Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones
Yıl: 2010 Cilt: 34 Sayı: 1 Sayfa Aralığı: 75 - 80 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones
Öz: Bu araştırmada, üç değişik çilek klonuna ait yaprak sapı, boğum ve stolon parçalarından kallus elde edilmesi üzerine oksin ve sitokininin etkileri ortaya konulmuştur. Denenen otuz iki besin ortamı kombinasyonu arasında 4,0 mg/L NAA + 1,5 mg/L IBA, tüm eksplant tiplerinde en yüksek kallus oluşum oranını vermiştir. Kallustan adventif sürgün oluşum oranı, MS besin ortamındaki IBA konsantrasyonundan ve eksplant tipinden etkilenmiştir. En yüksek sürgün oluşum oranı Rabi-01 klonuna ait yapraktan elde edilen kalluslarda ve 3 mg/L BA içeren ortamlar üzerinde elde edilmiştir. Adventif sürgünler köklendirilmiş, dış koşullara alıştırılmış ve daha sonra da araziye aktarılarak meyve üretimine kadar gözlenmişlerdir.
Anahtar Kelime: Konular:
Üç değişik çilek (Fragaria sp.) klonunda yaprak sapı, boğum ve stolon parçalarından kallus kültürü
Öz: The present investigation was conducted to study the effects of auxin and cytokinin on callus induction from leaf blade, nodal, and runner segments of 3 strawberry clones. Among the 32 media combinations tested, 4.0 mg/L NAA + 1.5 mg/L IBA yielded the highest percentage of callus in all types of explants. The rate of adventitious shoot regeneration from callus was also influenced by explant type and concentration of BA in MS medium. The highest shoot regeneration rate was observed in leaf derived callus of clone Rabi-01 on media having 3 mg/L BA. Adventitious shoots were rooted, acclimatized, and subsequently transplanted to the field and observed until fruit production.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | BISWAS M, ROY U, ISLAM R, HOSSAIN M (2010). Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. , 75 - 80. |
| Chicago | BISWAS Manosh Kumar,ROY Uthpal Krishna,ISLAM Rafiul,HOSSAIN Monzur Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. (2010): 75 - 80. |
| MLA | BISWAS Manosh Kumar,ROY Uthpal Krishna,ISLAM Rafiul,HOSSAIN Monzur Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. , 2010, ss.75 - 80. |
| AMA | BISWAS M,ROY U,ISLAM R,HOSSAIN M Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. . 2010; 75 - 80. |
| Vancouver | BISWAS M,ROY U,ISLAM R,HOSSAIN M Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. . 2010; 75 - 80. |
| IEEE | BISWAS M,ROY U,ISLAM R,HOSSAIN M "Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones." , ss.75 - 80, 2010. |
| ISNAD | BISWAS, Manosh Kumar vd. "Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones". (2010), 75-80. |
| APA | BISWAS M, ROY U, ISLAM R, HOSSAIN M (2010). Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. Turkish Journal of Biology, 34(1), 75 - 80. |
| Chicago | BISWAS Manosh Kumar,ROY Uthpal Krishna,ISLAM Rafiul,HOSSAIN Monzur Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. Turkish Journal of Biology 34, no.1 (2010): 75 - 80. |
| MLA | BISWAS Manosh Kumar,ROY Uthpal Krishna,ISLAM Rafiul,HOSSAIN Monzur Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. Turkish Journal of Biology, vol.34, no.1, 2010, ss.75 - 80. |
| AMA | BISWAS M,ROY U,ISLAM R,HOSSAIN M Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. Turkish Journal of Biology. 2010; 34(1): 75 - 80. |
| Vancouver | BISWAS M,ROY U,ISLAM R,HOSSAIN M Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones. Turkish Journal of Biology. 2010; 34(1): 75 - 80. |
| IEEE | BISWAS M,ROY U,ISLAM R,HOSSAIN M "Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones." Turkish Journal of Biology, 34, ss.75 - 80, 2010. |
| ISNAD | BISWAS, Manosh Kumar vd. "Callus culture from leaf blade, nodal, and runner segments of three strawberry (Fragaria sp.) clones". Turkish Journal of Biology 34/1 (2010), 75-80. |
