The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips

SINGH, ATUL; ROY, Bijoy Krishna; Kumar, Sanjay; ARYA, Shashi Kiran

The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips

Sanjay KUMAR, (Department, of Botany, Nagaland Univercity Headquarter, Lumami, Nagaland-798601, India)

Shashi Kiran ARYA, (Center of Advance Study in Botany, Banaras Hindu Univercity Varanasi,221005, India)

Bijoy Krishna ROY, (Center of Advance Study in Botany, Banaras Hindu Univercity Varanasi,221005, India)

Atul Kumar SINGH (College of Biotechnology and Allied Science, Allahabad Agricultural Institute, Deemed Univercity Allahabad-211007, India)

Turkish Journal of Biology

1 0

Yıl: 2010 Cilt: 34 Sayı: 1 Sayfa Aralığı: 55 - 66 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips

Öz:

Üç buğday (Triticum aestivum L.) varyetesi HUW 234, HUW 468 ve HUW 533 ‘nin mitotik bozukluklar ve kromozomlar üzerine 2,4-diklorofenoksi asetik asit ve izoproturon herbisitinin etkisi çalışılmıştır. Önceden ıslatılmış tohumlar 50-1200 ppm lik herbisit ile muamele edilmiştir. Hem 2,4-D hem de izoproturon mitoz üzerine oldukça inhibitor etkisi olduğu ve erken hareket, stickiness, kromozom köprüleri ve anafaz ve telefazda fragmentler gibi kromozom bozukluklarına sebep olduğu gözlenmiştir. Kromozom anormallikleri her herisit için bireysel veya her ikisnin karşımında en yüksek dozda çok fazla gözlenmiştir. İzoproturon daha toksiktir. Bütün varyetelerde en fazla kromozom anarmalliğine raslanmıştır. Her iki herbisit doza bağlı mitotik indeks (MI) etkisine ve nisbi bozukluk oranına (RAR) neden olmuştur. Parametreler HUW 468 buğday varyetesinin kullanılan herbisitlere karşı daha hassas olduğunu göstermiştir.

Anahtar Kelime: defekt kromozomlar Triticum aestivum kromozom hataları yabancı ot öldürücüler mitoz mitotik indeks kök uçları 2,4- diklorofenoksiasetik asit isoproturon büyüme oranı buğday

Konular: Biyoloji

Buğday kök uçlarına (Triticum aestivum L.) 2,4-diklorofenoksi asetik asit ve izoproturon herbisitinin etkisi

Öz:

The effects of the herbicides 2,4-dichlorophenoxy acetic acid and isoproturon on 3 wheat (Triticum aestivum L.) varieties (HUW 234, HUW 468, and HUW 533) were studied with regards to mitotic abnormalities and chromosomal behavior. Pre-soaked seeds were treated with both herbicides at concentrations of 50-1200 ppm. Both 2,4-D and isoproturon were highly mito-inhibitory and induced chromosomal abnormalities, such as precocious movement, stickiness, and chromosome bridges, with and without laggards and fragments at the anaphase and telophase. The frequency of chromosomal anomalies in almost all the targets used was high at the maximum dose of both herbicides, individually as well as in combination. Isoproturon was more toxic, as it resulted in high-level chromosomal damage in all the varieties. Both herbicides had a dose-dependent impact on the mitotic index (MI) and relative abnormality rate (RAR). These parameters collectively indicate that variety HUW 468 was more susceptible to the mito-depressive and chromotoxic action of the 2 herbicides.

Anahtar Kelime: root tips 2,4- dichlorophenoxyacetic acid isoproturon growth rate wheat defects chromosomes Triticum aestivum chromosome aberrations herbicides mitosis mitotic index

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Weed Science Society of America. DCPA. In: Herbicide handbook of the Weed Science Society of America, fifth edition. Weed Science Society of America, Champaign, Illinois, USA; pp. 144-146: 1983a.
2. Weed Science Society of America. Propham. In: Herbicide handbook of the Weed Science Society of America, fifth edition. Weed Science Society of America, Champaign, Illinois, U.S.A.; pp. 413-419: 1983b.
3. Chauhan LKS, Sundraraman V. Effect of substituted ureas on plant cells I. Cytological effects of isoproturon on the root meristem cells of Allium cepa. Cytologia 55: 91-98, 1990.
4. Wuu KD, Grant WF. Morphological and somatic chromosomal aberrations induced by pesticides in barley (Hordeum vulgare). Can J Genet Cytol 8: 481-501, 1966.
5. İnceer H, Ayaz S, Beyazoğlu O et al. Cytogenetic effects of copper chloride on the root tip cells of Helianthus annus L. Turk J Biol 27: 43-46, 2003.
6. Hilmann G, Ruthmann A. Effect of mitotic inhibitors on the ultrastructure of root meristem cells. Planta 155: 124-132, 1982.
7. Holmsen JD, Hess FD. Growth inhibition and disruption of mitosis by DCPA in oat (Avena sativa) roots. Weed Sci 32: 732- 738, 1984.
8. Bovery RW, Meyer RE. Effects of 2, 4, 5-T, Triclopyr and 3, 6- Dichloropicolinic acid on crop seedlings. Weed Sci 29: 256-261, 1981.
9. DePrado R, Scalla R, Gaillardon P. Differential toxicity of simazine and diuron to Torilus arvensis and Lolium rigidum. Weed Res 30: 213-221, 1990.
10. Lerda D. The effects of lead on Allium cepa L. Mutat Res 281: 89-92, 1992.
11. Yoshida Y, Nakamura K, Hiura A. Contraction of chromosomes and depression of RNA synthesis by isopropyl N-(3- chlorophenyl) carbamate (CIPC) in Vicia faba root tip cells. Cytologia 48: 707-714, 1983.
12. Strachan SD, Hess FD. The biochemical mechanism of the action of the dinitroaniline herbicide oryzaline. Pestic Biochem Physiol 20: 141-150, 1983.
13. Coss RA, Pickett-Heaps JD. The effects of isopropyl N-phenyl carbamate on the green alga Oedogonium cardiacum I. Cell division. J Cell Biol 63: 84-98, 1974.
14. Jackson WT. Regulation of mitosis II. Interaction of isopropyl N-phenylcarbamate and melatonin. J Cell Sci 5: 745-755, 1969.
15. Lignowski EM, Scott EG. Effect of trifluralin on mitosis. Weed Sci 20: 267-270, 1972.
16. Delcourt A, Deysson G. Effects de la trifluralin sur les meristemes radiculaires d’ Allium sativum L. Cytologia 41: 75- 84, 1976.
17. Vaughn KC, Lehnen LP Jr. Mitotic disrupter herbicides. Weed Sci 39: 450-457, 1991.
18 Gentner WA, Burk LG. Gross morphological and cytological effects of nitralin on corn root tips. Weed Sci 16: 259-260, 1968.
19. Darwent AL, Elliot CR, Clark JM. Effect of 2, 4-D, Dicamba and 2, 4, 5-T on creeping red fescue seed production. Can J Plant Sci 57: 451-456, 1977.
20. Bovey RW. Hormone like herbicides in weed control. Econ Bot 25: 385-400, 1971.
21. Gichner T, Gaul H, Omura T. The influence of post- treatment, washing and redrying of barley seeds on the mutagenic activity of N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea. Radiat Bot 8: 499-507, 1968.
22. Rekha K, Kak SN, Langer A. Cytological abnormalities in Artemisia pollens Wall. Induced by gamma rays. Indian J Forestry 27: 355-359, 2004.
23. Egami N, Hyodo-Taguchi Y. Dominant lethal mutation rates in fish Oryzias latipes irraditated at various stages of gametogenesis In: Schroder JH. ed. Genetics and Mutagenesis in Fish, Springer Verlag, pp. 75-81, 1973.
24. Kumar G, Kumar R. Chromotoxic and mito-inhibitory effects of pesticides in Trigonella foenum-graecum L. J Cytol Genet 1: 11- 15, 2000.
25. Merlin G, Nuret F, Ravanel P et al. Mitosis inhibition by a N-(1, 1-dimethylpropyl) benzamide series. Phytochem 26: 1567-1571, 1987.
26. Amer S, Ali EM. Cytological effects of pesticides- V. Effects of some herbicides on Vicia faba. Cytologia 39: 633-643, 1974.
27. Morrison JW. Cytological effects of the herbicides avadex. Can J Plant Sci 42: 78-81, 1962.
28. Topaktas M, Rencuğullari E. Cytogenetic effects of herbicides gesegard and igran in barley. Cytologia 56: 419-424, 1991.
29. Elkhodary S, Habib A, Haliem A. Cytological effect of the herbicide garlon-4 on root mitosis of Allium cepa. Cytologia 54: 465-472, 1989.
30. Badr A. Mitodepressive and chromotoxic activities of two herbicides in Allium cepa. Cytologia 48: 451-457, 1983.
31. Lehnen LP, Vaughan MA, Vaughn KC. Terbutol affects spindle microtubule organizing centers. J Exp Bot 41: 537-546, 1990.
32. Vaughn KC. Cytological studies of dinitroaniline-resistant Eleucine. Pestic Biochem Physiol 26: 66-74, 1986.
33. Tissut M, Nurit F, Ravanel P et al. Herbicidal modes of action depending on substitution in a phenylcarbamate series. Physiol Veg 24: 523-535, 1986.
34. Badr A, Elkington TT. Antimitotic and chromotoxic activities of isoproturon in Allium cepa and Hordeum vulgare. Environ and Exp Bot 22: 265-270, 1982.
35. Johanson NG, Muzik TJ. Some effect of 2, 4-D on wheat yield and root growth. Bot Gaz 122: 188-194, 1961.
36. Gaulden ME. Hypothesis: Some mutagens directly alter specific chromosomal proteins (DNA topoisimerase II and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations. Mutagenesis 2: 357-365, 1987.
37. Evans HJ, Scott D. The induction of chromosome aberrations by N- mustard and its dependence on DNA synthesis. Proc R Soc B Biol 173: 491-512, 1969.
38. Kumar G, Singh V. Studies in M1 and M2 generations of azide induced medium-strong desynaptic mutant of pearl millets. The Nucleus 47: 159-163, 2004.
39. Koduru PRK, Rao MK. Cytogenetics of synaptic mutants in higher plants. Theor Appl Genet 59: 197-214, 1981.
40. Morejohn LC, Fosket DE. Inhibition of plant microtubule polymerization in vitro by the phosphoric amide herbicide amiprophosmethyl. Sci 224: 874-876, 1984.
41. Zeerak NA. Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonate in brinjal (Solanum melongena L.). J Nuclear Agri Biol 21: 84-87, 1992.
42. Grant WF. The present status of higher plant bioassays for the detection of environmental mutagens. Mutat Res 310: 175-185, 1994.
43. World Health Organisation. Guide to short term tests for detecting mutagenic and carcinogenic chemicals. Environ Health Criteria 51: Geneva, pp 208, 1985.
44. Grant WF. Chromosomal aberration in plants as a monitoring system. Environ Hlth Persp 27: 37-43, 1978.
45. Oraler G, Gözukırmızı N, Olgun A. Mutagenic effects of some pesticides in different organisms. Doğa Bilim Dergisi 8: 105- 111, 1984.

APA	Kumar S, ARYA S, ROY B, SINGH A (2010). The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. , 55 - 66.
Chicago	Kumar Sanjay,ARYA Shashi Kiran,ROY Bijoy Krishna,SINGH ATUL The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. (2010): 55 - 66.
MLA	Kumar Sanjay,ARYA Shashi Kiran,ROY Bijoy Krishna,SINGH ATUL The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. , 2010, ss.55 - 66.
AMA	Kumar S,ARYA S,ROY B,SINGH A The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. . 2010; 55 - 66.
Vancouver	Kumar S,ARYA S,ROY B,SINGH A The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. . 2010; 55 - 66.
IEEE	Kumar S,ARYA S,ROY B,SINGH A "The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips." , ss.55 - 66, 2010.
ISNAD	Kumar, Sanjay vd. "The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips". (2010), 55-66.

APA	Kumar S, ARYA S, ROY B, SINGH A (2010). The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology, 34(1), 55 - 66.
Chicago	Kumar Sanjay,ARYA Shashi Kiran,ROY Bijoy Krishna,SINGH ATUL The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology 34, no.1 (2010): 55 - 66.
MLA	Kumar Sanjay,ARYA Shashi Kiran,ROY Bijoy Krishna,SINGH ATUL The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology, vol.34, no.1, 2010, ss.55 - 66.
AMA	Kumar S,ARYA S,ROY B,SINGH A The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology. 2010; 34(1): 55 - 66.
Vancouver	Kumar S,ARYA S,ROY B,SINGH A The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology. 2010; 34(1): 55 - 66.
IEEE	Kumar S,ARYA S,ROY B,SINGH A "The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips." Turkish Journal of Biology, 34, ss.55 - 66, 2010.
ISNAD	Kumar, Sanjay vd. "The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips". Turkish Journal of Biology 34/1 (2010), 55-66.