LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens

YURDUSEV, Nevzat; FINDIK, ARZU; BÜYÜKTANIR YAŞ, ÖZLEM

LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens

Arzu FINDIK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

ÖZLEM BÜYÜKTANIR YAŞ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Nevzat YURDUSEV (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2010 Cilt: 16 Sayı: 3 Sayfa Aralığı: 487 - 492 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens

Öz:

Salmonella B ve D serogruplarına ait kanatlı salmonellosis’i halk sağlığı yönünden ve kanatlı endüstrisindeki ekonomik kayıplar nedeniyle büyük öneme sahiptir. Kanatlı salmonellozisinin etkin tanısı amacıyla, SE lipopolisakkarid (LPS) ve saflaştırılmış flagellin (Flg) tanı antijenlerine dayalı iki indirekt ELISA modeli geliştirilmesinde ticari Salmonella Enteritidis (SE) ELISA kiti ile değerlendirilmiş toplam 126 tavuk serumu kullanıldı. Optimizasyon sonrası, 154 serum indirekt LPS-ELISA ve 263 serum Flg-ELISA ile test edildi. Ticari LPS-ELISA kiti ile karşılaştırıldığında, indirekt LPS- ve Flg-ELISA’ların anti-Salmonella antikor tespit performansları yüksek ve benzer bulundu. Bununla birlikte, sınırlı sayıda serumun Flg- ve LPS-ELISA’da farklılık gösterdiği belirlendi. Bu uyumsuz serumların SE bakteri lizatı ile reaktif olduğu, buna karşın yalnızca LPS veya Flg antijenini tanıdıkları Western Blot analiziyle gösterildi. Bu çalışmadan, LPS- ve Flg-ELISA’nın eşzamanlı kullanımıyla kanatlılara özgü salmonellozisin flagellalı bakteri kökenli zoonotik Salmonella enfeksiyonlarından ayırıcı tanısının yapılabileceği ve Western blot modellerinin doğrulayıcı serolojik test olarak kullanılabileceği sonucuna varıldı.

Anahtar Kelime: bağışıksal tanı Salmonella enteritidis teşhis zoonoz halk sağlığı kümes hayvanları salmonelloz antikor antijenler seroloji tanı teknikleri ELISA

Konular: Veterinerlik

Tavuklarda Salmonella enfeksiyonlarının serolojik tanısı ve doğrulanmasına yönelik LPS ve flagelline dayalı modeller

Öz:

Avian salmonellosis due to Salmonella B and D serogroups has a great importance because of the public health and the economical losses in poultry industry. For accurate diagnosis of avian salmonellosis, a total of 126 chicken sera evaluated with a commercial Salmonella Enteritidis (SE) ELISA kit have been used to develop two indirect ELISA models using lipopolysaccharide (LPS) and purified flagellin (Flg) of SE as diagnostic antigens. After optimization, 154 and 263 sera were tested with indirect LPS- and Flg-ELISA, respectively. The performance of both indirect LPS- and Flg-ELISA was found high and similar for detecting anti-Salmonella antibody in comparison with a commercial LPS-ELISA kit. Nevertheless, a limited number of chicken sera were found discordant between Flg- and LPS-ELISAs. Western-blot analyses of the discordant sera demonstrated that they were found reactive with bacterial lysate of SE but only recognized LPS or Flg antigen. It was concluded from this study that the simultaneous use of both LPS- and Flg-ELISAs would allow differential diagnosis of avian specific salmonellosis from zoonotic Salmonella infections due to flagellated bacteria and Western blot models can be used as confirmatory serological test.

Anahtar Kelime: antibodies antigens serology diagnostic techniques ELISA immunodiagnosis Salmonella enteritidis diagnosis zoonoses public health poultry salmonellosis

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	FINDIK A, BÜYÜKTANIR YAŞ Ö, YURDUSEV N (2010). LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. , 487 - 492.
Chicago	FINDIK ARZU,BÜYÜKTANIR YAŞ ÖZLEM,YURDUSEV Nevzat LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. (2010): 487 - 492.
MLA	FINDIK ARZU,BÜYÜKTANIR YAŞ ÖZLEM,YURDUSEV Nevzat LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. , 2010, ss.487 - 492.
AMA	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. . 2010; 487 - 492.
Vancouver	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. . 2010; 487 - 492.
IEEE	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N "LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens." , ss.487 - 492, 2010.
ISNAD	FINDIK, ARZU vd. "LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens". (2010), 487-492.

APA	FINDIK A, BÜYÜKTANIR YAŞ Ö, YURDUSEV N (2010). LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(3), 487 - 492.
Chicago	FINDIK ARZU,BÜYÜKTANIR YAŞ ÖZLEM,YURDUSEV Nevzat LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.3 (2010): 487 - 492.
MLA	FINDIK ARZU,BÜYÜKTANIR YAŞ ÖZLEM,YURDUSEV Nevzat LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.3, 2010, ss.487 - 492.
AMA	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(3): 487 - 492.
Vancouver	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(3): 487 - 492.
IEEE	FINDIK A,BÜYÜKTANIR YAŞ Ö,YURDUSEV N "LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.487 - 492, 2010.
ISNAD	FINDIK, ARZU vd. "LPS and Flagellin-based models for serological screening and confirmation of Salmonella infections in chickens". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/3 (2010), 487-492.