Yıl: 2010 Cilt: 16 Sayı: 2 Sayfa Aralığı: 103 - 107 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study

Öz:
Abdominal kompartman sendromu sonrası gelişen akut böbrek yetersizliğinin renal venöz basıncın artışı ile ilişkili olduğu düşünülmektedir. Bu çalışmada, değişik karın içi basınç değerlerinde renal tübüler ve glomerüler hücrelerdeki histopatolojik değişiklikler ile plazma ve renal doku sitokin düzeylerindeki değişiklikler araştırıldı ve karşılaştırıldı. GEREÇ VE YÖNTEM On sekiz Spraque-Dawley cinsi sıçan rastgele üç gruba ayrıldı: Sham grubu, basıncın 20 ve 30 mmHg düzeyinde 60 dakika tutulduğu grup 2 ve grup 3. Deneklerin, sol böbrekleri ve intrakardiyak kan örnekleri işlem sonunda alındı. Tümör nekroz faktör alfa (TNF-α) ve interlökin 6 (IL-6) düzeyleri hem renal dokuda hem de plazmada araştırıldı. Renal dokular, histopatolojik değişiklikler açısından da değerlendirildi. BULGULAR Sham grubu çalışma grupları ile karşılaştırıldığında, renal doku ve plazma TNF-α, IL-6 düzeylerinin anlamlı olarak arttığı saptandı (p<0,05). Renal doku değişiklikleri değerlendirildiğinde, total histopatolojik skorların arttığı (p<0,05) tübüler değişikliklerin, glomerüler değişikliklerden daha belirgin olduğu saptandı. SONUÇ Sonuç olarak, karın içi basınç artışı, doğrusal olarak renal tübüler histopatolojik değişiklikleri arttırmaktadır. Sitokinler abdominal kompartman sendromunda gelişen akut böbrek yetersizliğinde rol oynuyor olabilirler.
Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Üroloji ve Nefroloji Pediatri

Karın içi basıncın hızla artışı sonrası gelişen renal histopatolojik ve sitokin değişiklikleri: Bir hayvan çalışması

Öz:
BACKGROUND The reason for acute renal failure (ARF) in abdominal compartment syndrome (ACS) is thought to be due to the increase in renal venous pressure. In this study, the changes in plasma and renal tissue cytokine levels and histopathologic changes in renal tubular and glomerular cells were evaluated and compared in a model of acute elevation in abdominal tension with different pressures. METHODS Eighteen Sprague-Dawley rats were randomly assigned into three groups: Sham-operated rats and rats in Groups 1 and 2, in which 20 and 30 mmHg of intra-abdominal pressure (IAP) was applied for 60 minutes, respectively. Left kidneys of the animals and intracardiac blood samples were taken at the end of the procedures. Tumor necrosis factor-alpha (TNF-&#945;) and interleukin-6 (IL-6) levels were investigated in plasma and renal tissue samples. Renal tissues were also evaluated for histopathologic changes. RESULTS Renal tissue and plasma TNF-&#945; and IL-6 levels were found to be significantly increased when the sham-operated group was compared to the study groups (p<0.05). Renal tissue changes showed that the total histopathologic scores were significantly increased in study groups compared to the sham-operated group (p<0.05); tubular changes were more prominent than glomerular changes. CONCLUSION Abdominal tension linearly can cause renal tubular histopathologic changes. Cytokines may play a role in ARF due to ACS.
Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Üroloji ve Nefroloji Pediatri
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Saggi BH, Sugerman HJ, Ivatury RR, Bloomfeld GL. Abdominal compartment syndrome. J Trauma 1998;45:597-609.
  • 2. Sivarajah A, Chatterjee PK, Hattori Y, Brown PA, Stewart KN, Todorovic Z, et al. Agonists of peroxisome-proliferator activated receptor-alpha (clofbrate and WY14643) reduce renal ischemia/reperfusion injury in the rat. Med Sci Monit 2002;8:BR532-9.
  • 3. Bradley SE, Bradley GP. The effect of increased intraabdominal pressure on renal function in man. J Clin Invest 1947;26:1010-22.
  • 4. Shenasky JH 2nd. The renal hemodynamic and functional effects of external counterpressure. Surg Gynecol Obstet 1972;134:253-8.
  • 5. Platell CF, Hall J, Clarke G, Lawrence-Brown M. Intraabdominal pressure and renal function after surgery to the abdominal aorta. Aust N Z J Surg 1990;60:213-6.
  • 6. Celoria G, Steingrub J, Dawson JA, Teres D. Oliguria from high intra-abdominal pressure secondary to ovarian mass. Crit Care Med 1987;15:78-9.
  • 7. Kirsch AJ, Hensle TW, Chang DT, Kayton ML, Olsson CA, Sawczuk IS. Renal effects of CO2 insuffation: oliguria and acute renal dysfunction in a rat pneumoperitoneum model. Urology 1994;43:453-9.
  • 8. Harman PK, Kron IL, McLachlan HD, Freedlender AE, Nolan SP. Elevated intra-abdominal pressure and renal function. Ann Surg 1982;196:594-7.
  • 9. Doty JM, Saggi BH, Blocher CR, Fakhry I, Gehr T, Sica D, et al. Effects of increased renal parenchymal pressure on renal function. J Trauma 2000;48:874-7.
  • 10. Doty JM, Saggi BH, Sugerman HJ, Blocher CR, Pin R, Fakhry I, et al. Effect of increased renal venous pressure on renal function. J Trauma 1999;47:1000-3.
  • 11. Wakefeld CH, Barclay GR, Fearon KC, Goldie AS, Ross JA, Grant IS, et al. Proinfammatory mediator activity, endogenous antagonists and the systemic infammatory response in intra-abdominal sepsis. Scottish Sepsis Intervention Group. Br J Surg 1998;85:818-25.
  • 12. Hammond JM, Potgieter PD. The infuence of surgery on cytokines in patients with intra-abdominal sepsis. Anaesth Intensive Care 1996;24:430-4.
  • 13. Tang GJ, Kuo CD, Yen TC, Kuo HS, Chan KH, Yien HW, et al. Perioperative plasma concentrations of tumor necrosis factor-alpha and interleukin-6 in infected patients. Crit Care Med 1996;24:423-8.
APA Akbulut G, ALTINDİŞ M, AKPTEPE F, Serteser M, Dilek O (2010). Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. , 103 - 107.
Chicago Akbulut Gökhan,ALTINDİŞ Mustafa,AKPTEPE Fatma,Serteser Mustafa,Dilek Osman Nuri Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. (2010): 103 - 107.
MLA Akbulut Gökhan,ALTINDİŞ Mustafa,AKPTEPE Fatma,Serteser Mustafa,Dilek Osman Nuri Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. , 2010, ss.103 - 107.
AMA Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. . 2010; 103 - 107.
Vancouver Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. . 2010; 103 - 107.
IEEE Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O "Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study." , ss.103 - 107, 2010.
ISNAD Akbulut, Gökhan vd. "Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study". (2010), 103-107.
APA Akbulut G, ALTINDİŞ M, AKPTEPE F, Serteser M, Dilek O (2010). Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. Ulusal Travma ve Acil Cerrahi Dergisi, 16(2), 103 - 107.
Chicago Akbulut Gökhan,ALTINDİŞ Mustafa,AKPTEPE Fatma,Serteser Mustafa,Dilek Osman Nuri Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. Ulusal Travma ve Acil Cerrahi Dergisi 16, no.2 (2010): 103 - 107.
MLA Akbulut Gökhan,ALTINDİŞ Mustafa,AKPTEPE Fatma,Serteser Mustafa,Dilek Osman Nuri Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. Ulusal Travma ve Acil Cerrahi Dergisi, vol.16, no.2, 2010, ss.103 - 107.
AMA Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. Ulusal Travma ve Acil Cerrahi Dergisi. 2010; 16(2): 103 - 107.
Vancouver Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study. Ulusal Travma ve Acil Cerrahi Dergisi. 2010; 16(2): 103 - 107.
IEEE Akbulut G,ALTINDİŞ M,AKPTEPE F,Serteser M,Dilek O "Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study." Ulusal Travma ve Acil Cerrahi Dergisi, 16, ss.103 - 107, 2010.
ISNAD Akbulut, Gökhan vd. "Renal cytokine and histopathologic changes following acutely increased intra-abdominal pressure: An animal study". Ulusal Travma ve Acil Cerrahi Dergisi 16/2 (2010), 103-107.