Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

KAHYA DEMİRBİLEK, SERPİL; ŞEN, Ayşin; BÜYÜKCANGAZ, ESRA

Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Esra BÜYÜKCANGAZ, (Uludağ Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Bursa)

Ayşin ŞEN, (Uludağ Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Bursa)

SERPİL KAHYA DEMİRBİLEK (Uludağ Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Bursa)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2009 Cilt: 33 Sayı: 4 Sayfa Aralığı: 311 - 316 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Öz:

Kuzeybatı Türkiye’de 2004-2005 yılları arasındaki iki kuzulama döneminde 55 adet koyun ve 10 adet keçi olmak üzere toplam 65 atık vakası Brucella spp. yönünden incelendi. İzolatların tür ve biyotip identifikasyonları için koloni morfolojisi, akriflavin ile aglütinasyon, H2S üretimi, CO2 gereksinimi, tiyonin ve basik fuksin varlığında üreme, Tbilisi fajı ile lizis, streptomisinli besi yerinde üreme, monospesifik A ve M antiserumlarla aglütinasyon özellikleri incelendi. Çalışmada kullanılan 55 koyun atığının 21 adedi, 10 keçi atığının 1 adedi Brucella melitensis olarak identifiye edildi. Biyotiplendirme sonucunda, 22 izolatın 14, 6 ve 1 adedinin sırasıyla Brucella melitensis biyotip 3, B. melitensis biyotip 1 B. melitensis biyotip 2 olduğu ortaya kondu. Koyun orijinli bir adet izolatın ise H2S üretimi yönünden pozitif olduğu saptandı ve atipik B.melitensis olarak değerlendirildi.

Anahtar Kelime: epidemiyoloji keçi fetal ölüm Brucella melitensis hastalık tetkiki koyun hastalık yaygınlığı yavru atma soy izolasyon

Konular: Ziraat Mühendisliği

Atık koyun keçi fetuslarından Brucella melitensis'in izolasyonu ve biyotiplendirilmesi

Öz:

The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. melitensis biotype 3, B. melitensis biotype 1, and B. melitensis biotype 2, respectively. One strain isolated from a sheep abortion case was H2S- producing and was identified as atypical B. melitensis.

Anahtar Kelime: Brucella melitensis disease surveys sheep disease prevalence abortion strains isolation epidemiology goats fetal death

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BÜYÜKCANGAZ E, ŞEN A, KAHYA DEMİRBİLEK S (2009). Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. , 311 - 316.
Chicago	BÜYÜKCANGAZ ESRA,ŞEN Ayşin,KAHYA DEMİRBİLEK SERPİL Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. (2009): 311 - 316.
MLA	BÜYÜKCANGAZ ESRA,ŞEN Ayşin,KAHYA DEMİRBİLEK SERPİL Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. , 2009, ss.311 - 316.
AMA	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. . 2009; 311 - 316.
Vancouver	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. . 2009; 311 - 316.
IEEE	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S "Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses." , ss.311 - 316, 2009.
ISNAD	BÜYÜKCANGAZ, ESRA vd. "Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses". (2009), 311-316.

APA	BÜYÜKCANGAZ E, ŞEN A, KAHYA DEMİRBİLEK S (2009). Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turkish Journal of Veterinary and Animal Sciences, 33(4), 311 - 316.
Chicago	BÜYÜKCANGAZ ESRA,ŞEN Ayşin,KAHYA DEMİRBİLEK SERPİL Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turkish Journal of Veterinary and Animal Sciences 33, no.4 (2009): 311 - 316.
MLA	BÜYÜKCANGAZ ESRA,ŞEN Ayşin,KAHYA DEMİRBİLEK SERPİL Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turkish Journal of Veterinary and Animal Sciences, vol.33, no.4, 2009, ss.311 - 316.
AMA	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(4): 311 - 316.
Vancouver	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turkish Journal of Veterinary and Animal Sciences. 2009; 33(4): 311 - 316.
IEEE	BÜYÜKCANGAZ E,ŞEN A,KAHYA DEMİRBİLEK S "Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses." Turkish Journal of Veterinary and Animal Sciences, 33, ss.311 - 316, 2009.
ISNAD	BÜYÜKCANGAZ, ESRA vd. "Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses". Turkish Journal of Veterinary and Animal Sciences 33/4 (2009), 311-316.