Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic

EMEK, Yelda Çalmaz; ERDAĞ, Bengi Baba

Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic

Bengi Baba ERDAĞ, (Adnan Menderes Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Aydın, Türkiye)

Yelda Çalmaz EMEK (Adnan Menderes Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Aydın, Türkiye)

Turkish Journal of Biology

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Yıl: 2009 Cilt: 33 Sayı: 4 Sayfa Aralığı: 319 - 326 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic

Öz:

Kritik olarak tehdit altındaki Türkiye endemiği Anthemis xylopoda O.Schwarz’ın in vitro üretilmiş sürgünlerinin yaprak eksplantlarından doğrudan adventif sürgün oluşumu için bir yöntem geliştirilmiştir. Rejenerasyon denemelerinde $N^6$+–benzyladenine (BA), kinetin (KIN) ve thidiazuron (TDZ) ilaveli Murashige ve Skoog (MS) besi ortamları kullanılmıştır. 0,5 mg $L^{-1}$ BA konsantrasyonu iyi kalitedeki adventif sürgünlerin en yüksek sayısını vermesi nedeni ile optimum uygulama olarak seçilmiştir (6,70 ± 1,05 sürgün/eksplant). En yüksek maksimum sürgün boyu ortalaması 0,2 mg $L^{-1}$ BA içeren MS ortamında gözlenmiştir. Rejenere olmuş sürgünler en iyi 0,5 mg $L^{-1}$ indol-3-butirik asit (IBA) ilaveli ½ MS ortamında köklenmişlerdir. Köklenme sırasında çiçek tomurcukları da oluşmuştur. Bu çalışmada, in vitro çiçek tomurcuklarının oluşumu için ortamda oksinlerin varlığı gereklidir. A. xylopoda’nın in vitro çiçek tomurcuklarının oluşumunda IBA, indol-3-asetik asit ’den (IAA) daha verimlidir. Adventif sürgünler 1,0 mg $L^{-1}$ IBA içeren ½MS ortamına kültüre edildiklerinde en yüksek çiçeklenme yüzdesi elde edilmiştir. In vitro üretilmiş çiçekler morfolojik olarak normal görünümlü ve sarı renklidir.

Anahtar Kelime: Anthemis Anthemis xylopoda Asteraceae yapay gençleştirme adventif sürgün endemik bitkiler Türkiye in vitro kültür çiçeklenme in vitro yenilenme

Konular: Biyoloji

Kritik olarak tehdit altındaki Türkiye endemiği Anthemis xylopoda O.Schwarz’nın adventif sürgün rejenerasyonu ve in vitro çiçeklenmesi

Öz:

A protocol has been developed for the direct induction of adventitious shoots from leaf explants of in vitro raised Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. The Murashige and Skoog (MS) media supplemented with $N^6$–benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) were used in regeneration experiments. BA at 0.5 mg $L^{-1}$ concentration was chosen as the optimal treatment because it yielded the maximal number of adventitious shoots with the best quality (6.70 ± 1.05 shoots/explant). The highest mean of maximum shoot length was observed in MS media containing 0.2 mg $L^{-1}$ BA (4.30 ± 0.48 cm). Regenerated shoots rooted best on ½MS medium containing 0.5 mg $L^{-1}$ indole-3-butyric acid (IBA). Flower buds also occurred during rooting. For this study, the existence of auxins in the medium was essential for in vitro flower bud induction. IBA was found to be more efficient than indole- 3-acetic acid (IAA) in the induction of flower buds of A. xylopoda. The maximum flowering percentage was obtained when the shoots were cultured on ½MS medium containing 1.0 mg $L^{-1}$ IBA. Flowers produced in vitro were morphologically normal and yellow.

Anahtar Kelime: adventitious shoots endemic plants Turkey in vitro culture flowering in vitro regeneration Anthemis Anthemis xylopoda Asteraceae artificial regeneration

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERDAĞ B, EMEK Y (2009). Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. , 319 - 326.
Chicago	ERDAĞ Bengi Baba,EMEK Yelda Çalmaz Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. (2009): 319 - 326.
MLA	ERDAĞ Bengi Baba,EMEK Yelda Çalmaz Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. , 2009, ss.319 - 326.
AMA	ERDAĞ B,EMEK Y Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. . 2009; 319 - 326.
Vancouver	ERDAĞ B,EMEK Y Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. . 2009; 319 - 326.
IEEE	ERDAĞ B,EMEK Y "Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic." , ss.319 - 326, 2009.
ISNAD	ERDAĞ, Bengi Baba - EMEK, Yelda Çalmaz. "Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic". (2009), 319-326.

APA	ERDAĞ B, EMEK Y (2009). Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. Turkish Journal of Biology, 33(4), 319 - 326.
Chicago	ERDAĞ Bengi Baba,EMEK Yelda Çalmaz Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. Turkish Journal of Biology 33, no.4 (2009): 319 - 326.
MLA	ERDAĞ Bengi Baba,EMEK Yelda Çalmaz Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. Turkish Journal of Biology, vol.33, no.4, 2009, ss.319 - 326.
AMA	ERDAĞ B,EMEK Y Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. Turkish Journal of Biology. 2009; 33(4): 319 - 326.
Vancouver	ERDAĞ B,EMEK Y Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. Turkish Journal of Biology. 2009; 33(4): 319 - 326.
IEEE	ERDAĞ B,EMEK Y "Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic." Turkish Journal of Biology, 33, ss.319 - 326, 2009.
ISNAD	ERDAĞ, Bengi Baba - EMEK, Yelda Çalmaz. "Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic". Turkish Journal of Biology 33/4 (2009), 319-326.