The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method

Yarim, Gul Fatma; NİSBET, Cevat; Guler, Ahmet; ÇİFTÇİ, Gülay

The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method

Cevat NİSBET, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

AHMET GÜLER, (Ondokuz Mayıs Üniversitesi, Samsun, Türkiye)

Gülay ÇİFTÇİ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Gul Fatma Yarim (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 1

Yıl: 2009 Cilt: 15 Sayı: 3 Sayfa Aralığı: 443 - 446 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method

Öz:

Balın protein içeriği ve kalitesi bitki kaynağı, balın üretim ve işleme şartlarına bağlı olarak değişir. Balların botanik kaynağını ve kalitesini belirlemek üzere birçok yöntem geliştirilmiştir. Bu çalışmanın amacını farklı bitki kaynakları (ormangülü, kestane, çiçek, çam) ve farklı besleme koşullarına (glukozlu) tabi tutulan arı kolonilerinden üretilen balların protein profilinin SDS-PAGE yöntemi ile karşılaştırmalı olarak değerlendirilmesi oluşturmaktadır. Çalışmada ormangülü, kestane, glukozlu, çiçek ve çam bitki kaynaklarından üretilen saf ballar ile yoğun şekerli (glukozlu) balın protein profilinin aynı olduğu, ancak protein yoğunluğunun farklılık gösterdiği belirlendi. Balların elektroforez analizi sonucunda 94 kDa, 87 kDa ve 84 kDa olmak üzere başlıca 3 protein bantı belirlenmiştir. Protein (%) oranları ise sırasıyla 1.1±0.07, 1.02±0.04, 0.90±0.03, 1.16±0.09 ve 0.23±0.01 olarak bulunmuştur. Ormangülü, Kestane, çiçek ve çam ballarının protein bant yoğunluklarının aşırı glukoz şerbetiyle yapılan besleme sonucu üretilen baldan yaklaşık 4-7 kat daha fazla olduğu saptandı. Bu çalışma ile saf ve hileli balların ayrımında protein profilinin değerlendirilmesinin önemli kaynak teşkil edeceği ve bu hususta yapılacak olan analizlere de destek olacağı kanısındayız.

Anahtar Kelime: SDS-PAGE bal elektroforez protein içeriği sakaroz sakkaroz şeker gıda koruyucuları

Konular: Veterinerlik

Farklı bitki türlerinden üretilmiş saf ve şekerli balların protein profilinin SDS-PAGE yöntemi ile incelenmesi

Öz:

Protein content and quality of honeys are varied according to the plant source as well as production and handling conditions. Lots of methods were developed to determine the botanical origins and qualities of honeys. The aim of this study was to comparatively evaluate the protein profiles of the honeys produced from bee colonies exposed to different plant sources (rhododendron, chestnut, blossom, pine) and feeding (Saccharose nutrition) conditions by using SDS-PAGE method. In this study, the protein profiles, but not protein densities, were found similar between pure honeys produced from rhododendron, chestnut, blossom, pine and high sugared (saccharose) honeys. Three main protein bands (94 kDa, 87 kDa and 84 kDa) were determined in the electrophoretic analysis of the honeys. Protein ratios (%) were determined as 1.31±0.07, 1.02±0.04, 0.90±0.03, 1.16±0.09 and 0.23±0.01, respectively. Protein band densities were found higher in rhododendron, chestnut and pine honeys than those in honeys produced by using excessive saccharose syrup. It was concluded that protein profile can be used in differentiation between pure and adulterated honeys.

Anahtar Kelime: sucrose saccharose sugar food preservatives SDS-PAGE honey electrophoresis protein content

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NİSBET C, Guler A, ÇİFTÇİ G, Yarim G (2009). The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. , 443 - 446.
Chicago	NİSBET Cevat,Guler Ahmet,ÇİFTÇİ Gülay,Yarim Gul Fatma The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. (2009): 443 - 446.
MLA	NİSBET Cevat,Guler Ahmet,ÇİFTÇİ Gülay,Yarim Gul Fatma The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. , 2009, ss.443 - 446.
AMA	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. . 2009; 443 - 446.
Vancouver	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. . 2009; 443 - 446.
IEEE	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G "The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method." , ss.443 - 446, 2009.
ISNAD	NİSBET, Cevat vd. "The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method". (2009), 443-446.

APA	NİSBET C, Guler A, ÇİFTÇİ G, Yarim G (2009). The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15(3), 443 - 446.
Chicago	NİSBET Cevat,Guler Ahmet,ÇİFTÇİ Gülay,Yarim Gul Fatma The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15, no.3 (2009): 443 - 446.
MLA	NİSBET Cevat,Guler Ahmet,ÇİFTÇİ Gülay,Yarim Gul Fatma The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.15, no.3, 2009, ss.443 - 446.
AMA	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(3): 443 - 446.
Vancouver	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(3): 443 - 446.
IEEE	NİSBET C,Guler A,ÇİFTÇİ G,Yarim G "The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15, ss.443 - 446, 2009.
ISNAD	NİSBET, Cevat vd. "The ınvestigation of protein prophile of different botanic origin honey and density saccharose-adulterated honey by SDS-PAGE method". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15/3 (2009), 443-446.