Yıl: 2009 Cilt: 15 Sayı: 5 Sayfa Aralığı: 661 - 668 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats

Öz:
Bu çalışma, kültür mediumu ve serumun in vitro rat spermleri üzerine etkisini incelemek amacıyla planlanmıştır. %10’luk fetal bovine serum (FBS) içeren veya içermeyen çeşitli kültür mediumlarının spermatid ve spermler üzerine etkileri Wistar tipi ratlar üzerinde in vitro olarak değerlendirildi. Spermatozoonlar Gamete-20, RPMI-1640, alphaMEM (AMEM), Dulbecco's modified Eagle's medium (DMEM) ve Eagle's MEM (MEM)’de inkübe edildi/kültür yapıldı. Hücre sayısı ve morfolojisi 4. ve 24. saatlerde kaydedildi. Tüm koşullarda zamanla hücre sayısı ve canlılığında azalma tesbit edildi. Parametrelerin FBS varlığında olumlu yönde değiştiği görüldü. Tüm spermatid ve spermatozoonların yaşam oranları 24 saatlik kültürden sonra azalırken; en iyi hücre morfolojisi ve yaşam oranları serumlu RPMI, en kötü hücre morfolojisi ve yaşam oranları ise serumsuz MEM’de tesbit edildi. Sonuç olarak kültür mediumunun bileşenlerinin ve FBS’nin, spermatozoonların olgunlaşması ve yaşaması için gerekli olduğu; uygun seçilmiş kültür ortamının, fonksiyonel sperm oluşturulmasının yanı sıra in vitro fertilizasyon (IVF) uygulamalarının başarısında da önemli rol oynayabileceği düşünülmektedir.
Anahtar Kelime: yaşayabilme fetal sığır serumu spermatozoa in vitro olgunlaşma sperm kültür ortamı in vitro döllenme sıçan serum spermatidler

Konular: Veterinerlik

Erkek wistar ratlarda kültür medyumunun ve serumun testis ve epididimis germinal hücreleri üzerine in-vitro etkileri

Öz:
The present study was designed to examine the influence of culture media and serum on survival of in vitro rat sperms. The effects of different culture media with or without 10% fetal bovine serum (FBS) on spermatids and spermiums of Wistar rats were assessed in vitro between 2004 and 2005. Spermatozoa were cultured in Gamete-20, RPMI-1640, alphaMEM (AMEM), Dulbecco's modified Eagle's medium (DMEM) and Eagle's MEM (MEM). The number and morphology of cells was recorded at 4 and 24 h. Under all conditions the number and the viability of the cells decreased with time but parameters were positively affected by the presence of FBS. Viability rates of spermatids and spermatozoa reduced at 24 h of culturing. The survival and morphology were the best in RPMI with serum and worst in MEM without serum. These results indicate that different composition of culture media and FBS are important for maturation and survival of spermatozoa. Carefully selected culture media can play important roles in the generation of functional sperms as well as in the success of in-vitro fertilization (IVF) procedures.
Anahtar Kelime: in vitro fertilization rats serum spermatids survival fetal bovine serum spermatozoa in vitro maturation sperm culture media

Konular: Veterinerlik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA CEZAYİRLİ E, TUĞLU M, vural k (2009). In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. , 661 - 668.
Chicago CEZAYİRLİ Enis,TUĞLU M. İbrahim,vural kamil In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. (2009): 661 - 668.
MLA CEZAYİRLİ Enis,TUĞLU M. İbrahim,vural kamil In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. , 2009, ss.661 - 668.
AMA CEZAYİRLİ E,TUĞLU M,vural k In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. . 2009; 661 - 668.
Vancouver CEZAYİRLİ E,TUĞLU M,vural k In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. . 2009; 661 - 668.
IEEE CEZAYİRLİ E,TUĞLU M,vural k "In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats." , ss.661 - 668, 2009.
ISNAD CEZAYİRLİ, Enis vd. "In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats". (2009), 661-668.
APA CEZAYİRLİ E, TUĞLU M, vural k (2009). In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15(5), 661 - 668.
Chicago CEZAYİRLİ Enis,TUĞLU M. İbrahim,vural kamil In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15, no.5 (2009): 661 - 668.
MLA CEZAYİRLİ Enis,TUĞLU M. İbrahim,vural kamil In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.15, no.5, 2009, ss.661 - 668.
AMA CEZAYİRLİ E,TUĞLU M,vural k In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(5): 661 - 668.
Vancouver CEZAYİRLİ E,TUĞLU M,vural k In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2009; 15(5): 661 - 668.
IEEE CEZAYİRLİ E,TUĞLU M,vural k "In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15, ss.661 - 668, 2009.
ISNAD CEZAYİRLİ, Enis vd. "In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 15/5 (2009), 661-668.