Cytotoxic and Apoptotic Effects of the Combination  of Borax (Sodium Tetraborate) and 5-Fluorouracil on  DLD-1 Human Colorectal Adenocarcinoma Cell Line

Kaya-Sezginer, Ecem; KIRLANGIÇ, Ömer Faruk; Yavuz, Ozlem; Gur, Serap; Ören, Sema

doi:10.4274/tjps.galenos.2021.29726

Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line

Ömer Faruk KIRLANGIÇ, (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye)

Ecem KAYA-SEZGİNER, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Sema ÖREN, (Türkiye Sağlık Bilimleri Üniversitesi, Gülhane Sağlık Bilimleri Enstitüsü Araştırma Merkezi, Ankara, Türkiye)

Serap Gur, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Özlem YAVUZ (Türkiye Sağlık Bilimleri Üniversitesi, Gülhane Eğitim ve Araştırma Hastanesi, Klinik Biyokimya Anabilim Dalı, Ankara, Türkiye)

Turkish Journal of Pharmaceutical Sciences

3 0

Yıl: 2022 Cilt: 19 Sayı: 4 Sayfa Aralığı: 371 - 376 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2021.29726 İndeks Tarihi: 28-09-2022

Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line

Öz:

Objectives: Colorectal cancer (CRC) remains a crucial health problem due to the toxicity of 5-Fluorouracil (5-FU) as first-line chemotherapy agent for treating CRC. The anticancer effects of boron and its compounds have been shown in various cell lines. This study aimed to examine the cytotoxic and apoptotic effects of borax (sodium tetraborate) alone or along with 5-FU on human CRC cells, DLD-1. Materials and Methods: Cytotoxicity and apoptosis were determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, 4’,6-diamidino-2-phenylindole and annexin V/propidium iodide staining. Results: The results showed that combined treatment revealed a significant time- and concentration-dependent cytotoxic effect on DLD-1 cells compared with borax or 5-FU treatment alone. The combination of borax and 5-FU induced a clear increase in the early apoptotic cell percentage, compared to the cells treated with monotherapies. Additionally, a significant increase in condensed and fragmented nuclei was detected in DLD-1 cells treated with the combination treatment compared with borax or 5-FU alone. Conclusion: Our current findings suggest that the combination of borax with 5-FU has a strong cytotoxic and apoptotic effect on the human CRC DLD-1 cells

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KIRLANGIÇ Ö, Kaya-Sezginer E, Ören S, Gur S, Yavuz O (2022). Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. , 371 - 376. 10.4274/tjps.galenos.2021.29726
Chicago	KIRLANGIÇ Ömer Faruk,Kaya-Sezginer Ecem,Ören Sema,Gur Serap,Yavuz Ozlem Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. (2022): 371 - 376. 10.4274/tjps.galenos.2021.29726
MLA	KIRLANGIÇ Ömer Faruk,Kaya-Sezginer Ecem,Ören Sema,Gur Serap,Yavuz Ozlem Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. , 2022, ss.371 - 376. 10.4274/tjps.galenos.2021.29726
AMA	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. . 2022; 371 - 376. 10.4274/tjps.galenos.2021.29726
Vancouver	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. . 2022; 371 - 376. 10.4274/tjps.galenos.2021.29726
IEEE	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O "Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line." , ss.371 - 376, 2022. 10.4274/tjps.galenos.2021.29726
ISNAD	KIRLANGIÇ, Ömer Faruk vd. "Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line". (2022), 371-376. https://doi.org/10.4274/tjps.galenos.2021.29726

APA	KIRLANGIÇ Ö, Kaya-Sezginer E, Ören S, Gur S, Yavuz O (2022). Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. Turkish Journal of Pharmaceutical Sciences, 19(4), 371 - 376. 10.4274/tjps.galenos.2021.29726
Chicago	KIRLANGIÇ Ömer Faruk,Kaya-Sezginer Ecem,Ören Sema,Gur Serap,Yavuz Ozlem Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. Turkish Journal of Pharmaceutical Sciences 19, no.4 (2022): 371 - 376. 10.4274/tjps.galenos.2021.29726
MLA	KIRLANGIÇ Ömer Faruk,Kaya-Sezginer Ecem,Ören Sema,Gur Serap,Yavuz Ozlem Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. Turkish Journal of Pharmaceutical Sciences, vol.19, no.4, 2022, ss.371 - 376. 10.4274/tjps.galenos.2021.29726
AMA	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. Turkish Journal of Pharmaceutical Sciences. 2022; 19(4): 371 - 376. 10.4274/tjps.galenos.2021.29726
Vancouver	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line. Turkish Journal of Pharmaceutical Sciences. 2022; 19(4): 371 - 376. 10.4274/tjps.galenos.2021.29726
IEEE	KIRLANGIÇ Ö,Kaya-Sezginer E,Ören S,Gur S,Yavuz O "Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line." Turkish Journal of Pharmaceutical Sciences, 19, ss.371 - 376, 2022. 10.4274/tjps.galenos.2021.29726
ISNAD	KIRLANGIÇ, Ömer Faruk vd. "Cytotoxic and Apoptotic Effects of the Combination of Borax (Sodium Tetraborate) and 5-Fluorouracil on DLD-1 Human Colorectal Adenocarcinoma Cell Line". Turkish Journal of Pharmaceutical Sciences 19/4 (2022), 371-376. https://doi.org/10.4274/tjps.galenos.2021.29726