Yıl: 2010 Cilt: 34 Sayı: 1 Sayfa Aralığı: 11 - 20 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species

Öz:
An efficient and reproducible protocol has been developed for the in vitro production of an endangered medicinal climber Tylophora indica (Burm.f.) Merr. via leaf explants. Different types of calli produced on benzyladenine (BA) and thidiazuron (TDZ) supplemented Murashige and Skoog (MS) basal medium were selected for shoot induction and somatic embryogenesis studies. Calli when transferred from BA (5 μM) and TDZ (2.5 μM) to MS medium containing BA (5 μM) resulted in high frequency shoot induction (26.8 ± 0.97 shoots/culture) along with somatic embryogenesis (10.20 ± 0.37 embryoids/culture) up to 3 subculture passages. Embryoids transformed into complete plantlets when transferred to growth regulator free half-strength MS medium. Auxin specific pigmented and whitish roots were induced in the microshoots when planted on half-strength MS medium augmented with indole-3-butyric acid (IBA) and α- naphthaleneacetic acid (NAA) respectively. Regenerated plantlets were hardened, acclimatised, and established in soil with 90% survival rate and exhibited normal morphology when compared with parent plants.
Anahtar Kelime: benzyladenine Tylophora indica callus somatic embryogenesis shoot tip culture TDZ organogenesis culture techniques plant growth regulators

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA SAHAI A, SHAHZAD A, ANIS M (2010). High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. , 11 - 20.
Chicago SAHAI Aastha,SHAHZAD Anwar,ANIS Mohammad High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. (2010): 11 - 20.
MLA SAHAI Aastha,SHAHZAD Anwar,ANIS Mohammad High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. , 2010, ss.11 - 20.
AMA SAHAI A,SHAHZAD A,ANIS M High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. . 2010; 11 - 20.
Vancouver SAHAI A,SHAHZAD A,ANIS M High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. . 2010; 11 - 20.
IEEE SAHAI A,SHAHZAD A,ANIS M "High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species." , ss.11 - 20, 2010.
ISNAD SAHAI, Aastha vd. "High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species". (2010), 11-20.
APA SAHAI A, SHAHZAD A, ANIS M (2010). High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turkish Journal of Botany, 34(1), 11 - 20.
Chicago SAHAI Aastha,SHAHZAD Anwar,ANIS Mohammad High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turkish Journal of Botany 34, no.1 (2010): 11 - 20.
MLA SAHAI Aastha,SHAHZAD Anwar,ANIS Mohammad High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turkish Journal of Botany, vol.34, no.1, 2010, ss.11 - 20.
AMA SAHAI A,SHAHZAD A,ANIS M High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turkish Journal of Botany. 2010; 34(1): 11 - 20.
Vancouver SAHAI A,SHAHZAD A,ANIS M High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turkish Journal of Botany. 2010; 34(1): 11 - 20.
IEEE SAHAI A,SHAHZAD A,ANIS M "High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species." Turkish Journal of Botany, 34, ss.11 - 20, 2010.
ISNAD SAHAI, Aastha vd. "High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species". Turkish Journal of Botany 34/1 (2010), 11-20.