Yıl: 2008 Cilt: 25 Sayı: 4 Sayfa Aralığı: 172 - 175 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo

Öz:
Amaç: Yeşil floresan proteini (YFP), günümüzde hücre biyolojisi çalışmalarında tanımlayıcı gen ve hücre işaretleyici olarak kullanılmaktadır. YFP’nin oldukça önemli kullanım alanları farklı genlerin içerisine eklenerek bu genlerin farklı organizmalardaki ekspresyonlarının miktar tayininde ve canlı hücreler içerisinde işaretleyici olarak kullanılabilmesidir. Bu çalışmamızda doku tamiri amacıyla ve hayvanlara aktardığımız mezankimal kök hücrelerini (MKH) in vivo takip edebilmek amacı ile YFP genini içeren plazmid vektörünün MKH’lara aktarılmasını optimize etmeye çalıştık. Yöntem ve Gereçler: Bu amaçla YFP geni taşıyan phM-YFP plazmid vektörü ve MKH’lara plazmid vektörün aktarılması amacı ile Effectene Transfeksiyon kiti kullanılmıştır.Bulgular: Elde edilen sonuçlar, MKH’ların phM-YFP ile iki defa transfekte edilmelerinin tek bir defa transfekte edilmelerine göre daha yüksek oranda ve daha uzun süreli YFP ekspresyonu sağladığını göstermiştir.Sonuç: MKH’ların YFP ile işaretlenmesi çalışmalarında transfeksiyon kimyasallarının yeterli bir inkübasyondan sonra uzaklaştırılmasının ve transfeksiyon işleminin 48 saat arayla iki defa yapılmasının MKH’ların aktarıldığı doku veya canlılarda daha uzun süreli ve daha etkin bir şekilde takibine olanak sağlayacağı gösterilmiştir
Anahtar Kelime: Transfeksiyon Yeşil floresan protein Mezenkimal kök hücre Tavşanlar Modeller, hayvan

Konular: Hematoloji

Mezankimal kök hücrelerin in vivo takibinde yeşil floresan protein aktarılmasının optimizasyonu

Öz:
Objective: Green Fluorescent Protein (GFP) has been used as a marker of gene expression and a single cell marker in living organisms in cell biology studies. The important areas that GFP is used are expression levels of different genes in different organisms by inserting GFP in these genes and as a marker in living cells. In this study, we tried to optimize transfection of mesenchymal stem cells, (MSCs) used for regeneration of damaged tissues in animals, by GFP containing plasmid vector by which MSCs can be followed in vivo.Material and Methods: To this aim, phM-GFP plasmid vector carrying GFP gene and effectene transfection reagent were used.Result: The data revealed that twice transfection of MSCs resulted in higher expression of GFP for longer times as compared to once transfected MSCs. On the other hand, leaving the chemical transfection agents in the medium induced apoptosis after a while.Conclusion: As a conclusion we suggest the transfection of MSCs twice with 48 hours interval and removal of transfection agents after 8 hours which removed toxic and apoptotic effects of the chemicals.
Anahtar Kelime: Models, Animal Transfection Green Fluorescent Proteins Mesenchymal Stem Cells Rabbits

Konular: Hematoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Baran Y, URAL A, AVCU F, Sarper M, ELÇİ P, PEKEL A (2008). Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. , 172 - 175.
Chicago Baran Yusuf,URAL Ali Uğur,AVCU Ferit,Sarper Meral,ELÇİ Pınar,PEKEL Aysel Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. (2008): 172 - 175.
MLA Baran Yusuf,URAL Ali Uğur,AVCU Ferit,Sarper Meral,ELÇİ Pınar,PEKEL Aysel Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. , 2008, ss.172 - 175.
AMA Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. . 2008; 172 - 175.
Vancouver Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. . 2008; 172 - 175.
IEEE Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A "Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo." , ss.172 - 175, 2008.
ISNAD Baran, Yusuf vd. "Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo". (2008), 172-175.
APA Baran Y, URAL A, AVCU F, Sarper M, ELÇİ P, PEKEL A (2008). Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology, 25(4), 172 - 175.
Chicago Baran Yusuf,URAL Ali Uğur,AVCU Ferit,Sarper Meral,ELÇİ Pınar,PEKEL Aysel Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology 25, no.4 (2008): 172 - 175.
MLA Baran Yusuf,URAL Ali Uğur,AVCU Ferit,Sarper Meral,ELÇİ Pınar,PEKEL Aysel Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology, vol.25, no.4, 2008, ss.172 - 175.
AMA Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology. 2008; 25(4): 172 - 175.
Vancouver Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology. 2008; 25(4): 172 - 175.
IEEE Baran Y,URAL A,AVCU F,Sarper M,ELÇİ P,PEKEL A "Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo." Turkish Journal of Hematology, 25, ss.172 - 175, 2008.
ISNAD Baran, Yusuf vd. "Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo". Turkish Journal of Hematology 25/4 (2008), 172-175.