Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows

SELÇUK, MURAT; CEVIK, MESUT; DOGAN, Soner

Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows

Mesut ÇEVİK, (Ondokuz Mayıs Üniversitesi, Samsun, Türkiye)

Murat SELÇUK, (Ondokuz Mayıs Üniversitesi, Samsun, Türkiye)

Soner DOGAN (Univercity of Minnesota, Hormel Research Institute, USA)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2010 Cilt: 16 Sayı: 1 Sayfa Aralığı: 85 - 89 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows

Öz:

Çalışmanın amacı, laktasyondaki sütçü ineklerin gebelik oranları üzerinde üç farklı östrus senkronizasyon protokolünün etkilerini değerlendirmektir. Grup 1’deki (Ovsynch protokolü; n=20) hayvanlara 0. günde 20 μg GnRH, 7. günde 500 μg PGF2α ve 9. günde 20 μg GnRH verildi; Grup 2’deki (Heatsynch protokolü; n=20) hayvanlara, 0. günde 20 μg GnRH, 7. günde 500 μg PGF2α ve 8. günde 1 ml estradiol cypionate i.m. yapıldı; Grup 3’teki (CIDR protokolü; n=20) hayvanlara ise 0. günde intravaginal olarak CIDR yerleştirildi, 6. günde 500 μg PGF2α ve 7. günde CIDR vaginadan uzaklaştırıldı. Uygulamaya alınan hayvanların tamamına 10. günde suni tohumlama yapıldı. Gebelik tanısı suni tohumlamadan sonraki 35±3 günlerde transrektal ultrasonografi ile yapıldı. Heatsynch grubunda östrus aktivitesi Ovsynch ve CIDR gruplarına oranla daha önemli derecede yüksek gözlendi (P<0.05). 7. ve 10. günlerde yapılan vaginal elektriksel direnç ölçümlerinde her 3 grup arasında önemli farklılıklar bulundu (P<0.05). Ovsynch, Heatsynch ve CIDR grupları arasında 1. ve 2. suni tohumlamalardan elde edilen gebelik oranları açısından farklılık bulunmadı (P>0.05). Sonuç olarak, laktasyondaki ineklerde tatmin edici gebelik oranları veren bu 3 senkronizasyon protokolü eşdeğerli olarak kullanılabilir.

Anahtar Kelime: laktasyon sentetik hormonlar kızgınlık östradiol gebelik oranları eş zamanlama suni tohumlama GnRH süt ineği

Konular: Veterinerlik

Sütçü ineklerde ovsynch, Heatsych ve CIDR senkronizasyon protokollerinde uygulanan sabit zamanlı suni tohumlama gebelik oranlarının karşılaştırılması

Öz:

The objective of this study was to compare the effects of three different estrus synchronization protocols on the pregnancy rates of lactating dairy cows. Group 1 (Ovsynch protocol; n=20) animals received 20 μg GnRH on day 0, 500 μg PGF2α on day 7 and 20 μg GnRH on day 9; Group 2 (Heatsynch protocol; n=20) received 20 μg GnRH on day 0, 500 μg PGF2α on day 7 and an injection of 1 ml estradiol cypionate i.m. on day 8; Group 3 (CIDR protocol; n=20) an intravaginal CIDR insertion on day 0, inject with 500 μg PGF2α on day 6, and removed CIDR on day 7. All animals were fixed-time artificial inseminated (FTAI) on day 10. Pregnancy diagnosis was performed on all animals via transrectal ultrasonography at 35±3 days following FTAI. Estrus activity observed in the Heatsynch group animals was higher than that in the Ovsynch and CIDR-based groups (P<0.05). There were significant differences among groups for vaginal electrical resistance in days 7 and 10 (P<0.05), but there was no significant difference among groups on first and second service pregnancy rates (P>0.05). In conclusion, these three synchronization protocols providing satisfactory pregnancy rates in lactating dairy cows can be equally usable.

Anahtar Kelime: dairy cows lactation synthetic hormones oestrus estradiol pregnancy rate synchronization artificial insemination GnRH

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1.Bartolome JA, Silvestre FT, Kamimura S, Arteche ACM, Melendez P, Kelbert D, McHale J, Swift K, Archbald LF, Thatcher WW: Resynchronization of ovulation and timed insemination in lactating dairy cows I: Use of the Ovsynch and Heatsynch protocols after non-pregnancy diagnosis by ultrasonography. Theriogenology, 63, 1617-1627, 2005.
2.Martinez MF, Adams GP, Kastelic JP, Bergfelt DR, Mapletoft RJ: Induction of follicular wave emergence for estrus synchronization and artificial insemination in heifers. Theriogenology, 54, 757-769, 2000.
3.Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA, Anderson LL: Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci, 80, 295-300, 1997.
4.Kaçar C, Kamiloğlu NN, Uçar Ö, Arı UÇ, Pancarcı ŞM, Güngör Ö: Effect of Ovsynch and Cosynch synchronisation programmes combined with beta-carotene + vitamin E administration upon the pregnancy rates in cows. Kafkas Üniv Vet Fak Derg, 14(1): 45-50, 2008.
5.Stevenson JS, Tiffany SM, Lucy MC: Use of estradiol cypionate as a substitute for GnRH in Protocols for synchronizing ovulation in dairy cattle. J Dairy Sci, 87, 32983305, 2004.
6.Cartmill JA, El-Zarkouny SZ, Hensley BA, Lamb GC, Stevenson JS: Stage of cycle, incidence, and timing of ovulation, and pregnancy rates in dairy cattle after three timed breeding protocols. J Dairy Sci, 84, 1051-1059, 2001.
7.Tenhagen B-A, Surholt R, Wittke M, Vogel C, Drillich M, Heuwieser W: Use of Ovsynch in dairy herds differences between primiparous and multiparous cows. Anim Reprod Sci, 81, 1-11, 2004.
8.Pancarci SM, Jordan ER, Risco CA, Schouten MJ, Lopes FL, Moreira F, Thatcher WW: Use of estradiol cypionate in a presynchronized timed artificial insemination program for lactating dairy cattle. J Dairy Sci, 85, 122-131, 2002.
9.Pursley JR, Kosorok MR, Wiltbank MC: Reproductive management of lactating dairy cows using synchronization of ovulation. J Dairy Sci, 80, 301-306, 1997.
10.Kasimanickam R, Cornwell JM, Nebel RL: Fertility following fixed-time AI or insemination at observed estrus in Ovsynch and Heatsynch programs in lactating dairy cows. Theriogenology, 63, 2550-2559, 2005.
11.Çelik HA, Avcı G, Aydın İ, Bülbül A, Bülbül T: Effect of beta-carotene on ovarium functions and Ovsynch success in repeat breeder cows. Kafkas Üniv Vet Fak Derg, 15, 87-94, 2009.
12.Rabiee AR, Lean IJ, Stevenson MA: Efficacy of ovsynch program on reproductive performance in dairy cattle: A Metaanalysis. J Dairy Sci, 88, 2754-2770, 2005.
13.Mapletoft RJ, Martinez MF, Colazo MG, Kastelic JP: The use of controlled internal drug release devices for the regulation of bovine reproduction. J Anim Sci, 81 (E. Suppl. 2): 28-36, 2003.
14.Colazo MG, Kastelic JP, Mapletoft RJ: Effects of estradiol cypionate (ECP) on ovarian follicular dynamics, synchrony of ovulation, and fertility in CIDR-based, fixed-time AI programs in beef heifers. Theriogenology, 60, 855-865, 2003.
15.Kim UH, Suh GH, Nam HW, Kang HG, Kim IH: Follicular wave emergence, luteal fuction and synchrony of ovulation following GnRH or estradiol benzoate in a CIDR-treated, lactating Holstein cows. Theriogenology, 63, 260-268, 2005.
16.Lucy MC, Billings HJ, Butler WR, Ehniss LR, Fields MJ, Kesler DJ, Kinder JE, Mattos RC, Short RE, Thatcher WW, Wettemann RP, Yelich JV, Hafs HD: Efficacy of an intravaginal progesterone insert and an injection of PGF2α for synchronizing estrus and shortening the interval to pregnancy in postpartum beef cows, peripubertal beef heifers, and dairy heifers. J Anim Sci, 79, 982-995, 2001.
17.Peeler ID, Nebel RL, Pearson RE: Pregnancy rates after timed AI of heifers following removal of intravaginal progesterone inserts. J Dairy Sci, 87, 2868-2873, 2004.
18.Edmonson AJ, Lean IJ, Weaver LD, Farver T, Webster G: A body condition scoring chart for Holstein dairy cows. J Dairy Sci, 72, 68-78, 1989.
19. SAS: Statistical Software Program. SAS User Guide Ver 9.01, SAS Campus Drive, Cary NC 27513 USA, 2007.
20. Alnimer M, De Rosa G, Grasso F, Napolitano F, Bordi A: Effect of climate on the response to three oestrous synchronization techniques in lactating dairy cows. Anim Reprod Sci, 71, 157-168, 2002.
21.Ahmadi MR, Nazifi S, Sajedianfard J, Moattari G: Impact of estrous synchronization methods on cellular proportions in cervical mucus and serum hormone concentrations. Theriogenology, 67, 598-604, 2007.
22.Meena RS, Sharma SS, Purohit GN: Efficiency of vaginal electrical resistance measurements for oestrous detection and insemination in Rathi cows. Br Soc Anim Sci, 76, 433-437, 2003.
23.Taşal I, Ataman MB, Aksoy M, Kaya A, Karaca F, Tekeli T: Estimation of early pregnancy by electrical resistance values of vaginal mucosa in cows and heifers. Rev Med Vet, 156, 91-94, 2005.
24.Kasimanickam R, Hall JB, Currin JF, Whittier WD: Sire effect on the pregnancy outcome in beef cows synchronized with progesterone based Ovsynch and Co-synch protocols. Anim Reprod Sci, 104, 1-8, 2008.
25.Neglia G, Gasparini B, Di Palo R, De Rosa C, Zicarelli L, Campanile G: Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranen Buffalo cows. Theriogenology, 60, 125-133, 2003.
26.Burke JM, De La Sota RL, Risco CA, Staples CR, Schmitt EJP, Thatcher WW: Evaluation of timed insemination using a gonadotropin-releasing hormone agonist in lactating dairy cows. J Dairy Sci, 79, 1385-1393, 1996.
27.Chebel RC, Santos JEP, Cerri RLA, Rutigliano HM, Bruno RGS: Reproduction in dairy cows following progesterone inserts presychronizaion and resynchronization protocols. J Dairy Sci, 89, 4205-4219, 2006.
28.Cavalieri J, Hepworth G, Fitzpatrick LA: Comparison of two estrus synchronization and resynchronization treatments in lactating dairy cows. Theriogenology, 62, 729-747, 2004.

APA	CEVIK M, SELÇUK M, DOGAN S (2010). Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. , 85 - 89.
Chicago	CEVIK MESUT,SELÇUK MURAT,DOGAN Soner Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. (2010): 85 - 89.
MLA	CEVIK MESUT,SELÇUK MURAT,DOGAN Soner Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. , 2010, ss.85 - 89.
AMA	CEVIK M,SELÇUK M,DOGAN S Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. . 2010; 85 - 89.
Vancouver	CEVIK M,SELÇUK M,DOGAN S Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. . 2010; 85 - 89.
IEEE	CEVIK M,SELÇUK M,DOGAN S "Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows." , ss.85 - 89, 2010.
ISNAD	CEVIK, MESUT vd. "Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows". (2010), 85-89.

APA	CEVIK M, SELÇUK M, DOGAN S (2010). Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(1), 85 - 89.
Chicago	CEVIK MESUT,SELÇUK MURAT,DOGAN Soner Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.1 (2010): 85 - 89.
MLA	CEVIK MESUT,SELÇUK MURAT,DOGAN Soner Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.1, 2010, ss.85 - 89.
AMA	CEVIK M,SELÇUK M,DOGAN S Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(1): 85 - 89.
Vancouver	CEVIK M,SELÇUK M,DOGAN S Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(1): 85 - 89.
IEEE	CEVIK M,SELÇUK M,DOGAN S "Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.85 - 89, 2010.
ISNAD	CEVIK, MESUT vd. "Comparison of pregnancy rates after timed artificial insemination in ovsynch, Heatsynch and CIDR-based synchronization protocol in dairy cows". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/1 (2010), 85-89.