Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey

SEYREK, Kamil; kiral, funda; ATASOY, Abidin; KARAGENÇ, Tülin; PAŞA, Serdar

Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey

Serdar PAŞA, (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Aydın, Türkiye)

Funda KIRAL, (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Aydın, Türkiye)

Tülin KARAGENÇ, (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Aydın, Türkiye)

Abidin ATASOY, (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Aydın, Türkiye)

Kamil SEYREK (Adnan Menderes Üniversitesi, Veteriner Fakültesi, Aydın, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

6 0

Yıl: 2009 Cilt: 33 Sayı: 4 Sayfa Aralığı: 289 - 295 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey

Öz:

Bu çalışmada, Türkiye’de Ege bölgesinde Hepatozoon canis ile doğal enfekte olan 10 köpekte kaydedilen klinik ve laboratuvar bulguların tanımlanması amaçlanmıştır. H. canis enfeksiyonunun tanısı Giemsa ile boyanmış kan frotilerinin mikroskobik bakısında lökositler içerisinde gamontların görülmesi ile konuldu. Hazırlanan kan frotilerde bir alanda 500 nötrofil sayılarak H. canis’in parazitemi düzeyi belirlendi. H. canis ile enfekte köpeklerin parazitemi düzeyi % 1 ile % 23 arasında değişkenlik gösterdi. H. canis ile enfekte köpeklerde en önemli klinik bulgu olarak anoreksi, ateş, depresyon, kilo kaybı ve lenfadenopati belirlendi. Hematolojik muayenede bütün köpeklerde mikrositik hipokromik anemi belirlenirken, 10 köpeğin 7’sinde nötrofili, 1’inde lenfopeni, 2’sinde monositozis, 1’inde eozinofili, 9’unda ise trombositopeni tespit edildi. Biyokimyasal muayenede ise 10 köpeğin 7’sinde hiperproteinemi, 9’unda hipoalbuminemi, 7’sinde hiperglobulinemi, 2’sinde serum alkalen fosfataz aktivitesinde artış, 8’inde ise serum kreatin kinaz aktivitesinde artış belirlendi. H. canis ile enfekte 8 köpekte Ehrlichia canis, Anaplasma phagocytophilum ve Anaplasma platys gibi konkurent enfeksiyonların tanısı polimerize zincir reaksiyon testiyle konuldu. H. canis ile enfekte 8 köpekte konkurent enfeksiyonlardan 2 veya 3’ ü belirlendi.

Anahtar Kelime: epidemiyoloji eozinofili karışık enfeksiyon klinik görünüm Ege Bölgesi klinik muayene hastalık tetkiki hastalık yaygınlığı Türkiye Hepatozoon canis olgu sunumları trombositopeni nötrofil

Konular: Ziraat Mühendisliği

Türkiye'de Ege bölgesinde Hepatozoon canis ile doğal enfekte köpeklerin tanımlanması

Öz:

Clinical and laboratory findings recorded in 10 dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey were reported. The diagnosis was made by finding H. canis gamonts within leucocytes in Giemsastained blood smears. H. canis parasitaemia level was calculated manually by counting 500 neutrophils in blood smears. Parasitaemia varied from 1% to 23% of the circulating neutrophils. Anorexia, fever, depression, weight loss, and lymphadenopathy are the main clinical signs in infected dogs. All dogs had microcytic normochromic anaemia; however, 7 of the dogs had neutrophilia, 1 had lymphopenia, 2 had monocytosis, 1 had eosinophilia, and 9 had thrombocytopenia. Abnormal serum biochemical values were hyperproteinaemia (7 of 10 dogs), hypoalbuminaemia (9 of 10 dogs), hyperglobulinaemia (7 of 10 dogs), increased serum alkaline phosphatase activity (2 of 10 dogs), and increased serum creatinine kinase activity (8 of 10 dogs). Concurrent infections such as Ehrlichia canis, Anaplasma phagocytophilum, and Anaplasma platys were detected with a polymerase chain reaction test and 8 dogs had 2 or 3 of these concurrent infections.

Anahtar Kelime: disease surveys disease prevalence Turkey Hepatozoon canis case reports thrombocytopenia neutrophils epidemiology eosinophilia mixed infections clinical aspects Aegean Region clinical examination

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. O'Dwyer, L.H., Massard, C.L., Pereira de Souza, J.C.: Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil. Vet. Parasitol., 2001; 94: 143-150.
2. Bentley, C.A.: Preliminary note upon a Leukocytozoon of the dog. Br. Med. J., 1905; 1: 988.
3. McCully, R.M., Basson, P.A., Bigalke, R.D., De Vos, V., Young, E.: Observations on naturally acquired hepatozoonosis of wild carnivores and dogs in the Republic of South Africa. Onderstepoort J. Vet. Res., 1975; 42: 117-133.
4. Novilla, M.N., Kwapein, R.P., Peneyra, R.S.: Occurrence of canine hepatozoonosis in the Philippines. Proc. Helminthol. Soc. Washington, 1977; 44: 98-101.
5. Rajamanickam, C., Weisenhutter, E., Zin, F.M., Hamid, J.: The incidence of canine haematozoa in peninsular Malaysia. Vet. Parasitol., 1985; 17: 151-157.
6. Murata, T., Shiramizu, K., Hara, Y., Inoue, M., Shimoda, K., Nakama, S.: First case of Hepatozoon canis infection of a dog in Japan. J. Vet. Med. Sci., 1991; 53: 1097-1099.
7. Baneth, G., Weigler, B.: Retrospective case-control study of hepatozoonosis in dogs in Israel. J. Vet. Intern. Med., 1997; 11: 365-370.
8. Ezeokoli, C.D., Ogunkoya, A.B., Abdullahi, R., Tekdek, L.B., Sannusi, A., Ilemobade, A.A.: Clinical and epidemiological studies on canine hepatozoonosis in Zaria, Nigeria. J. Small Anim. Pract., 1983; 24: 455-460.
9. Baneth, G., Shkap, V., Presentey, B.Z., Pipano, E.: Hepatozoon canis: the prevalence of antibodies and gametocytes in dogs in Israel. Vet. Res. Commun., 1996; 20: 41-46.
10. Inokuma, H., Ohno, K., Yamamoto, S.: Serosurvey of Ehrlichia canis and Hepatozoon canis infection in dogs in Yamaguchi prefecture Japan. J. Vet. Med. Sci., 1999; 61; 1153-1155.
11. Karagenc, T.I., Pasa, S., Kirli, G., Hosgor, M., Bilgic, H.B., Ozon, Y.H., Atasoy, A., Eren, H.: A parasitological, molecular and serological survey of Hepatozoon canis infection in dogs around the Aegean coast of Turkey. Vet. Parasitol., 2006; 135: 113-119.
12. Tuzdil, A.N.: Bizde ilk defa görülen bir Hepatozoon canis vak’ası. Türk Baytarlar Cemiyeti Mecmuası., 1933; 13: 35.
13. Voyvoda, H., Pasa, S., Uner A.: Clinical Hepatozoon canis infection in a dog in Turkey. J. Small Anim. Pract., 2004; 45: 613-617.
14. Gavazza, A., Bizzeti, M., Papini, R.: Observations on dogs found naturally infected with Hepatozoon canis in Italy. Rev. Méd. Vét., 2003; 154: 565-571.
15. Forlano, M., Scofield, A., Elisei, C., Fernandes, K.R., Ewing, S.A., Massard, C.L.: Diagnosis of Hepatozoon spp. in Amblyomma ovale and its experimental transmission in domestic dogs in Brazil. Vet. Parasitol., 2005; 134: 1-7.
16. Baneth, G., Samish, M., Shkap, V.: Life cycle of Hepatozoon canis (Apicomplexa: Adelorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). J. Parasitol., 2007; 93: 283-299.
17. Elias, E., Homans, P.A.: Hepatozoon canis infection in dogs: clinical and hematological findings; treatment. J. Small Anim. Pract., 1988; 29: 55-62.
18. Inokuma, H., Okuda, M., Ohno, K., Shimoda, K., Onishi, T.: Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Vet. Parasitol., 2002; 106: 265- 271.
19. Jain, N.C.: Schalm’s Veterinary Hematology. 4th edn., Lea and Febriger, Philadelphia, 1986.
20. Oura, C.A.L, Bishop, R.P., Wampande, E.M., Lubega, G.W., Tait A.: Application of a reverse line blot assay to the study of haemoparasites in cattle in Uganda. Int. J. Parasitol., 2004; 34: 603-613.
21. Murphy, G.L., Ewing, S.A., Whitworth, L.C., Fox, J.C., Kocan, A.A.: A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet. Parasitol., 1998; 79: 325-339.
22. Inokuma, H., Fujii, K., Matsumoto, K., Okuda, M., Nakagome, K., Kosugi, R., Hirakawa, M., Onishi, T.: Demonstration of Anaplasma (Ehrlichia) platys inclusions in peripheral blood platelets of a dog in Japan. Vet. Parasitol., 2002; 110: 145-152.
23. Bekker, C.P., de Vos, S., Taoufik, A., Sparagano, O.A., Jongejan F.: Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatumticks by reverse line blot hybridization. Vet Microbiol., 2002; 89: 223-238.
24. Duncan, R.J., Prasse, W.K.: Veterinary Laboratory Medicine. 2nd edn. Iowa State University Press, Ames, Iowa. 1986; 227- 234.
25. Morgan, R.V.: Handbook of Small Animal Practice. Churchill Livingstone, New York, 1992; 1378-1384.
26. Baneth, G., Samish, M., Alekseev, E., Aroch, I., Shkap, V.: Transmission of Hepatozoon canis to dogs by naturally-fed or percutaneously-injected Rhipicephalus sanguineus ticks. J. Parasitol., 2001; 87: 606-611.
27. Shaw, S.E., Day, M.J., Birtles, R.J., Breitschwerdt, E.B.: Tickborne infectious diseases of dogs. Trends Parasitol., 2001; 17: 74-80.
28. Mylonakis, M.E., Koutinas, A.F., Baneth, G., Polizopoulou, Z., Fytianou, A.: Mixed Ehrlichia canis, Hepatozoon canis, and presumptive Anaplasma phagocytophilum infection in a dog. Vet. Clin. Pathol., 2004; 33: 249-251.
29. Kuehn, N.F., Gaunt, S.D.: Clinical and hematological findings in canine ehrlichiosis. J. Am. Vet. Med. Assoc., 1985; 186: 355- 358.
30. Gondim, L.F.P., Kohayagawa, A., Alencar, N.X., Biondo, A.W., Takahira, R.K., Franco, S.R.: Canine hepatozoonosis in Brazil: description of eight naturally occurring cases. Vet. Parasitol., 1998; 74: 319- 323.
31. O'Dwyer, L.H., Saito, M.E., Hasegawa, M.Y., Kohayagawa, A.: Prevalence, hematology and serum biochemistry in stray dogs naturally infected by Hepatozoon canis in Sâo Paulo. Arq. Bras. Med. Vet. Zootec., 2006; 58: 688-690.

APA	PAŞA S, kiral f, KARAGENÇ T, ATASOY A, SEYREK K (2009). Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. , 289 - 295.
Chicago	PAŞA Serdar,kiral funda,KARAGENÇ Tülin,ATASOY Abidin,SEYREK Kamil Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. (2009): 289 - 295.
MLA	PAŞA Serdar,kiral funda,KARAGENÇ Tülin,ATASOY Abidin,SEYREK Kamil Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. , 2009, ss.289 - 295.
AMA	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. . 2009; 289 - 295.
Vancouver	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. . 2009; 289 - 295.
IEEE	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K "Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey." , ss.289 - 295, 2009.
ISNAD	PAŞA, Serdar vd. "Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey". (2009), 289-295.

APA	PAŞA S, kiral f, KARAGENÇ T, ATASOY A, SEYREK K (2009). Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turkish Journal of Veterinary and Animal Sciences, 33(4), 289 - 295.
Chicago	PAŞA Serdar,kiral funda,KARAGENÇ Tülin,ATASOY Abidin,SEYREK Kamil Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turkish Journal of Veterinary and Animal Sciences 33, no.4 (2009): 289 - 295.
MLA	PAŞA Serdar,kiral funda,KARAGENÇ Tülin,ATASOY Abidin,SEYREK Kamil Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.33, no.4, 2009, ss.289 - 295.
AMA	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(4): 289 - 295.
Vancouver	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(4): 289 - 295.
IEEE	PAŞA S,kiral f,KARAGENÇ T,ATASOY A,SEYREK K "Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey." Turkish Journal of Veterinary and Animal Sciences, 33, ss.289 - 295, 2009.
ISNAD	PAŞA, Serdar vd. "Description of dogs naturally infected with Hepatozoon canis in the Aegean region of Turkey". Turkish Journal of Veterinary and Animal Sciences 33/4 (2009), 289-295.