Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media

polat, bülent; Baki Acar, Duygu; Bastan, Ayhan; ÇOLAK, Armağan; KORKMAZ, Ömer

Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media

Ayhan BAŞTAN, (Ankara Üniversitesi, Ankara, Türkiye)

Bülent POLAT, (Atatürk Üniversitesi, Veteriner Fakültesi, Erzurum, Türkiye)

Duygu Baki ACAR, (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Afyon, Türkiye)

Ömer KORKMAZ, (Harran Üniversitesi, Veteriner Fakültesi, Şanlıurfa, Türkiye)

Armağan ÇOLAK (Atatürk Üniversitesi, Veteriner Fakültesi, Erzurum, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

1 0

Yıl: 2010 Cilt: 34 Sayı: 1 Sayfa Aralığı: 33 - 38 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media

Öz:

Bu çalışmanın amacı, sığır oositlerinin in vitro maturasyonunda EGF’nin en uygun dozunun saptanması ve EGF ile mature edilen oositlerin farklı IVF ve IVC vasatlarında inkübe edildikten sonra kültür vasatlarının embryo gelişimi üzerine etkilerinin araştırılmasıdır. Bu çalışmada mezbahadan elde edilen sığır oositleri rastgele seçilerek 4 gruba ayrıldı. Her bir gruptaki oositler EGF’nin optimum dozunu belirlemek amacıyla EGF’nin farklı dozlarında 24 saat süreyle inkübe edildi (Kontrol: 0 ng/mL, Grup 1: 1 ng/mL, Grup 2: 10 ng/mL ve Grup 3: 100 ng/mL). İnkübasyon sonrası mature olan oositler dondurulmuş boğa sperması ile swim up metodu kullanılarak fertilize edildi. Dört gruptaki fertil oositler ikiye ayrılarak yarısı CR1aa ve diğer yarısı G1.3/G2.3 vasatlarında kültüre edildi. Kültür sonrası embriyolar bölünme (48. saat), 3. günde 5-8 hücreli embriyo ve fertilizasyon sonrası 8. günde blastosist aşamaları açısından incelendi. Maturasyon kriterleri olan kumulus ekspansiyonu ve nükleer maturasyon oranları Grup 1, Grup 2 ve Grup 3’de kontrol grubuna oranla yüksek bulundu (P < 0,01). Ancak farklı EGF dozlarında oosit maturasyonu açısından fark olmadığı tespit edildi. Bölünme oranları, kontrol grubuna göre EGF eklenen gruplarda önemli ölçüde yüksek bulundu (P < 0,01). Blastosist gelişimi açısından, CR1aa ve G1.3/G2.3 kültür vasatlarında inkübe edilen embryolar arasında önemli farklılık saptanmadı (P > 0.1).

Anahtar Kelime: in vitro fertilization epidermal growth factor blastocyst embryos cattle cell culture beef cattle oocyte maturation oocytes determination culture media cows embryonic development

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bastan A, polat b, Baki Acar D, KORKMAZ Ö, ÇOLAK A (2010). Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. , 33 - 38.
Chicago	Bastan Ayhan,polat bülent,Baki Acar Duygu,KORKMAZ Ömer,ÇOLAK Armağan Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. (2010): 33 - 38.
MLA	Bastan Ayhan,polat bülent,Baki Acar Duygu,KORKMAZ Ömer,ÇOLAK Armağan Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. , 2010, ss.33 - 38.
AMA	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. . 2010; 33 - 38.
Vancouver	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. . 2010; 33 - 38.
IEEE	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media." , ss.33 - 38, 2010.
ISNAD	Bastan, Ayhan vd. "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media". (2010), 33-38.

APA	Bastan A, polat b, Baki Acar D, KORKMAZ Ö, ÇOLAK A (2010). Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences, 34(1), 33 - 38.
Chicago	Bastan Ayhan,polat bülent,Baki Acar Duygu,KORKMAZ Ömer,ÇOLAK Armağan Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences 34, no.1 (2010): 33 - 38.
MLA	Bastan Ayhan,polat bülent,Baki Acar Duygu,KORKMAZ Ömer,ÇOLAK Armağan Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences, vol.34, no.1, 2010, ss.33 - 38.
AMA	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences. 2010; 34(1): 33 - 38.
Vancouver	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media. Turkish Journal of Veterinary and Animal Sciences. 2010; 34(1): 33 - 38.
IEEE	Bastan A,polat b,Baki Acar D,KORKMAZ Ö,ÇOLAK A "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media." Turkish Journal of Veterinary and Animal Sciences, 34, ss.33 - 38, 2010.
ISNAD	Bastan, Ayhan vd. "Determination of optimal dose of EGF for bovine oocyte maturation and subsequent in vitro fertilization and culture in two media". Turkish Journal of Veterinary and Animal Sciences 34/1 (2010), 33-38.