The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice

KARABULUT, Aysun Bay; CENGİZ, ARA; ÖZGÖR, Dinçer; KAHRAMAN, Latif; YAMAN, Hilmi; Dirican, Abuzer

The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice

Aysun Bay KARABULUT, (İnönü Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Malatya, Türkiye)

Dinçer ÖZGÖR, (İnönü Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Malatya, Türkiye)

Cengiz ARA, (İnönü Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Malatya, Türkiye)

Abuzer DİRİCAN, (İnönü Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Malatya, Türkiye)

Latif KAHRAMAN, (İnönü Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Malatya, Türkiye)

Hilmi YAMAN (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Erciyes Tıp Dergisi

2 1

Yıl: 2009 Cilt: 31 Sayı: 4 Sayfa Aralığı: 305 - 309 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice

Öz:

Amaç: Farelerde kolon karsinogenezisinin oluşumu ve gelişiminin izlenmesinde azoksimetanla oluşturulan kolon karsinogenezisi modeli kullanılmıştır. Bu çalışmadaki amacımız vitamin C'nin fare kalın barsağında azoxymethane ile oluşturulan oksidatif strese karşı antioksidan aktivitesini araştırmaktır.Gereç ve Yöntemler: Bu sebeple 12 haftalık 31.49±3.1 g ağırlığında 30 adet Swiss Albino fare onarlı üç gruba ayrıldı. Birinci ve ikinci gruptaki farelere 7 hafta boyunca haftada bir defa subkütanöz 5 mg/kg azoxymethane verildi. Aynı zaman peryodunda ikinci gruptaki farelere 500 mg/kg vitamin-C intraperitoneal uygulandı. Üçüncü gruptaki farelere kontrol grubu olarak herhangi birşey verilmedi.Bulgular: Vitamin C verilen grupta MDA ve NO seviyesi sadece azoxymethane verilen gruba göre anlamlı derecede daha düşük idi (p=0.031, p<0.001). Vitamin C verilen grupta GSH seviyesi sadece azoksimetan verilen gruba göre anlamlı derecede daha yüksek idi (p=0.004).Sonuç: Bu çalışma; farelerde intraperitonel verilen vitamin C'nin azoxymethane ile oluşturulan oksidatif stresi azaltığını göstermektedir.

Anahtar Kelime: Oksidatif stres Azoksimetan Fare Antioksidanlar Kolon Askorbik asit

Konular: Genel ve Dahili Tıp

Vitamin C'nin farelerin kalın barsağında azoxymethane ile oluşturulan oksidatif stres üzerindeki koruyucu etkisi

Öz:

Purpose: The Azoxymethane model for colon carcinogenesis is often used to study on the initiation and promotion stages of colon carcinogenesis in mice. Our aim in this study was to evaluate the effects of vitamin C on levels of oxidative stress parameters in azoxymethane induced colonic oxidative stress in mice.Material and Methods: In this purpose thirty Swiss albino mice (weighing 31.49±3.1 g), aging 12 weeks were randomly divided into three groups of ten each. Animals of group I and II were treated with weekly doses of subcutaneous 5 mg/kg azoxymethane for 7 weeks, whereas, the mice in group II were further treated with 500 mg/kg vitamin C administered intraperitoneally during the same period. Group III served as the control group.Results: In the vitamin C treated mice, levels of MDA and NO were significantly lower than those of azoxymethane only group (p=0.031, p<0.001). The levels of GSH in the vitamin C treated mice were significantly higher than that of azoxymethane only group (p=0.004).Conclusion: The present study demonstrates that intraperitoneal administration of vitamin C reduces azoxymethane induced oxidative stress in colon of mice.

Anahtar Kelime: Colon Ascorbic Acid Oxidative Stress Azoxymethane Mice Antioxidants

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARABULUT A, ÖZGÖR D, CENGİZ A, Dirican A, KAHRAMAN L, YAMAN H (2009). The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. , 305 - 309.
Chicago	KARABULUT Aysun Bay,ÖZGÖR Dinçer,CENGİZ ARA,Dirican Abuzer,KAHRAMAN Latif,YAMAN Hilmi The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. (2009): 305 - 309.
MLA	KARABULUT Aysun Bay,ÖZGÖR Dinçer,CENGİZ ARA,Dirican Abuzer,KAHRAMAN Latif,YAMAN Hilmi The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. , 2009, ss.305 - 309.
AMA	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. . 2009; 305 - 309.
Vancouver	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. . 2009; 305 - 309.
IEEE	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H "The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice." , ss.305 - 309, 2009.
ISNAD	KARABULUT, Aysun Bay vd. "The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice". (2009), 305-309.

APA	KARABULUT A, ÖZGÖR D, CENGİZ A, Dirican A, KAHRAMAN L, YAMAN H (2009). The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. Erciyes Tıp Dergisi, 31(4), 305 - 309.
Chicago	KARABULUT Aysun Bay,ÖZGÖR Dinçer,CENGİZ ARA,Dirican Abuzer,KAHRAMAN Latif,YAMAN Hilmi The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. Erciyes Tıp Dergisi 31, no.4 (2009): 305 - 309.
MLA	KARABULUT Aysun Bay,ÖZGÖR Dinçer,CENGİZ ARA,Dirican Abuzer,KAHRAMAN Latif,YAMAN Hilmi The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. Erciyes Tıp Dergisi, vol.31, no.4, 2009, ss.305 - 309.
AMA	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. Erciyes Tıp Dergisi. 2009; 31(4): 305 - 309.
Vancouver	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice. Erciyes Tıp Dergisi. 2009; 31(4): 305 - 309.
IEEE	KARABULUT A,ÖZGÖR D,CENGİZ A,Dirican A,KAHRAMAN L,YAMAN H "The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice." Erciyes Tıp Dergisi, 31, ss.305 - 309, 2009.
ISNAD	KARABULUT, Aysun Bay vd. "The protective effect of vitamin C on azoxymethane-induced oxidative stress in colon of mice". Erciyes Tıp Dergisi 31/4 (2009), 305-309.