Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity

TIPIRDAMAZ, Rukiye; BAYSAL FURTANA, GÖKÇEN

Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity

Gökçen Baysal FURTANA, (Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Ankara Türkiye)

Rukiye TIPIRDAMAZ (Hacettepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2010 Cilt: 34 Sayı: 3 Sayfa Aralığı: 287 - 296 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity

Öz:

Bu çalışmada salatalık (Cucumis sativus L.) çeşitlerinde (Çengelköy, Anadolu F1, Beith Alpha) tuzluluğun, büyüme, oransal su kapsamı, klorofil miktarı, inorganik iyonlar, prolin birikimi, lipid peroksidasyonu ile süperoksit dismutaz (SOD), askorbat peroksidaz (APOX) ve katalaz (CAT) antioksidant enzimlerine etkisi araştırılmıştır. Salatalık çeşitleri kontrollü koşullarda, perlit ortamında, ½ Hoagland besin çözeltisi ile sulanarak 35 gün büyütülmüştür. Bu periyot sonunda; fidelere 150 mM NaCl uygulanmıştır ve tuz uygulamasının 7. ve 14. gününde bitkiler hasat edilmiştir. Taze ve kuru ağırlıkları, oransal su kapsamları (OSK), inorganik iyonlar, toplam klorofil, prolin ve malondialdehid (MDA) miktarları ile süperoksit dismutaz (SOD), katalaz (CAT), askorbat peroksidaz (AP) enzim aktiviteleri belirlenmiştir. Genel olarak, uygulanan tuzluluk tuz muamele süreci ve çeşitli farklılıklara bağlı olarak etkilenmiştir. Ayrıca tuzlulukla birlikte prolin içeği de artmış, fakat Çengelköy çeşidinde prolin birikimi diğer çeşitlere oranla daha az olmuştur. Sonuçlar, Çengelköy çeşidinin artan antioksidant enzim aktivitesine bağlı olarak oksidatif hasara karşı koruma mekanizmasının Beith Alpha ve Anadolu F1 çeşitlerinden daha iyi olabileceğini göstermiştir. Muhtemelen, Çengelköy’ün içerdiği daha düşük MDA ve prolin içeriği ile $Na^+ and Cl^-$ konsantrasyonu ve daha yüksek K+ konsantrasyonu ile oransal su kapsamı, toplam klorofil içeriği, süperoksit dismutaz, katalaz ve askorbat peroksidaz aktivitesi bu çeşidin tuza Beith Alpha ve Anadolu F1’e oranla daha dayanıklı olmasını sağlamaktadır.

Anahtar Kelime: malondialdehit süperoksit dismutaz stres yanıtı askorbat peroksidaz hıyar prolin tuzluluk enzim aktivitesi klorofil Cucumis sativus antioksidanlar katalaz

Konular: Biyoloji

Üç salatalık (Cucumis sativus L.) çeşidinin tuzluluğa karşı fizyolojik ve antioksidant tepkileri

Öz:

The effects of salinity on the growth, relative water content (RWC), chlorophyll content, inorganic ions, proline accumulation, lipid peroxidation and antioxidant enzymes, superoxide dismutase (SOD), ascorbate peroxidase (APOX), and catalase (CAT) of 3 cucumber (Cucumis sativus L.) cultivars (Çengelköy, Anadolu F1, and Beith Alpha) were investigated. Cucumber cultivars were grown in perlite culture and irrigated with half-strength Hoagland nutrient solution for a period of 35 days under controlled conditions. After this period, seedlings were treated with 150 mM NaCl solution and the plants were harvested on day 7 and day 14 of salt treatment. The fresh and dry weights, RWC, inorganic ions, total chlorophyll, proline and malondialdehyde (MDA) content, and SOD, CAT, and APOX activities were determined. In general, the applied salinity affected all of the considered parameters depending on the duration of salt treatment and the cultivars analyzed. Proline content also increased with salinity, but accumulation of proline was lower in Çengelköy than in the other cultivars. The results suggest that the protection mechanism against oxidative damage due to induced activity of antioxidant enzymes may be better in Çengelköy than in Beith Alpha or Anadolu F1. The higher $K^+$ concentrations, RWC, total chlorophyll content, and SOD, CAT, and APOX activities, together with the lower MDA, proline content, and $Na^+ and Cl^-$ concentrations detected in Çengelköy might make this cultivar more salt-tolerant than Beith Alpha or Anadolu F1.

Anahtar Kelime: enzyme activity chlorophyll Cucumis sativus antioxidants catalase malondialdehyde superoxide dismutase stress response ascorbate peroxidase cucumbers proline salinity

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAYSAL FURTANA G, TIPIRDAMAZ R (2010). Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. , 287 - 296.
Chicago	BAYSAL FURTANA GÖKÇEN,TIPIRDAMAZ Rukiye Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. (2010): 287 - 296.
MLA	BAYSAL FURTANA GÖKÇEN,TIPIRDAMAZ Rukiye Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. , 2010, ss.287 - 296.
AMA	BAYSAL FURTANA G,TIPIRDAMAZ R Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. . 2010; 287 - 296.
Vancouver	BAYSAL FURTANA G,TIPIRDAMAZ R Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. . 2010; 287 - 296.
IEEE	BAYSAL FURTANA G,TIPIRDAMAZ R "Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity." , ss.287 - 296, 2010.
ISNAD	BAYSAL FURTANA, GÖKÇEN - TIPIRDAMAZ, Rukiye. "Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity". (2010), 287-296.

APA	BAYSAL FURTANA G, TIPIRDAMAZ R (2010). Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. Turkish Journal of Biology, 34(3), 287 - 296.
Chicago	BAYSAL FURTANA GÖKÇEN,TIPIRDAMAZ Rukiye Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. Turkish Journal of Biology 34, no.3 (2010): 287 - 296.
MLA	BAYSAL FURTANA GÖKÇEN,TIPIRDAMAZ Rukiye Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. Turkish Journal of Biology, vol.34, no.3, 2010, ss.287 - 296.
AMA	BAYSAL FURTANA G,TIPIRDAMAZ R Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. Turkish Journal of Biology. 2010; 34(3): 287 - 296.
Vancouver	BAYSAL FURTANA G,TIPIRDAMAZ R Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity. Turkish Journal of Biology. 2010; 34(3): 287 - 296.
IEEE	BAYSAL FURTANA G,TIPIRDAMAZ R "Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity." Turkish Journal of Biology, 34, ss.287 - 296, 2010.
ISNAD	BAYSAL FURTANA, GÖKÇEN - TIPIRDAMAZ, Rukiye. "Physiological and antioxidant response of three cultivars of cucumber (Cucumis sativus L.) to salinity". Turkish Journal of Biology 34/3 (2010), 287-296.