Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats

gürer, ahmet; GOMCELI, Ismail; ÖZDOĞAN, Mehmet; AYDIN, Raci; GÖKAKIN, Ali Kağan; Gülbahar, Özlem; ARIKÖK, Ata Türker; KUlacoglu, Hakan

Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats

Ahmet GÜRER, (Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Mehmet ÖZDOĞAN, (Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Ali Kağan GÖKAKIN, (Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

İsmail GÖMCELİ, (Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Özlem GÜLBAHAR, (Gazi Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Ata Türker ARIKÖK, (Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Patoloji Kliniği, Ankara, Türkiye)

Hakan KULAÇOĞLU, (Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Patoloji Kliniği, Ankara, Türkiye)

Raci AYDIN (Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2009 Cilt: 15 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats

Öz:

Travma hastalarında ikinci darbenin oluşması enflamatuvar yanıtın şiddetlenmesine ve çoklu organ gelişmesine neden olmaktadır. Yanık hasarı sonrası oluşan enfeksiyon iki darbeli travma çalışmaları için uygun bir modeldir. Bu çalışmada, ardışık yanık ve sepsisten oluşan iki darbeli travma modelinde N-asetilsistein’in (NAS) etkinliği araştırıldı. GEREÇ VE YÖNTEM: Kırk beş sıçanda %30’luk oranda haşlanma yanığı oluşturulduktan 72 saat sonra çekal bağlama ve delme yöntemi ile peritonit oluşturuldu. Grup I’de herhangi bir tedavi uygulanmadı. Grup II’deki sıçanlara peritonit sonrasında 150 mg/kg/gün dozunda intraperitoneal NAS tedavisi verildi. Grup III’e yanık sonrası 6 gün boyunca 150 mg/kg/gün dozunda NAS tedavisi uygulandı. Yedinci gün doku malondialdehit (MDA) ve glutatyon (GHS) düzeylerinin tayini ve histopatolojik hasarın değerlendirilmesi için örnekler toplandı. BULGULAR: Gruplar arasında mortalite farkı yoktu. Tedavi gruplarında karaciğer ve ileum doku MDA düzeylerinde anlamlı azalma (p=0,01, p=0,02) saptandı. Grup II’de akciğer GSH düzeylerinin anlamlı olarak arttığı (p=0,02) ve akciğer dokusunda histopatolojik hasar skorunun azaldığı saptandı (p=0,005). SONUÇ: N-asetilsistein tedavisi çalışılan iki darbeli travma modelinde doku oksidatif stres düzeyini ve uzak organ hasarını hafifletmiştir. Bu konuda daha ileri deneysel ve klinik çalışmalara ihtiyaç vardır.

Anahtar Kelime: Asetilsistein Akciğer Oksidatif stres Hastalık modelleri, hayvan Glutatyon Antioksidanlar Bağlama Malondialdehit Erkek Yanıklar Karaciğer Modeller, hayvan Rastgele seçim Sıçan, Wistar Çekum Bağırsak delinmesi

Konular: Cerrahi

Ardışık yanık ve peritoneal sepsisle oluşturulan iki darbeli travma modelinde, N-asetilsistein tedavisi doku oksidatif stres düzeyini ve uzak organ hasarını azaltır

Öz:

BACKGROUND: The second hit in trauma leads to an exaggerated inflammatory response and multiple organ failure. Infection following burn injury is a useful model for two-hit trauma studies. The aim of this study was to investigate the effect of N-acetylcysteine (NAC) treatment as an antioxidant in a two-hit trauma model. METHODS: 30% scalding burn injury was performed in 45 rats and cecal ligation-puncture (CLP) was performed 72 hours later. Groups were allocated as follows: Group I: No treatment was performed; Group II: Rats were treated with 150 mg/kg/day i.p. NAC for 72 h following CLP; Group III: Rats were treated with 150 mg/kg/day i.p. NAC for 6 days following thermal injury. Tissue samples were collected to study the tissue malonyldialdehyde (MDA) and glutathione (GSH) levels, and for histopathological examination on day 7. RESULTS: No difference in mortality between groups was detected. Tissue MDA levels significantly decreased in the liver (p=0.01, p=0.02) and ileum (p=0.01, p=0.02) in the treatment groups. Lung tissue GSH levels were found to be significantly increased in Group II (p=0.02). Lung injury scores were decreased in Group II (p=0.005) compared to the control group. CONCLUSION: NAC attenuated tissue oxidative stress level and remote organ injury in two-hit trauma. Further experimental and clinical studies on this subject are necessary.

Anahtar Kelime: Ligation Malondialdehyde Male Burns Liver Models, Animal Random Allocation Rats, Wistar Cecum Intestinal Perforation Acetylcysteine Lung Oxidative Stress Disease Models, Animal Glutathione Antioxidants

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	gürer a, ÖZDOĞAN M, GÖKAKIN A, GOMCELI I, Gülbahar Ö, ARIKÖK A, KUlacoglu H, AYDIN R (2009). Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. , 1 - 6.
Chicago	gürer ahmet,ÖZDOĞAN Mehmet,GÖKAKIN Ali Kağan,GOMCELI Ismail,Gülbahar Özlem,ARIKÖK Ata Türker,KUlacoglu Hakan,AYDIN Raci Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. (2009): 1 - 6.
MLA	gürer ahmet,ÖZDOĞAN Mehmet,GÖKAKIN Ali Kağan,GOMCELI Ismail,Gülbahar Özlem,ARIKÖK Ata Türker,KUlacoglu Hakan,AYDIN Raci Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. , 2009, ss.1 - 6.
AMA	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. . 2009; 1 - 6.
Vancouver	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. . 2009; 1 - 6.
IEEE	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R "Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats." , ss.1 - 6, 2009.
ISNAD	gürer, ahmet vd. "Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats". (2009), 1-6.

APA	gürer a, ÖZDOĞAN M, GÖKAKIN A, GOMCELI I, Gülbahar Ö, ARIKÖK A, KUlacoglu H, AYDIN R (2009). Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Ulusal Travma ve Acil Cerrahi Dergisi, 15(1), 1 - 6.
Chicago	gürer ahmet,ÖZDOĞAN Mehmet,GÖKAKIN Ali Kağan,GOMCELI Ismail,Gülbahar Özlem,ARIKÖK Ata Türker,KUlacoglu Hakan,AYDIN Raci Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Ulusal Travma ve Acil Cerrahi Dergisi 15, no.1 (2009): 1 - 6.
MLA	gürer ahmet,ÖZDOĞAN Mehmet,GÖKAKIN Ali Kağan,GOMCELI Ismail,Gülbahar Özlem,ARIKÖK Ata Türker,KUlacoglu Hakan,AYDIN Raci Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Ulusal Travma ve Acil Cerrahi Dergisi, vol.15, no.1, 2009, ss.1 - 6.
AMA	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2009; 15(1): 1 - 6.
Vancouver	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2009; 15(1): 1 - 6.
IEEE	gürer a,ÖZDOĞAN M,GÖKAKIN A,GOMCELI I,Gülbahar Ö,ARIKÖK A,KUlacoglu H,AYDIN R "Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats." Ulusal Travma ve Acil Cerrahi Dergisi, 15, ss.1 - 6, 2009.
ISNAD	gürer, ahmet vd. "Tissue oxidative stress level and remote organ injury in two-hit trauma model of sequential burn injury and peritoneal sepsis are attenuated with N-acetylcysteine treatment in rats". Ulusal Travma ve Acil Cerrahi Dergisi 15/1 (2009), 1-6.