Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease

ÇITIL, MEHMET; ERGİNSOY, Serpil; Tunca, Recai; UZLU, Erdoğan; Sozmen, Mahmut; ERDOĞAN, H.Metin; Devrim, Alparslan Kadir

Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease

Recai TUNCA, (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Aydın, Türkiye)

H.Metin ERDOĞAN, (Kafkas Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Kars, Türkiye)

Mahmut SÖZMEN, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Mehmet ÇİTİL, (Kafkas Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Kars, Türkiye)

Alparslan Kadir DEVRİM, (Kafkas Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kars, Türkiye)

Serpil ERGİNSOY, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Erdoğan UZLU (Kafkas Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Kars, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

7 0

Yıl: 2009 Cilt: 33 Sayı: 1 Sayfa Aralığı: 53 - 59 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease

Öz:

Hayvanlardaki beyaz kas hastalığı (WMD) iskelet ve kalp kasında dejenerasyonla karakterize oldukça yaygın nutrisyonel bir hastalıktır. Çalışmada WMD tanısı konulan (n = 15) ve klinik olarak sağlıklı (n = 8) kuzuların kalp kasındaki kardiyak troponin I (cTnI) ve indüklenebilir nitric oksit sentetaz (iNOS) ekspresyonları araştırıldı. Kreatin kinaz (CK), kreatin kinaz MB (CK-MB), aspartat aminotransferaz (AST) ve laktat dehidrogenaz (LDH) aktiviteleri de her iki grupta değerlendirildi. Ayrıca serum cTnI seviyeleri de ticari ELISA kiti ile belirlendi. WMD olgularında ortalama serum cTnI (10,49 ± 0,25 ng/ml), CK (261,16 ± 8,61 U/l), CK-MB (214,16 ± 12,71 U/l), AST (125,83 ± 1,05) ve LDH (228,77 ± 14,86 U/l) konsantrasyonu yüksekti. İmmunohistokimyasal veriler tüm WMD olgularında, dokudaki cTnI ekspresyonunda belirgin azalmanın şekillendiğine işaret ederken kontrol olarak kullanılan 8 kuzunun hiçbirinde cTnI ekspresyonunda azalmaya rastlanmadı. Bununla beraber iNOS immunoreaktivitesi dejenere kardiyak miyositlerde ve intersitisyumdaki makrofajlarda kontrol olgularına göre oldukça yoğundu. Sonuçlar cTnI’nın miyokardiyal hasarın ortaya konulmasında oldukça duyarlı olarak kullanılabileceğini gösterdi. WMD’nin kardiyak miyositlerde iNOS ekspresyonunu ise arttırdığını ortaya koydu.

Anahtar Kelime: beslenme bozuklukları troponin nitrik oksit sentaz kas hastalıkları kuzu enzim aktivitesi kas distrofisi immünohistokimya

Konular: Ziraat Mühendisliği

Kuzulardaki beyaz kas hastalığında kardiyak troponin I ve indüklenebilir nitrik oksit sentetaz ekspresyonunun değerlendirilmesi

Öz:

White muscle disease (WMD) is a world-wide nutritional disease of all animal species characterised by degeneration of skeletal and cardiac muscles. In the present study we determined the cardiac troponin I (cTnI) and inducible nitric oxide synthase (iNOS) expression in hearts of the lambs with WMD (n = 15). Eight clinically healthy lambs served as control. Creatine kinase (CK), creatine kinase MB (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analysed for both groups. cTnI levels were measured by a commercially available ELISA kit. Mean cTnI (10.49 ± 0.25 ng/ml), CK (261.16 ± 38.61 U/l), CK-MB (214.16 ± 12.71 U/l), AST (125.83 ± 1.05), and LDH (228.77 ± 14.86 U/l) concentrations were higher in WMD cases. Immunohistochemistry revealed definitive loss of cTnI expression in all cases. None of the 8 controls showed loss of the cTnI expression. However, iNOS immunoreactivity was augmented in degenerated cardiac myocytes and macrophages infiltrating to the interstitium compared to controls. The results of the study suggested that cTnI antibody might successfully be used as a sensitive test in the diagnosis of myocardial injury. iNOS expression augments in cardiac myocytes in lambs with WMD, most likely through influencing the signalling pathways to iNOS induction.

Anahtar Kelime: lambs enzyme activity muscular dystrophy immunohistochemistry nutritional disorders troponins nitric oxide synthase muscular diseases

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Tunca R, ERDOĞAN H, Sozmen M, ÇITIL M, Devrim A, ERGİNSOY S, UZLU E (2009). Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. , 53 - 59.
Chicago	Tunca Recai,ERDOĞAN H.Metin,Sozmen Mahmut,ÇITIL MEHMET,Devrim Alparslan Kadir,ERGİNSOY Serpil,UZLU Erdoğan Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. (2009): 53 - 59.
MLA	Tunca Recai,ERDOĞAN H.Metin,Sozmen Mahmut,ÇITIL MEHMET,Devrim Alparslan Kadir,ERGİNSOY Serpil,UZLU Erdoğan Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. , 2009, ss.53 - 59.
AMA	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. . 2009; 53 - 59.
Vancouver	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. . 2009; 53 - 59.
IEEE	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E "Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease." , ss.53 - 59, 2009.
ISNAD	Tunca, Recai vd. "Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease". (2009), 53-59.

APA	Tunca R, ERDOĞAN H, Sozmen M, ÇITIL M, Devrim A, ERGİNSOY S, UZLU E (2009). Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences, 33(1), 53 - 59.
Chicago	Tunca Recai,ERDOĞAN H.Metin,Sozmen Mahmut,ÇITIL MEHMET,Devrim Alparslan Kadir,ERGİNSOY Serpil,UZLU Erdoğan Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences 33, no.1 (2009): 53 - 59.
MLA	Tunca Recai,ERDOĞAN H.Metin,Sozmen Mahmut,ÇITIL MEHMET,Devrim Alparslan Kadir,ERGİNSOY Serpil,UZLU Erdoğan Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences, vol.33, no.1, 2009, ss.53 - 59.
AMA	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(1): 53 - 59.
Vancouver	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(1): 53 - 59.
IEEE	Tunca R,ERDOĞAN H,Sozmen M,ÇITIL M,Devrim A,ERGİNSOY S,UZLU E "Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease." Turkish Journal of Veterinary and Animal Sciences, 33, ss.53 - 59, 2009.
ISNAD	Tunca, Recai vd. "Evaluation of Cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease". Turkish Journal of Veterinary and Animal Sciences 33/1 (2009), 53-59.