Aim: This study aimed to determine the level of occupational fatigue in healthcare professionals during the COVID-19 pandemic process and assess some variables considered to have a relationship with it. Material and Method: This study used a descriptive cross-sectional design and was carried out on physicians and nurses between June and July 2020. The study data were collected using a questionnaire with items questioning some socio-demographic data of the healthcare professionals, characteristics of their workplace and working conditions, and contact with patients with COVID-19, and the items of the Occupational Fatigue-Exhaustion Recovery Scale. The study group consisted of 308 females and 98males. In non-normal distributions, the Mann-Whitney U test was used for variables with two groups and Kruskal–Wallis-H test for variables with more than two groups.Results: The mean scores of the healthcare professionals from the chronic fatigue and acute fatigue sub-domains of the Occupational Fatigue Exhaustion Recovery Scale were 60.65±29.10, 65.52±21.64 and 45.09±19.1, respectively. In the study group, fatigue levels were higher in women who were aged 49 years or younger, had weekly working hours of more than 40 hours, and came into contact with COVID 19 patients every day.. Conclusion: The fatigue and burnout levels of healthcare professionals who are directly involved in the care of patients with COVID-19 during the pandemic process were above moderate levels, and the level of their recovery was at a moderate level. It is recommended that necessary administrative initiatives should be taken to improve healthcare workers’ rights, whose working conditions are getting more difficult in the pandemic environment, their needs for rest should be planned, and that working environments that will ensure that not only the risk of contamination but also the risk factors that will arise due to fatigue are under control should be created.