOYKAN ARİF İRFAN,Çetinkaya Hülya,ÜLKER Aysel,ÖZDEN Ali,BOZDAYI A. Mithat Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. , 2008, ss.168 - 173.
AMA	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. . 2008; 168 - 173.
Vancouver	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. . 2008; 168 - 173.
IEEE	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A "Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease." , ss.168 - 173, 2008.
ISNAD	SAPMAZ, Ayşegül vd. "Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease". (2008), 168-173.

APA	SAPMAZ A, KARATAYLI Ö, DAĞLI Ü, KILIÇ Z, TÖRÜNER M, Celik Altunoglu Y, ÖZKAN M, SOYKAN A, Çetinkaya H, ÜLKER A, ÖZDEN A, BOZDAYI A (2008). Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. Turkish Journal of Gastroenterology, 19(3), 168 - 173.
Chicago	SAPMAZ Ayşegül,KARATAYLI Özen Senem Ceren,DAĞLI Ülkü,KILIÇ Z. Mesut Yalın,TÖRÜNER Murat,Celik Altunoglu Yasemin,ÖZKAN Muhip,SOYKAN ARİF İRFAN,Çetinkaya Hülya,ÜLKER Aysel,ÖZDEN Ali,BOZDAYI A. Mithat Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. Turkish Journal of Gastroenterology 19, no.3 (2008): 168 - 173.
MLA	SAPMAZ Ayşegül,KARATAYLI Özen Senem Ceren,DAĞLI Ülkü,KILIÇ Z. Mesut Yalın,TÖRÜNER Murat,Celik Altunoglu Yasemin,ÖZKAN Muhip,SOYKAN ARİF İRFAN,Çetinkaya Hülya,ÜLKER Aysel,ÖZDEN Ali,BOZDAYI A. Mithat Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. Turkish Journal of Gastroenterology, vol.19, no.3, 2008, ss.168 - 173.
AMA	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. Turkish Journal of Gastroenterology. 2008; 19(3): 168 - 173.
Vancouver	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease. Turkish Journal of Gastroenterology. 2008; 19(3): 168 - 173.
IEEE	SAPMAZ A,KARATAYLI Ö,DAĞLI Ü,KILIÇ Z,TÖRÜNER M,Celik Altunoglu Y,ÖZKAN M,SOYKAN A,Çetinkaya H,ÜLKER A,ÖZDEN A,BOZDAYI A "Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease." Turkish Journal of Gastroenterology, 19, ss.168 - 173, 2008.
ISNAD	SAPMAZ, Ayşegül vd. "Effects of polymorphism in G2677T/A triallelic region of MDR1 gene in Turkish patients with inflammatory bowel disease". Turkish Journal of Gastroenterology 19/3 (2008), 168-173.