Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra

DEMİREL, M. Ayşe; KÜPLÜLÜ, Şükrü

Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra

M. Ayşe DEMİREL, (Ankara Üniversitesi, Veteriner Fakültesi, Ankara, Türkiye)

Şükrü KÜPLÜLÜ (Ankara Üniversitesi, Veteriner Fakültesi, Ankara, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2010 Cilt: 16 Sayı: 2 Sayfa Aralığı: 313 - 318 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra

Öz:

Bu çalışmanın amacı, pyometra olgularında gram negatif bakteriyel endotoksinin görülme sıklığı ve düzeyinin belirlenmesi ve operasyonla birlikte antiendotoksemik tedavi etkinliğinin araştırılmasıdır. Çalışma materyalini pyometra tanısı konulan 30 dişi köpek oluşturdu. Pyometralı köpekler rastgele Grup I (polimiksin E-ampisilin tedavisi olanlar) ve Grup II (ampisilin tedavisi olanlar) olmak üzere iki gruba ayrıldı. Her olgunun uterus, vagina ve idrar kesesinden mikrobiyolojik svap örnekleri alındı. Operasyon öncesinde ve sonrası 7. günde tüm olguların kan endotoksin, serum biyokimya ve hematolojik parametreleri değerlendirildi. Endotoksin düzeyini belirlemek amacıyla jelasyon yöntemine göre çalışan E-toxate® test kitinden yararlanıldı. Gram negatif bakteriler Grup I’de %66, Grup II’de %80 oranında izole edildi. Pyometralı 30 köpeğin uterusundan baskın olarak (%63.4) Escherichia coli izole edildiği saptandı. Operasyon öncesi Grup I (n=10)’e ait olgularda serum toksin düzeyinin 1.2±0.4 EU/ml, Grup II (n=12)’de ise 0.9±0.2 EU/ml olduğu belirlendi. Operasyondan sonraki 3. günde Grup I’de 5 olgunun, Grup II’de 3 olgunun öldüğü görüldü. Yaşayan ve ölen hayvanlarda, ölüm riski ile operasyon öncesi BUN (r=0.539, P<0.01), kreatinin seviyesi (r=0.504, P<0.05), toksin düzeyi (r=0.727, P<0.01) arasında pozitif korelasyon belirlendi. Operasyon sonrası 7. günde Grup I (n=6; 0.14±0.02 EU/ml) ve Grup II (n=11; 0.19±0.01 EU/ml)’de belirlenen toksin düzeylerinin operasyon öncesi değerlerine göre düştüğü (P<0.05) saptandı. Grup I ve Grup II’de operasyon sonrası toksin düzeylerindeki azalmanın önemli olmadığı (P>0.05) görüldü. Operasyon öncesinde ve sonrası 7. günde Grup I’de band nötrofil sayısı arasındaki farklılık önemli bulunurken, Grup II’de ALT, ALP, BUN, kreatinin, alyuvar, akyuvar, hematokrit, band nötrofil ve lenfosit oranı arasındaki farklılığın önemli olduğu (P<0.05) saptandı. Sonuç olarak toksin seviyesi ile BUN ve kreatinin düzeyleri arasında pozitif korelasyon olduğu ve bu değerleri yüksek bulunan olguların yaşayabilirlik oranının düşük olduğu, operasyonla birlikte ampisilin uygulamasının polimiksin E+ampisilin kombinasyonuna göre hipotezin aksine köpeklerde pyometra olgularında daha etkili olduğu kanısına varıldı.

Anahtar Kelime: hematoloji dölyatağı ilaç kombinasyonları dişi köpek alanin aminotransferaz akyuvarlar farmakodinamik alkalin fosfotaz ameliyat sonrası bakım piyometra ampisilin vajina köpek bakteriyel toksinler Escherichia coli kreatinin nötrofil alyuvarlar endotoksinler

Konular: Veterinerlik

Endotoksik pyometralı köpeklerde polimiksin E - ampisilin kombinasyonunun antiendotoksik etkisinin araştırılması

Öz:

The aim of this study was to determine the incidence and levels of the gram negative bacterial endotoxin, and to investigate the effects of antiendotoxemic treatment along with operation. Thirty bitches with pyometra were used in this study. The bitches were randomly allocated into two groups as Group I (treated with polymyxin E-ampisillin) and Group II (treated with ampisillin). Samples for bacteriological examination were taken from vagina, vesica urinaria and uterus of all bitches. Blood samples were collected to evaluate biochemical, haematological tests and serum toxine levels before the operation and on 7 days after operation. Gelation was carried out to determine the gram negative bacterial endotoxin level with E-toxate test kit in accordance with gelation system. Gram negative bacteria were isolated 66% in Group I and 80% Group II. Escherichia coli were dominantly (63.4%) isolated from the uteri of 30 bitches with pyometra. Before the operation, serum toxin levels were found in Group I (n=10) 1.2±0.4 EU/ml and in Group II (n=12) 0.9±0.2 EU/ml. Five bitches in Group I and 3 bitches in Group II died on the third day after operation. Survival rate was a positive correlation with toxin levels (r=0.727; P<0.01) and BUN (r=0.539; P<0.01), creatinin (r=0.504, P<0.05) before operation in recovered and dead bitches. Serum toxin levels were not different between Group I (n=6; 0.14±0.02 EU/ml) and Group II (n=11; 0.19±0.01 EU/ml) 7 days after operation. Difference of toxin levels in both groups were different (P<0.05) between the preoperation period and the 7th day after operation. Before the operation and on the 7th day after the operation, in Group I difference between band neutrophil count and in Group II ALT, ALP, BUN, creatinin, RBC, WBC, Hct, band neutrophil and lenphocyte value were found significant (P<0.05). As a result, there was a positive correlation between toxin levels and BUN-creatinin values. Also, the cases that have the increased BUN and creatinin levels along with high toxin levels were trended towards to low survival rate. After operation, in cases that performed with ampicillin were acquired more effective results contrary to the hipothesis according to polymyxin E+ampicillin combination in bitches with pyometra.

Anahtar Kelime: pyometra ampicillin vagina dogs bacterial toxins Escherichia coli creatinine neutrophils erythrocytes endotoxins haematology uterus drug combinations bitches alanine aminotransferase leukocytes pharmacodynamics alkaline phosphatase postoperative care

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Dabrowski R, Wawron W, Kostro K: Changes in CRP, SAA and haptoglobin produce in response to ovariohysterectomy in healthy bitches and those with pyometra. Theriogenology, 67, 321-327, 2007.
2. Hagman R, Kindahl H, Lagerstedt AS: Pyometra in bitches induces elevated plasma endotoxin and prostaglandin F2α metabolite levels. Acta Vet Scand, 47, 55-68. 2006.
3. Nelson RW, Feldman EC: Pyometra in the bitch. In, Morrow DA (Ed): Current Therapy in Theriogenology. pp. 484-489. WB Saunders Company, Philadelphia, 1986.
4. Noakes DE, Dhaliwal GK, England GC: Cystic endometrial hyperplasia/pyometra in dogs: A review of the causes and pathogenesis. J Reprod Fertil, 57, 395-406, 2001.
5. Threlfall WR: Diagnosis and medical management of pyometra. Sem Vet Med Surg (Small Anim), 10, 21-29, 1995.
6. Okano S, Tagawa M, Takase K: Relationship of the blood endotoxin concentration and prognosis in dogs with pyometra. J Vet Med Sci, 60, 1265-1267, 1998.
7. Johnston SD, Kustritz MR, Olson PNS: Disorders of the canine uterus and uterine tubes (oviducts). In, Ray K, Denise L (Eds): Canine and Feline Theriogenology, pp. 206-225. WB Saunders Company, 2001.
8. Hagman R, Kühn I: Escherichia coli strains isolated from the uterus and urinary bladder of bitches suffering from pyometra: Comparison by restriction enzyme digestion and pulsed-field gel electrophoresis. Vet Microbiol, 84, 143-153, 2002.
9. Hagman R, Kindahl H, Fransson BA, Bergström A, Ström Holst B, Lagerstedt AS: Differentiation between pyometra and cystic endometrial hyperplasia/mucometra in bitches by prostaglandin F2α metabolite analysis. Theriogenology, 66, 198-206, 2006.
10. Dhaliwal GK: Uterine bacterial flora and uterine lesions in bitches with cystic endometrial hyperplasia (pyometra). Vet Rec, 143, 659-661, 1998.
11. Chen YMM, Wright PJ, Lee CS, Browning G: Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches. Vet Microbiol, 94, 5769, 2003.
12. England G: Infertility in the bitch and queen. In, Noakes DE, Parkinson TJ, England GCW (Eds): Veterinary Reproduction and Obstetrics. pp. 639-671. WB Saunders Harcourt Pub. Lim, 2001.
13. Wadas B, Kühn I, Lagerstedt AS, Jansson P: Biochemical phenotypes of Escherichia coli in dogs: Comparison of isolates isolated from bitches suffering from pyometra and urinary tract infection with isolates from faeces of healthy dogs. Vet Microbiol, 52, 293-300, 1996.
14. Piens K, De Schepper J, Depelsmaecker K: Bilirubinuria without hyperbilirubinaemia in bitches with pyometra. Vlaams Diergeneeskundig Tijdschrift, 65, 31-33, 1996.
15. Braun JP, Lefebvre HP, Watson ADJ: Creatinine in the dog: A review. Vet Clin Pathol, 32, 162-179, 2003.
16. Fransson B: Systemic inflammatory response in canine pyometra. Doctoral Thesis. Swedish University of Agricultural Sciences, Uppsala, 2003.
17. Hagman R, Greko C: Antimicrobial resistance in Escherichia coli isolated from bitches with pyometra and from urine samples from other dogs. Vet Rec, 157, 193-197, 2005.
18. Wernicki A, Krzyzanowski J, Puchalski A: Characterization of Escherichia coli strains associated with canine pyometra. Pol J Vet Sci, 5, 51-56, 2002.
19. Kida K, Baba E, Torri R, Kawate N, Hatoya S, Wijewardana V, Sugiura K, Sawada T, Tamada H, Inaba T: Lactoferrin expression in the canine uterus during the estrous cycle and with pyometra. Theriogenology, 66, 1325-1333, 2006.
20. Bigliardi E, Parmigiani E, Cavirani S, Luppi A, Bonati L, Corradi A: Ultrasonography and cystic hyperplasia-pyometra complex in the bitch. Reprod Dom Anim, 39, 136-140, 2004.
21. Hagman R: New aspect of canine pyometra. Doctoral Thesis. Swedish University of Agricultural Sciences, Uppsala, 2004.
22. Blendinger K, Bostedt H: The age and stage of estrus in bitches with pyometra. Statistical inquiry and interpretive study of the understanding of variability. Tierarztl Prax, 19, 307-310, 1991.
23. Feldman EC, Nelson RW: Cystic endometrial hyperplasiapyometra complex. In, Canine and Feline Endocrinology and Reproduction. W.B. Saunders Co, Tokyo. p. 605-619, 1996.
24. Smith F: Canine pyometra. Theriogenology, 66, 610-612, 2006.
25. Egenvall A, Hagman R, Bonnett BN, Hedhammar A, Olson P, Lagerstedt AS: Breed risk of pyometra in insured dogs in Sweden. J Vet Intern Med, 15, 530-538, 2001.
26. Jayaprakash R, Sathiamoorthy T, Sureshkumar R: Incidence of pyometra in bitches: A retrospective study of 249 cases. Tamilnadu J Vet Anim Sci, 3, 164-165. 2007.
27. Fransson B, Lagerstedt AS, Hellmen E, Jonsson P: Bacteriological findings, blood chemistry profile and plasma endotoxin levels in bitches with pyometra or other uterine diseases. Zentralbl Veterinarmed A, 44, 417-426, 1997.
28. De Schepper J, Van Der Stock J, Capiau E: The characteristic pattern of aspartate aminotransferase and alanine amino-transferase in the bitch the cystic hyperplasia-pyometra complex: Effect of medical or surgical treatment. Vet Res Commun, 11, 65-75, 1987.
29. Bush BM: Enzymes. In, Interpretation of laboratory results for small animal clinicians. ISBN 0-632-03259-6. 4th ed. Blackwell Scence. pp. 311-350, 1996.
30. Sodikoff CH: Serum chemistry test. In, Laboratory Profiles of Small Animal Diseases. pp. 5-31, 2001.
31. Sevelius E, Tidholm A, Tolling KT: Pyometra in the dog. J Am Anim Hos Assoc, 26, 33-38, 1990.
32. Heiene R, Moe L, Molmen G: Calculation of urinary enzyme excretion, with renal structure and function in dogs with pyometra. Res Vet Sci, 70, 129-137, 2001.
33. Sano J, Oguma K, Kano R, Tsumagarı S, Hasegawa A: Decreased apoptotic polymorphonuclear leukocyte rate in dogs with pyometra. J Vet Med Sci, 66, 103-105, 2004.
34. Hagman R, Lagerstedt AS, Fransson BA, Bergström A, Häggström J: Cardiac troponin I levels in canine pyometra. Acta Vet Scand, 49, 1-6, 2007.
35. Hoffman WD, Danner RL, Quezado ZMN, Banks SM, Elin RJ, Hosseini JM, Natanson C: Role of endotoxemia in cardiovascular dysfunction and lethality: Virulent and nonvirlent Escherichia coli challenges in a canine model of septic shock. Infect Immun, 64, 406412, 1996.
36. Şentürk S: Evaluation of the anti-endotoxic effects of polymyxin-E (colistin) in dogs with naturally occurred endotoxic shock. J Vet Pharmacol Therap, 28, 57-63, 2005.
37. Stone EA, Littman MP, Robertson JL, Bovee KC: Renal dysfunction in dogs with pyometra. J Am Vet Med Assoc, 193, 457-464, 1988.

APA	DEMİREL M, KÜPLÜLÜ Ş (2010). Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. , 313 - 318.
Chicago	DEMİREL M. Ayşe,KÜPLÜLÜ Şükrü Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. (2010): 313 - 318.
MLA	DEMİREL M. Ayşe,KÜPLÜLÜ Şükrü Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. , 2010, ss.313 - 318.
AMA	DEMİREL M,KÜPLÜLÜ Ş Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. . 2010; 313 - 318.
Vancouver	DEMİREL M,KÜPLÜLÜ Ş Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. . 2010; 313 - 318.
IEEE	DEMİREL M,KÜPLÜLÜ Ş "Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra." , ss.313 - 318, 2010.
ISNAD	DEMİREL, M. Ayşe - KÜPLÜLÜ, Şükrü. "Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra". (2010), 313-318.

APA	DEMİREL M, KÜPLÜLÜ Ş (2010). Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(2), 313 - 318.
Chicago	DEMİREL M. Ayşe,KÜPLÜLÜ Şükrü Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.2 (2010): 313 - 318.
MLA	DEMİREL M. Ayşe,KÜPLÜLÜ Şükrü Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.2, 2010, ss.313 - 318.
AMA	DEMİREL M,KÜPLÜLÜ Ş Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(2): 313 - 318.
Vancouver	DEMİREL M,KÜPLÜLÜ Ş Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(2): 313 - 318.
IEEE	DEMİREL M,KÜPLÜLÜ Ş "Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.313 - 318, 2010.
ISNAD	DEMİREL, M. Ayşe - KÜPLÜLÜ, Şükrü. "Investigation on the antiendotoxic effect of the combination of polymyxin E and ampicillin in dogs with endotoxic pyometra". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/2 (2010), 313-318.