Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs

Yuksek, Nazmi; altuğ, nuri; AĞAOĞLU, Zahid Tevfik

Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs

Nuri ALTUĞ, (Mustafa Kemal Üniversitesi, Veteriner Fakültesi, Hatay, Türkiye)

Nazmi YÜKSEK, (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Van, Türkiye)

Zahid Tevfik AĞAOĞLU (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Van, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

16 2

Yıl: 2010 Cilt: 16 Sayı: 3 Sayfa Aralığı: 405 - 412 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs

Öz:

Bu çalışmada köpeklerde aşılama sonrası lenfosit alt tipleri, hematolojik parametreler ve immunglobulin konsantrasyonları üzerinde Aloe vera ve β-Glucan’ın etkilerinin değerlendirilmesi amaçlandı. Yirmi sokak köpeği kullanıldı. Hayvanlar beş gruba ayrıldı. Grup 1’deki köpeklere sadece karma aşı uygulandı. Grup 2 ve 3’teki köpeklere sırasıyla sadece Aloe vera ve β-Glucan uygulandı. Grup 4 ve 5’teki köpeklere ise karma aşıya ilaveten Aloe vera ve β-Glucan uygulandı. Aşılama öncesi ve aşılama sonrası (AS) kan örnekleri alındı. Platelet (PLT) sayıları grup 1’de AS azalmasına rağmen, diğer tüm gruplarda (grup 2, 3, 4, 5) arttı. Total lökosit (WBC) ve periferal kan agranülosit (PBML) sayıları (tüm gruplarda), periferal kan granülosit (PBPL) sayıları (grup 1, 2, 4, 5), nötrofil (grup 1, 4, 5), monosit (grup 2, 3, 5) ve hematokrit (PCV) oranları (grup 2, 3) ile hemoglobin (HGB) konsantrasyonları (grup 3) AS’da arttı. Fakat, lenfosit oranları AS’da tüm gruplarda değişmedi. CD8 T lenfosit oranları tüm gruplarda AS 7 ve 14. günlerde arttı. CD3 T lenfosit oranları grup 1’de AS 7. günde artmasına rağmen, CD4 T lenfosit ve B lenfosit oranları AS periyotta değişmedi. Fakat, CD3 ve CD4 T lenfosit oranları diğer tüm gruplarda AS 7. ve 14. günlerde arttı. Üstelik, B lenfosit oranları AS’da grup 1 hariç diğer tüm gruplarda arttı. Ayrıca, B lenfosit oranları grup 2 ve 3’de sadece AS 7. günde artarken, grup 4 ve 5’de AS hem 7. hemde 14. günde arttı. Serum IgM konsantrasyonları grup 1’de sadece AS 7. günde artarken, grup 2 ve 3’de değişmedi. Serum IgG konsantrasyonları grup 1, 2 ve 3’de sadece AS 14. günde arttı. Fakat, serum IgM ve IgG konsantrasyonları grup 4 ve 5’de AS 7. ve 14. günde arttı. Köpeklerde aşılama sonrası Aloe vera ve β-Glucan uygulamasının hücresel ve hümoral immun cevapların her ikisini de stimüle edebileceği ve PLT sayıları üzerinde restoratif etkili olabileceği kanısına varıldı.

Anahtar Kelime: lenfositler bağışık yanıt hematoloji aşılama (hayvan) akyuvarlar T lenfositleri Aloe barbadensis tıbbi bitkiler hücresel bağışıklık humoral bağışıklık bağışıklık uyarımı köpek hemoglobin IgM sokak hayvanları bitki bileşimi monosit nötrofil B lenfosit hematokrit

Konular: Veterinerlik

Köpeklerde karma aşı ile aşılama sonrası hücresel ve humoral immun cevaplar üzerinde Aloe vera ve β-Glucan’ın immunostimülatör etkileri

Öz:

The aim of this study was to evaluate the effects of Aloe vera and β-Glucan on lymphocyte subsets, haematological parameters and immunglobulin concentrations following vaccination in dogs. For this purpose, totally 20 street dogs were used. The animals were allocated into five groups. Group 1 consisted of only polyvalent vaccine applied dogs. Group 2 and 3 were only Aloe vera and β-Glucan applied dogs, respectively. Group 4 and 5 were consisted of polyvalent vaccine in addition to Aloe vera and β-Glucan applied dogs, respectively. Blood samples were collected before vaccination and after vaccination (AV). Although platelet (PLT) counts in group 1 decreased AV, in all other groups (group 2, 3, 4, 5) it increased. White blood cell (WBC) and peripheral blood mononuclear lymphocyte (PBML) counts (in all groups), peripheral blood polymorphonuclear lymphocyte (PBPL) counts (group 1, 2, 4, 5), neutrophil (group 1, 4, 5), monocyte (group 2, 3, 5), packed cell volume (PCV) ratios (group 2 and 3) and haemoglobin (HGB) concentrations (group 3) increased AV. But, lymphocyte ratios in all groups did not change AV. CD8 T lymphocyte ratios in all groups increased at $7^{th}$ and $14^{th}$ days AV. Although CD3 T lymphocyte ratios in group 1 increased at $7^{th}$ day AV, CD4 T and B lymphocytes ratios did not change AV period. But, both CD3 and CD4 T lymphocytes ratios in all other groups increased at $7^{th}$ and $14^{th}$ days AV. Moreover, B lymphocytes ratios in all groups except for group 1 increased AV. On the other hand, B lymphocytes ratios in group 2 and 3 increased only $7^{th}$ day AV, while this increase in group 4 and 5 were present on both $7^{th}$ and $14^{th}$ days AV. Serum IgM concentrations in group 1 increased only $7^{th}$ day AV, whereas its levels did not change in group 2 and 3. Serum IgG concentrations in group 1, 2 and 3 increased only $14^{th}$ day AV. But, serum IgM and IgG concentrations in group 4 and 5 increased at $7^{th}$ and $14^{th}$ days AV. Administration of Aloe vera and β-Glucan after vaccination in dogs may stimulate both cellular and humoral immun responses. Additionally, it might have restorative effect on PLT counts.

Anahtar Kelime: dogs haemoglobin IgM stray animals plant composition monocytes neutrophils B lymphocytes haematocrit lymphocytes immune response haematology vaccination leukocytes T lymphocytes Aloe barbadensis medicinal plants cell mediated immunity humoral immunity immunostimulation

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	altuğ n, Yuksek N, AĞAOĞLU Z (2010). Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. , 405 - 412.
Chicago	altuğ nuri,Yuksek Nazmi,AĞAOĞLU Zahid Tevfik Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. (2010): 405 - 412.
MLA	altuğ nuri,Yuksek Nazmi,AĞAOĞLU Zahid Tevfik Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. , 2010, ss.405 - 412.
AMA	altuğ n,Yuksek N,AĞAOĞLU Z Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. . 2010; 405 - 412.
Vancouver	altuğ n,Yuksek N,AĞAOĞLU Z Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. . 2010; 405 - 412.
IEEE	altuğ n,Yuksek N,AĞAOĞLU Z "Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs." , ss.405 - 412, 2010.
ISNAD	altuğ, nuri vd. "Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs". (2010), 405-412.

APA	altuğ n, Yuksek N, AĞAOĞLU Z (2010). Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(3), 405 - 412.
Chicago	altuğ nuri,Yuksek Nazmi,AĞAOĞLU Zahid Tevfik Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.3 (2010): 405 - 412.
MLA	altuğ nuri,Yuksek Nazmi,AĞAOĞLU Zahid Tevfik Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.3, 2010, ss.405 - 412.
AMA	altuğ n,Yuksek N,AĞAOĞLU Z Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(3): 405 - 412.
Vancouver	altuğ n,Yuksek N,AĞAOĞLU Z Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(3): 405 - 412.
IEEE	altuğ n,Yuksek N,AĞAOĞLU Z "Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.405 - 412, 2010.
ISNAD	altuğ, nuri vd. "Immunostimulatory effects of Aloe vera and β-Glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/3 (2010), 405-412.