Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques

KARGIOGLU, MUSTAFA; DAYAN, SERGUN; Temel, Mehmet; CENKCİ, Süleyman

Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques

Süleyman CENKCİ, (Afyon Kocatepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Afyon, Türkiye)

Mehmet TEMEL, (Afyon Kocatepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Afyon, Türkiye)

Mustafa KARGIOGLU, (Afyon Kocatepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Afyon, Türkiye)

Sergun DAYAN (Afyon Kocatepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Afyon, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2009 Cilt: 33 Sayı: 4 Sayfa Aralığı: 327 - 333 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques

Öz:

Bu rapor, tehlike altındaki T. turcica’nın rizom çelikleri ve epikotil eksplantları kullanılarak klonal çoğaltmasını kapsamaktadır. Rizom çelikler dikilmeden önce vejetatif çoğaltma için α-naftalenasetik asit (NAA) veya indol-3-butrik asit (IBA) ile ön muameleye tutulmuştur. NAA (10 mg/L) ile ön muamele edilmiş rizom çelikler 100 gün sonunda hem köklenmiş hem de filizlenmiştir (% 66,6). Epikotil eksplanlarında, NAA uygulaması kallus ve adventif kök oluşumunu ve 6-benziladenin (BA) uygulaması mikro-fide üretimini teşvik etmiştir. BA ile birlikte düşük NAA değerleri (0,5-1 μM) fidelenmeye uyarımı ve fide gelişimini arttırmıştır. En yüksek rejenerasyon oranı (% 86,6), explant başına ortalama fide sayısı (3,05) ve ortalama fide uzunluğu (2,3 cm) 10 μM BA ve 0,5 μM NAA ile başarılmıştır. In vitro rejenere fidelerin yaklaşık % 83’ü 0,3 μM NAA ile desteklenmiş ½ Murashige ve Skoog (MS) besininde köklenmiştir. In vitro bitkicikler morfolojik olarak normal ve kök uçlarındaki sabit kromozom sayısı 2n = 18 olarak tespit edilmiştir. Çalışma, hem geleneksel hem de in vitro tekniklerin tehlike altındaki bu bitki türünün seri üretilmesinde ve çoğaltılmasında kullanışlı olabileceğini göstermiştir.

Anahtar Kelime: soyu tükenmekte olan türler eksplant rizom çelikler NAA epikotil Fabaceae benziladenin çoğaltma Thermopsis turcica in vitro kültür koruma

Konular: Biyoloji

Tehlike altındaki Thermopsis turcica’nın Kit Tan, Vural & Küçüködük geleneksel ve in vitro teknikler kullanılarak çoğaltımı

Öz:

This report deals with the successful clonal propagation of endangered T. turcica using rhizome cuttings and epicotyl explants. Rhizome cuttings were treated with α-naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA) before planting for vegetative multiplication. Rhizome cuttings pretreated with NAA (10 mg/L) were both rooted and sprouted (66.6%) after 100 days. Application ofNAA induced callus and adventitious root formationin epicotyl explants and 6-benzyladenine (BA) induced production of microshoots. Low levels of NAA (0.5-1 μM) together with BApromoted shoot initiation and development. The highest regeneration rate (86.6%), with a mean number of shoots (3.05) and a mean length of shoots (2.3 cm) per epicotyl, was achieved at 10 μM BA and 0.5 μM NAA. About 83% of in vitro regenerated shoots rooted on a ½ Murashige and Skoog (MS) medium supplemented with 0.3 μM NAA. In vitro plantlets were morphologically normal and a uniform chromosome complement of 2n = 18 was detected in root tips. The study demonstrated that both conventional and in vitro techniques could be useful for large scale multiplication and propagation of this endangered plant species.

Anahtar Kelime: Fabaceae benzyladenine propagation Thermopsis turcica in vitro culture conservation endangered species explants rhizomes cuttings NAA epicotyls

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	CENKCİ S, Temel M, KARGIOGLU M, DAYAN S (2009). Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. , 327 - 333.
Chicago	CENKCİ Süleyman,Temel Mehmet,KARGIOGLU MUSTAFA,DAYAN SERGUN Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. (2009): 327 - 333.
MLA	CENKCİ Süleyman,Temel Mehmet,KARGIOGLU MUSTAFA,DAYAN SERGUN Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. , 2009, ss.327 - 333.
AMA	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. . 2009; 327 - 333.
Vancouver	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. . 2009; 327 - 333.
IEEE	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S "Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques." , ss.327 - 333, 2009.
ISNAD	CENKCİ, Süleyman vd. "Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques". (2009), 327-333.

APA	CENKCİ S, Temel M, KARGIOGLU M, DAYAN S (2009). Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. Turkish Journal of Biology, 33(4), 327 - 333.
Chicago	CENKCİ Süleyman,Temel Mehmet,KARGIOGLU MUSTAFA,DAYAN SERGUN Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. Turkish Journal of Biology 33, no.4 (2009): 327 - 333.
MLA	CENKCİ Süleyman,Temel Mehmet,KARGIOGLU MUSTAFA,DAYAN SERGUN Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. Turkish Journal of Biology, vol.33, no.4, 2009, ss.327 - 333.
AMA	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. Turkish Journal of Biology. 2009; 33(4): 327 - 333.
Vancouver	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques. Turkish Journal of Biology. 2009; 33(4): 327 - 333.
IEEE	CENKCİ S,Temel M,KARGIOGLU M,DAYAN S "Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques." Turkish Journal of Biology, 33, ss.327 - 333, 2009.
ISNAD	CENKCİ, Süleyman vd. "Propagation of endangered Thermopsis turcica Kit Tan, Vural & Küçüködük using conventional and in vitro techniques". Turkish Journal of Biology 33/4 (2009), 327-333.