Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus

YALÇIN, H. Tansel; KAYA, GÜLEN İREM; ÖNÜR, Mustafa Ali; YAVAŞOĞLU, N. Ülkü Karabay; SARIKAYA, Buket; SOMER, Nehir Ünver; KONYALIOĞLU, Sibel

Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus

Gülen İrem KAYA, (Ege Üniversitesi, İzmir, Türkiye)

Nehir Ünver SOMER, (Ege Üniversitesi, İzmir, Türkiye)

Sibel KONYALIOĞLU, (Ege Üniversitesi, İzmir, Türkiye)

H. Tansel YALÇIN, (Ege Üniversitesi, İzmir, Türkiye)

N. Ülkü Karabay YAVAŞOĞLU, (Ege Üniversitesi, İzmir, Türkiye)

Buket SARIKAYA, (Ege Üniversitesi, İzmir, Türkiye)

Mustafa Ali ÖNÜR (Ege Üniversitesi, İzmir, Türkiye)

Turkish Journal of Biology

7 0

Yıl: 2010 Cilt: 34 Sayı: 2 Sayfa Aralığı: 139 - 146 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus

Öz:

Ranunculus marginatus d’Urv. var. trachycarpus (Fisch. & Mey.) Azn. ve R. sprunerianus Boiss. bitkilerinin hekzan, etil asetat, metanol ve su ekstrelerinin antioksidan ve antibakteriyal aktiviteleri in vitro olarak test edilmiştir. Ekstrelerin antioksidan aktivitesi DPPH radikal süpürücü ve Troloks eşdeğeri antioksidan kapasite yöntemleriyle tayin edilmiştir. Her iki yöntemde de metanol ekstreleri en yüksek antioksidan aktiviteyi göstermiştir. Ekstrelerdeki total fenol miktarı Folin- Ciocalteau reaktifi kullanılarak kolorimetrik olarak tayin edilmiştir. Ekstrelerin total flavonoid içeriği spektroskopik bir yöntem kullanılarak değerlendirilmiştir. Antioksidan aktivite deneylerinin sonuçları, ekstrelerin total fenol ve flavonoid miktarları ile uyumludur. Antibakteriyel aktivite tayininde disk difüzyon ve mikrodilüsyon teknikleri kullanılmıştır. Disk difüzyon yönteminde bütün ekstreler test edilen bakterilere karşı aktivite göstermiştir. Her iki Ranunculus türünün ekstrelerinin minimal inhibitör konsantrasyonlarının (MİK) 128-256 μg/mL arasında olduğu bulunmuştur.

Anahtar Kelime: antibakteriyel özellikler flavonoidler fenoller Ranunculus Ranunculus sprunerianus mikroorganizmalar antioksidanlar Ranunculus marginatus

Konular: Biyoloji

Ranunculus marginatus var. trachycarpus ve R. sprunerianus’un antioksidan ve antibakteriyal aktiviteleri

Öz:

Hexane, ethyl acetate, methanol, and aqueous extracts of Ranunculus marginatus d’Urv. var. trachycarpus (Fisch. & Mey.) Azn. and R. sprunerianus Boiss. were tested in vitro for their antioxidant and antibacterial activities. Antioxidant activity of the extracts was determined by DPPH radical scavenging and Trolox equivalent antioxidant capacity assays. Methanol extracts showed the highest antioxidant activity in both assays. The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteau reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The results obtained in the antioxidant activity tests were in positive correlation with the total phenolic and flavonoid contents of the extracts. An antibacterial activity analysis was carried out using paper disk diffusion and micro-well dilution techniques. All of the extracts displayed antibacterial activity against the tested bacteria in the disk diffusion method. The minimal inhibitory concentrations (MICs) of all the extracts of both Ranunculus species were found to be between 128 and 256 μg/mL.

Anahtar Kelime: flavonoids phenols Ranunculus Ranunculus sprunerianus microorganisms antioxidants Ranunculus marginatus antibacterial properties

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

TRDizin Dışındaki Atıf Sayıları

OpenCitations: 0

OpenAlex: 0

Crossref: 0

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APA	KAYA G, SOMER N, KONYALIOĞLU S, YALÇIN H, YAVAŞOĞLU N, SARIKAYA B, ÖNÜR M (2010). Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. , 139 - 146.
Chicago	KAYA GÜLEN İREM,SOMER Nehir Ünver,KONYALIOĞLU Sibel,YALÇIN H. Tansel,YAVAŞOĞLU N. Ülkü Karabay,SARIKAYA Buket,ÖNÜR Mustafa Ali Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. (2010): 139 - 146.
MLA	KAYA GÜLEN İREM,SOMER Nehir Ünver,KONYALIOĞLU Sibel,YALÇIN H. Tansel,YAVAŞOĞLU N. Ülkü Karabay,SARIKAYA Buket,ÖNÜR Mustafa Ali Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. , 2010, ss.139 - 146.
AMA	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. . 2010; 139 - 146.
Vancouver	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. . 2010; 139 - 146.
IEEE	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M "Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus." , ss.139 - 146, 2010.
ISNAD	KAYA, GÜLEN İREM vd. "Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus". (2010), 139-146.

APA	KAYA G, SOMER N, KONYALIOĞLU S, YALÇIN H, YAVAŞOĞLU N, SARIKAYA B, ÖNÜR M (2010). Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology, 34(2), 139 - 146.
Chicago	KAYA GÜLEN İREM,SOMER Nehir Ünver,KONYALIOĞLU Sibel,YALÇIN H. Tansel,YAVAŞOĞLU N. Ülkü Karabay,SARIKAYA Buket,ÖNÜR Mustafa Ali Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology 34, no.2 (2010): 139 - 146.
MLA	KAYA GÜLEN İREM,SOMER Nehir Ünver,KONYALIOĞLU Sibel,YALÇIN H. Tansel,YAVAŞOĞLU N. Ülkü Karabay,SARIKAYA Buket,ÖNÜR Mustafa Ali Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology, vol.34, no.2, 2010, ss.139 - 146.
AMA	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology. 2010; 34(2): 139 - 146.
Vancouver	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turkish Journal of Biology. 2010; 34(2): 139 - 146.
IEEE	KAYA G,SOMER N,KONYALIOĞLU S,YALÇIN H,YAVAŞOĞLU N,SARIKAYA B,ÖNÜR M "Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus." Turkish Journal of Biology, 34, ss.139 - 146, 2010.
ISNAD	KAYA, GÜLEN İREM vd. "Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus". Turkish Journal of Biology 34/2 (2010), 139-146.